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Objective: To study the effects of Total Alkaloid of Menispermum on apoptosis, cell cycle and invasive ablity to the Hela cell. To investigate the antitumor mechanisms of TAM.

目的：研究北豆根总碱对宫颈癌细胞Hela凋亡、细胞周期及侵袭性的影响，探讨北豆根总碱抗肿瘤作用机制，为抗肿瘤新药的研究和开发提供科学依据。

Furthermore, out of 497 fAFLP markers, 80 special bands were found to be able to distinguish the four groups from each other and may be applied for germplasm characterization and molecular assistant classification of Meretrix clam.4 Molecular classification of two species of Meretrix clam based on fAFLP and ITS sequences4.1 The results of fAFLP maker analysis of S, G and W showed that each group had their own specific loci among which there were 53 special loci in W group, much more than those of S group (14) and G group (21). Among the 53 loci, nine were all dominant loci. These unique loci could be taken as molecular markers to distinguish W from other groups. The genetic similarity indexes and distance matrix between S and G groups were 0.9585 and 0.0424 respectively, but the genetic similarity indexes and distance matrix between W group and S or G group was 0.7939 or 0.7941, and 0.2308 or 0.2305 respectively. The results revealed that significant difference existed between W and S or G groups in molecular genetic structure. The phylogenetic trees by the methods of UPGMA and NJ also indicated that S and G populations were very closely related, while W population was a relatively independent cluster, lying beyond the species which S and G belong to.4.2 The internal transcribed spacer region of the rDNA from S group, G group and W group were PCR amplified and sequenced. The results showed that the size of ITS ranged between 1266-1269bp in W group, while those in G and S groups were 1614bp and 1520bp respectively. The GC content ranged 62.32-62.62% in W group while it was 61.77% in G group. The genetic distances between three populations of W group were 0.001～0.003, but it was 0.110 or 0.147 respectively between W group and G group or S group. Phylogenetic trees by NJ method also showed that G group was very closely related to S group, while W group was a relatively independent cluster.

在457个总扩增位点中找出了53个W的特有位点，远多于S群体(14)和G(21)群体，而且在53个特有位点中有9个出现频率为100%的位点，这些位点可以作为区分其它2个群体的特征性标记；S– G群体特有的位点有112个，其中有4个位点出现频率为100%，可作为S– G群体区别于W群体的特征性标记。S群体和G群体间的遗传相似性系数为0.9585，遗传距离只有0.0424，在NJ和UPGMA法构建的亲缘关系的树状图上均首先聚在一起，说明二者的亲缘关系很近，应属于种内群体间的关系；而W与S和G的遗传相似性系数均较小(0.7939和0.7941)，相对遗传距离很大而且十分相近(0.2308和0.2305)，在亲缘关系树状图上单独分出一支，也表明W与S和G群体间的亲缘关系较远。4.2 ITS序列比较分析通过对白壳文蛤、山东文蛤和广西文蛤的ITS序列扩增电泳、PCR-RFLP分析和ITS序列分析发现，W的ITS序列长度在1266-1269 bp，而S和与G的ITS序列总长度分别为1520 bp和1614 bp；从ITS1和ITS2长度来看，W分别为739-741 bp和316-317 bp，S为895 bp和414 bp，G为987 bp和416 bp；而从ITS碱基组成来看，W的GC含量在62.32-62.62%之间，而G群体为61.77%。W的3个壳色不同群体间的遗传距离仅0.001、0.002和0.003，S与G群体间的遗传距离是0.010，说明W群体内变异很小，而S与G群体间已出现明显的遗传分化，但还均属于种内群体间的遗传变异；而W与G和S的遗传距离分别达到0.110、0.147，两个类群差异显著，已远超出种内群体间的遗传变异。

The result showed that except for transverse diameter, metaxenia effects on fruit longitudinal diameter, fruit shape index, fruit weight, flesh recovery rate, total soluble solids, contents of sugars, acid and Vitamin c in juice, and contents of anthocyanins and chlorophyll in peel were significant based on Duncan's multiple range tests.

结果表明，除果实横径外，在纵径、果形指数、单果质量、可食率、可溶性固形物、总糖、酸、维生素C、果皮花青苷、叶绿素含量等方面花粉直感表现明显，经邓肯氏多重比较测验，差异达显著水平；桂味荔枝自交授粉果在果形指数、总糖含量方面与其它品种授粉果差异不显著。

Microbiotic crusts had a high capacity of evaporation than the shifting sand dunes. During the evaporation process of one week after rainfall, the total evaporation in artificial vegetation regions constructed in 1956, 1964, and 1981 were 6.05, 4.99 and 4.23 times respectively as the total evaporation in shifting sand dunes.

微生物结皮有着比流沙区更高的蒸发能力，在雨后一周的蒸发过程中，1956、1964和1981年人工植被区的总蒸发量分别是流沙区总蒸发量的6.05、4.99和4.23倍。

Nonetheless, seeing our own life, we tend to minish our happiness and magnify our vexation, thereby feeling more and more unsatisfied with our present life.

而在看待自己生活时，人们总喜欢缩小自己的幸福，扩大自己的烦恼，所以我们对自己的生活总有太多的不满。

Nonetheless, seeing our own life, we tend to minish our happiness and magnifyour vexation, thereby feeling more and more unsatisfied with our present life.

而在看待自己生活时，人们总喜欢缩小自己的幸福，扩大自己的烦恼，所以我们对自己的生活总有太多的不满。

The effects of the total saponin extract from the shoot s of Momordica charantia L.

从苦瓜茎叶提取总皂甙并经体外细胞试验表明：总皂甙在浓度为0。

The results indicated that the strain was Mracemosus fresenius which belonged to Racemousus, Muco, Mucoraceae, Mucorales, Phycomycetes in Fungi. Its growth temperature was under 37℃, and the optimal temperature was determined to 28℃.

结果表明，该菌株为真菌门、藻菌纲、毛霉目、毛霉科、毛霉属、总状枝毛霉组中的总状枝毛霉。

The most dominant species, Coilia nasus, was accounted for 55.19%, and then Pseudolaubuca sinensis (16.99%), Liza haematocheila (10.94%), Tridentiger trigonocephalus (3.92%), Acanthogobius ommaturus (2.67%), Siniperca chuatsi (1.68%), Sillago japonica (1.68%), Acentrogobius pflaumii (0.99%), Periophthalmus modestus (0.99%), Acanthogobius flavimanus (0.89%). These 10 species were accounted for 96% in the total abundance.

其中鳀科的刀鲚占总个体数的55.19%，其后依次为银飘鱼(16.99%)、鱼(10.94%)、纹缟虾虎鱼(3.92%)、斑尾刺虾虎角(2.67%)、鳜(1.68%)、少鳞(l.68%)、普氏细棘虾虎鱼(0.99%)、弹涂鱼(0.99%)、黄鳍刺虾虎鱼(0.89%)，这10种的个体数占据了总捕获个体数的96%。

Nitrogen species in FCC diesel oil include basic species (such as aniline, quinoline, and their derivatives) and nonbasic species (such as indole, carbazole, and their derivatives), and only 0.1% of the nitrogen compounds are quinoline and about 64% of them are carbazole and their derivatives.

氮化物主要为碱性氮化物和非碱性氮化物两大类型，其中碱性氮化物主要是苯胺及其衍生物，喹啉含量很低，约占总氮质量分数的0.1%，非碱性氮化物主要包括吲哚及其衍生物和咔唑及其衍生物，而咔唑类氮化物一般约占总氮质量分数的64%。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。