性染色质

What was observed under a light microscope included: tumor cells were mulberry and micropapillary shaped or it was of glandule tubular arrangement; there was obvious interspace between cancer nest and neighboring areas; micropapillary was empty of fiber blood vessel axes; immunohistochemical staining showed ema positive location was both at outward surface of glandule duct and at micropapillary like cancer nest.

光镜下特征性表现为肿瘤细胞呈桑椹状、微乳头状或小腺管样排列，癌巢与周围间质形成明显的空隙。微乳头缺乏纤维血管轴心。免疫组化染色ema阳性部位在癌细胞巢团或微乳头状、腺管的外表面。

This is because neuroanl damage, reactive gliosis or loss of GFAP has not yet completed in such a short duration after an ischemic stroke.

因为在数小时内神经细胞死亡及胶质细胞增生之组织型态学以及GFAP 染色丧失或反应性增加之组织化学的变化皆尚未完成。

Histopathology of the hyperpigmented lesion revealed increase of melanin in the basal layer, pigment incontinence and amorphous eosinophilic masses stained positive as with Congo red in the papillary dermis.

色素沉著的病灶其组织病理显示在基底层有黑色素的增加，乳突状真皮出现色素失调和均质的嗜伊红性块状物质沉积，且以刚果红染色呈阳性反应。

Compared with control group,the ADM level in the left lung increased(P＜0.001),but the ADM levels in the heart,liver,kidney decreased.The ratio of wet to dry weight of the left lung in experiment group was 6.491±0.248,and 5.937±0.361 in control group,there was no significant difference（P＞0.05）.No alveolar wall thicken,alveolar and mesenchyma edema were found in two groups.

实验组兔左肺的湿/干重量比为6.491±0.248，右肺的湿/干重量比为5.937±0.361，双肺的湿/干重量比差异无显著性（P＞0.05）；HE染色结果显示实验组与对照组均未见肺泡壁增厚、间质及肺泡水肿等改变。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。