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性母细胞

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Methods 40 healthy adult mice were randomly divided into control group, CTX immune-suppressed group, Fb treated group, Fb combined with CTX treated group. The influences of Fb in NK cell activity were determined by the method of NK mediated cytotoxicity test;the multiplicative capacity of lymphocyte was assayed by lymphocyte transformation rate of T cell,and the mice spleen T lymphoblast multiplication analytical method was used to measure the IL-2 level.

健康成年小鼠40只,随机分为对照组、环磷酰胺免疫抑制组、黄蘑碱溶性多糖活性组分正常小鼠给药组和Fb环磷酰胺伍用给药组,采用NK细胞介导的细胞毒试验测定自然杀伤细胞的活性,淋巴细胞转化试验测定T淋巴细胞增殖功能,鼠脾T淋巴母细胞增殖分析法测定白细胞介素2(IL-2)的活性,观察Fb对各组小鼠免疫指标的影响。

The spiny eel , belonging to Perciformes, Osteichthyes, is a kind of little freshwater fish distributed in Southern China. In 1998, Our lab detected XX/XY differentiated sex chromosomes in this specie with karyotypic analysis, and then with chromosomal binding and SC observation, we identified that its two largest metacentric chromosomes were X, Y sex chromosomes in 1999. X, Y chromosomes differ each other not only in the distribution of heterochromatin but also in their shapes, which make them could be recognized directly under the light-microscope without any banding treatment.

刺鳅,属鲈形目、刺鳅科(Perciformes,Mastacembelidae),是广泛分布于中国南方的小型淡水鱼类。1998年,我室通过核型分析,首次发现该物种具有明显分化的异形性染色体,其性别决定类型为XX/XY型。1999年,本室通过染色体显带、精母细胞联会复合体观察,再次确认刺鳅染色体组中最大的一对中部着丝粒染色体为X、Y性染色体,两者不仅已经出现结构异染色质分布上的不同,同时在外部形态上也存在明显差异,识别特征鲜明,在光镜观察下无需显带即能清晰辨认性染色体。

All of these patients underwent plain and contrast-enhancement CT scan. Results: Thirteen cases included 5 neurilemmomas (3 malignant cases included), 5 neurofibromas, 1 neuroblastoma, 1 ganglioneuroma, 1 malignant paraganglioma. In the 13 cases, 2 cases derived from plexus sacralis, 2 cases located in the pelvic peritoneal space, 6 cases located in the pelvic extrapentoneal space, and 3 cases located in the vesica extraperitoneal space.

结果:神经鞘瘤5例(其中恶性3例),神经纤维瘤5例,神经母细胞瘤1例,节细胞性神经瘤1例,恶性副节细胞瘤1例。2例起源于骶丛,经由骶孔向盆腔内生长;2例起源于盆腔,其中1例源于膀胱,1例位于膀胱直肠陷窝;直肠腹膜外间隙6例;膀胱腹膜外间隙3例。

Two cases of MFL also showed polypoid or nodular. It was composed of centrocytic/centroblastic cells.(4) Large multilobated nuclei lymphomas were seen in two cases.(5)Histiocytic in three cases.

MFL 2例亦呈息肉样或形成肿块,由中心细胞和中心母细胞组成,(4)大多叶核淋巴瘤2例,(5)组织细胞性3例。

Immunohistochemically, cells in myoid/myofibroblastic areas showed positive staining for α-SMA, MSA and vimentin, but negative for desmin and CD34. Electron microscopic study displayed the presence of microfilament bundles, focal dense bodies and micropinocytic vesicles, consistent with those of myofibroblasts.

免疫组织化学标记显示肌样区域细胞表达α-平滑肌肌动蛋白和肌特异性肌动抗原,不表达CD34;电镜观察证实细胞含有质膜下微丝束、局灶性致密体及微胞饮囊泡样结构,与肌纤维母细胞相一致。

In contrast, 11/15 primary glioblastomas contained a significant CD133 subpopulation that displayed neurosphere-like, nonadherent growth and asymmetrical cell divisions yielding cells expressing markers characteristic for all three neural lineages.

相反,15个原代胶质母细胞瘤中有11个含有明显的CD133阳性亚群,呈神经球样,非附着性生长和非对称性细胞分裂,后者产生表达所有三种神经细胞系的特征标志物的细胞。15

Under low temperature condition, fertility restoring, anther developing normally, the tetrads formed after archesporical cell, sporogenous cells and microspore mother cell. Subsequently, microspores were free from the tetrad of fertile anther and formed mature pollen grains, tapetumof the fertile anthers began to disintegrate and disappear at last.

在低温处理条件下,育性恢复,花药发育正常,经过孢原细胞期、造孢细胞期、花粉母细胞期后,可以顺利形成四分体,可育花药的四分体可以释放出小孢子并发育为成熟的花粉粒,同时绒毡层细胞开始解体,最后消失。

ABSTRACT Objective To discuss the clinical pathologic features and differential diagnosis of mixed germ cell sex cord stromal tumorwith malignant variant of germ cell tumor Methods The clinical pathologic datum and immunophenotype were studied in the case of ovary MGCSCST with malignant variance of germ cell tumor Results The patient's genital anatomy and female phenotype were normal,karotypes was 46xx with uterogestation The tumor tissue consisted of mixed germ cells and sex cord stromal cells with evident malignancy of mixed germ cells There was no atypical structure of gonadoblastoma in tumor tissue Conclusion MGCSCSST has complex morphosis,thus,to prevent misdiagnosis,more samples should be collected and more slices be cut The patient may have prognosis malo when there are high malignant variants of mixed germ cells in tumor tissue

目的 探讨混合性生殖细胞性索间质肿瘤(mixed germ cell sex cord stromal tumor,MGCSCST)伴恶性生殖细胞肿瘤变异型的临床病理特点及鉴别诊断。方法卵巢MGCSCST伴恶性生殖细胞肿瘤变异型的病例进行临床病理特征和免疫表型分析。结果患者生殖器的解剖结构和女性表型正常,染色体组型是46xx,足月妊娠。肿瘤组织由混合性生殖细胞和性索间质肿瘤构成,混合性生殖细胞有显著的恶性特征。瘤组织中没有典型的性腺母细胞瘤结构。结论 MGCSCST有复杂的形态结构,因此更多的取材和切片可以防止误诊,当肿瘤组织中的混合性生殖细胞有高度恶性变异时,患者预后不良。卵巢肿瘤;生殖细胞性索间质肿瘤;免疫组织化学

The marrow between the pink bone trabeculae seen here is nearly 100% cellular, and it consists of leukemic cells of acute lymphocytic leukemia that have virtually replaced or suppressed normal hematopoiesis .

如图可见粉红色的骨小梁之间的骨髓几乎100%是细胞,是由急性淋巴母细胞性白血病的白血病细胞构成,这些细胞实际上替代或抑制了正常的造血作用。

At the same time,oocytes and spermatids were used as the samples to check the sensitivity of this nested PCR The results showed that this method can successfully check the templates of ten cells.

同时,以微量的卵母细胞和精子细胞为模板对该反应体系的灵敏性进行了检验,结果表明,该反应体系可对10个细胞水平进行准确鉴定。

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