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Discussed the subject on the rich symbiont germplasm resource in China and on further research being necessary to exploitation and utilization of the benificial germplasm.

文摘:针对我国主要豆科作物大豆和花生的研究成果,并结合本身研究工作内容,(1)总结了与宿主共生的根瘤菌生物学多样性,包括大、小样本的土著根瘤菌群体数量、分离频率、类型与分布及其菌株―宿主共生混交性与亲和性;(2)评述了根瘤菌―宿主植物共生体双边固氮改良,包括优良菌株的选育、宿主品种资源共生特性的评价与利用及特异性状的选择;(3)讨论了我国这一类共生体资源的丰富性以及有必要进一步加大力度研究有益资源的开发和利用的问题。

For some kinds of vegetables and pasturages, the contents of some heavy metals surpass the relevant quality standards, so when some crops were planted on the substrates, the uneatable species should be selected preferably and if some kinds of vegetables and pasturages must be adopted, the output could not be provided to mankind and also not be used in farming, in order to avoid harming peoples'health.5 The expediency evaluation model of pioneer plant on substrates was established and some plants were studied. The results show that the expediency evaluation of pioneer plants were respectively that clover was expedient to substrate 1, juniper, cotton and fescue were expedient to substrate 2 and juniper, annual bluegrass, fescue, cotton and clover were expedient to substrate 3.6 Some indexes to evaluate the capability of the phytoremediation on heavy metals and the comprehension about hyperaccumulator were put forward.

有多种植物的重金属含量超过了相应的国家标准,因此在基质上种植植物时,尽量选择非食用性植物品种,即使种植了蔬菜和牧草等植物,也不应将收获后的产品供人类食用或用于畜牧生产,以免危害人体健康。5建立和研究了复垦基质先锋植物的适宜性评价模型,结果表明,不同基质中适宜的先锋植物分别如下:基质1为苜蓿,基质2为桧柏、棉花和高羊茅,基质3为桧柏、早熟禾、高羊茅、棉花和苜蓿。6探讨了评价重金属元素植物修复能力的指标体系和"超富集植物"的概念。

The E-TOF will also be feasible and practical to assist chemists in screening the optimal reaction condition or evaluating the overall efficiency of different catalytic systems for a specific asymmetric reaction.4.For the first time,we explored chiralγ-hydroxy-α,β-acetylenic esters,which were prepared via asymmetric alkynylation,as key intermediates to the synthesis of chiralγ-butyrolactones. This convergent and enantioselective approach to chiralγ-butyrolactones is practical and easily handled with protecting group free.

这一概念包括反应产率、对映选择性、配体用量以及反应时间四个重要反应参数,不仅适用同一反应不同催化体系的横向综合性评价,而且适用于某一特定反应催化体系的优化过程,有助于研究人员选择最佳的反应条件。4、我们发展了以手性γ-羟基-α,β-炔酸酯为关键中间体完成了手性γ-丁内酯类天然产物的对映立体选择性全合成,该关键中间体能够以我们的催化体系方便的对映选择性合成,之后的官能团转换方法成熟,易于操作,无需进行繁琐的官能团保护和脱保护过程,绿色经济。

At first, this paper makes a systematic exposition of the content of supply-chain, supply-chain-management, purchasing in supply chain and purchasing management in supply chain environment and builds the theoretical foundation of this paper. Then, Make out the material classification from the acquirability of supply market and essentiality of material. Then based on this conclusion bring forward the proper purchasing strategies for Wuxi Weifu Group Co., Ltd. such as win-win strategy, ensure supply strategy, minimize cost strategy and minimize managerial cost strategy. Then sets up evaluated indexes system of short-term-efficiency-suppliers and long-term-efficiency-suppliers, applying the Fuzzy-evaluation method to evaluate suppliers. At the end of this paper, taking Wuxi Weifu Group Co., Ltd. as a case, comparing the status of its purchasing and supplier management, it puts forward some improvement suggestions to the existing problems in light of the above analysis such as the establishment and improvement of management information system, reengineering of purchasing organization and process, establishment of evaluated indexes system and implement of Just-in-time purchase.

本文首先系统的阐述供应链及供应链管理,供应链管理下的采购和采购管理的内容,从而构筑了本文的理论基础;继而,从物料采购的市场可得性和物料的重要性两种维度将物料分为战略性物料、瓶颈物料、重要物料、一般物料,在此基础上结合无锡威孚公司产品提出了相应的双赢、保障供应、最低成本、管理成本最小等采购策略;接着,建立了短/长期效率型供应商综合评价的指标体系;运用模糊综合评判方法对供应商的选择与评价体系进行了研究;最后,以无锡威孚集团有限公司为案例进行研究,结合前述的理论框架,通过对其采购和供应商管理现状的比较分析,找出不足之处,提出了改进建议和设想,如管理信息系统的建立和完善、采购组织和流程的重构、供应商选择评价体系的建立、即时制采购的实施等方案。

Based on these previous work, in this research, in order to explore the the molecular mechanism of enhanced tumor cell immunogenicity treated by elemene and mytomycin C compositely, we used murine Hca-F ascitic hepatic carcinoma ( high lymphatic metastasis substrain of H22) as an experimental model to compare the immunoprotective effects on Hca-F cells as well as the mechanism of the effects caused by HSP70-peptide complex purified from untreated tumor cells, EC-TCV and BCG; We studied the mechanism of increased tumor cell membrane HSP expression treated with elemene by the techniques of RT-PCR and immunoelectron microscopy; In order to deeply elucidate the antitumor effects and the molecular mechanism of elemene in increasing tumor cell immunogenicity we analyzed and compared the effects of elemene and heat shock on the gene expressions of tumor cell using gene expression profile chip.

本论文在上述研究成果的基础上,选择小鼠肝癌H22的高淋巴道转移亚系Hca-F为模型,进一步比较了未处理肿瘤细胞来源、榄香烯复合瘤苗来源的和卡介苗来源的HSP70-肽复合物对Hca-F的免疫保护效应、效应机制,探讨榄香烯-丝裂霉素复合处理增强肿瘤细胞免疫原性的分子机制;以Hca-F和人肝癌细胞株HepG〓等为模型研究了榄香烯处理增强肿瘤细胞膜热休克蛋白表达的机制;以HepG〓为模型用表达谱基因芯片研究比较了榄香烯与热休克处理对肿瘤细胞基因表达的影响,从而进一步阐明榄香烯抗肿瘤作用及增强肿瘤细胞免疫原性的分子机制。

In contrast to conventional methods, a novel cell sheet-based technique has been proposed .This technique intend to achieve high cell density and function by layering confluent viable cell sheets without the use of acellular scaffolds that limit mass transport. Using thermo-sensitive cell culture surfaces, confluently cultured cells spontaneously detach as intact sheets simply by reducing the culture temperature.

与传统方法相比,有学者提出了一种新的以细胞层为基础的组织工程技术,这种技术作为一种新的选择,利用温敏性培养皿,经简单的温度变化,可以非损伤性的一起获得完整的培养细胞层及其所沉积的细胞外基质,从而避免了使用蛋白水解酶。

Energy may be sublimated through creative art, music, or drama; work may be found dealing with the healing professions; devotion to human welfare in some form could be a splendid corrective measure in redirecting the desire nature; knowledge and understanding of sex, physiologically, psychologically, and esoterically, would certainly serve to clarify the mental pictures so that the person could, to greater degree, understand his problem; establishment of cleanliness as a factor in his physical habits could bring his consciousness into attunement with the ideal of inner and outer purity; and, above all, establishing in the consciousness the goal of love by which all desire impulses find their regeneration.

最好能通过艺术创作,音乐,戏剧来使能量得以升华,选择有关医疗方面的工作,致力于公共福利事业会将欲望引导至正确的方向上。加深对性的认识和理解,在生理上心理上都会潜在地有助于认识自己所存在的问题。养成一种洁净的性方式,作为一种生理习惯因素,它会有助于意识与内外在纯洁的理想形成和谐的统一,更重要的是,将爱的理想植根于意识之中,借此使冲动和欲望得以净化重生。

The data byχ~2 were consistent with the separation ratio as 1: 2: 1 χ~2=4.028<χ_(0.05,2~2=5.99, which further conformed that glandless character was controlled by a incomplete dominance gene, Gl_2~e. 2. SSR markers every about 5cM were selected in the chromosome 12 of our lab genetic linkage map to screen the parent plants. The markers with the polymorphism were used to amplify the DNA of 210 F_2 segregating plants selected randomly and recorded the marker genotypes. After eliminating the segregation distortion markers byχ~2 test, other SSR markers were detected by linkage analysis.

二、参照本实验室海陆种间遗传连锁图谱第12染色体上的SSR标记,平均每5cM左右选择一个标记进行亲本多态性的筛选,筛选出具有多态性的标记位点进一步扩增随机抽取的210个F_2群体单株的DNA,鉴定每个个体的标记基因型。X~2检测剔除偏分离标记后进行连锁分析,发现Gl_2~e基因位于CIR362与NAU5079之间,进一步参考图谱上位于CIR362与NAU5079之间的标记加密图谱,最终将其定位在CIR362和NAU2251b、NAU3860b、STV033之间。

In nonsmokers without pulmonary disease at baseline, there were no severe decreases in lung function attributed to Exubera, and high-resolution chest computed tomography revealed no evidence of notable lung changes. Completed phase 2 and 3 studies that followed patients for up to 4 years indicate that the differences over time in pulmonary function changes between patients treated with Exubera and control patients are small, nonprogressive, clinically insignificant, and reverse after discontinuation of Exubera therapy.

Feb。 9, 2006 --尽管一开始对於肺功能影响的顾虑,美国食品药物管理局仍然批准辉瑞药厂生产的吸入性胰岛素Exubera使用於成人第1、2型糖尿病;该核准案於1月27日通过,也就是欧盟核准该药物上市后隔天;自从胰岛素於1920年代被发现以来,吸入型粉状的基因重组人类胰岛素是第一个新的胰岛素投与选择;临床试验数据显示,使用exubera一开始会使肺功能下降,但是并非进行性的,其效果与一般胰岛素相当,而且具有病患顺从性较高的优点。

Therefore, we selected common species of Ranunculaceae. comparative primordial plants in Alpine Meadow at Eastern QinghaiTibetan Plateau, and studied the reproductive strategies, such as reproductive allocation, especially sex allocation, to understand how the plants adapt the environmental conditions. The results showed:(1) At higher altitude, the self-incompatible plant, such as Trollius ranunculoides tended to become limited by pollination rather than by nutrient availability, while the self-compatible plants, such as Anemone rivularis and A. obtusiloba seemed not to be limited by pollen availability, which suggested that ability of self pollination for alpine plants may be a mechanism of reproductive assurance.(2) Influences induced by high altitude on the reproduction of these species occurred on different reproductive characters, which suggest species-specific adaptive strategies. The influences are dependent on plant size to some extent.

因此,我们选择在青藏高原东部的高寒草甸,以比较原始的植物类群—毛茛科植物为研究对象,选择一些常见种探讨其繁殖对策,如繁殖分配和性分配问题,试图揭示这些植物如何适应环境条件,最后的结果表明: 1高寒草甸高海拔区,自交植物(如小花草玉梅A.rivularis和钝裂银莲花A.obtusiloba)的繁殖不受花粉限制,而完全异交植物(毛茛状金莲花T.ranunculoides)的繁殖受花粉限制,表明高海拔地区植物的白花授粉能力可能是一种繁殖保障机制,能够避免恶劣环境对植物有性繁殖的不利影响; 2海拔高度对植物的影响因种而异,不同的物种受影响的繁殖性状不同,即使同属亲缘种间也存在差异,表明不同物种对相似环境压力的适应对策不同;而且,海拔高度对植物的影响在一定程度上依赖于植物个体大小,说明植物通过调整其个体大小与繁殖性状的关系来适应环境梯度的变化; 3植物花期和果期的繁殖分配并不完全随海拔升高而减小(只有小花草玉梅的果期RA随海拔升高而减小30%),与Iwasa和Cohen的预测不完全一致(他们认为繁殖分配随生长季缩短而减小)。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

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