性功能

A/duck/Guangxi/22/2001 (DKGX/22) is nonpathogenic in mice, whereas A/duck/Guangxi/35/2001 (DKGX/35) is highly pathogenic.

我们用拮抗基因去产生一系列单一的综合基因，包括从DKGK/22中分离的一个及DKGX/35保留的7个基因片断，我们发现基因DKGK/22中的PA，NA，和NS某种程度能够消弱DKGX/35的致病性，但是DKGK/22病毒中的PB2能大大消弱DKGX/35病毒致病性，并且PB2的701位点天冬酰氨代替天冬氨酸在这种功能中起着主要作用。

Methods Peritoneal macrophages of guinea pigs were infected in vitro by three different Leptospira strains, the virulent Leptospira interrogans serovar Lai type strain Lai, the avirulent L. interrogans serovar Lai type strain IPAV, and the nonpathogenic L. biflexa serovar Patoc type strain PatocⅠ, respectively, and heat inactivated Staphylococcus epidermidis was added 0.5, 1.5, 3 and 6 h after infection and incubated for 30 min. The effect of Leptospira on the phagocytosis of macrophage was evaluated by the inactivated Staphylococcus epidermidis phagocytosis rate and phagocytosis index. Phagocytosis and ultrastructure of peritoneal macrophages were observed by transmission electron microscopy 3 h after infection, and changes of cytoskeleton of the macrophages were observed by laser scanning confocal microscopy.

用三种不同毒力的钩体（致病性问号钩端螺旋体赖型有毒株Lai株、赖型无毒株IPAV株以及非致病性双曲钩端螺旋体Patoc型PatocⅠ株）分别感染体外培养的豚鼠腹腔巨噬细胞，并分别于感染后0.5、1.5、3和6 h加入热灭活表皮葡萄球菌孵育30 min，通过计算巨噬细胞对灭活表皮葡萄球菌的吞噬率和吞噬指数，检测钩体对巨噬细胞吞噬功能的影响；感染后3 h透射电镜观察巨噬细胞对钩体的吞噬、降解和细胞超微结构的变化；用激光共聚焦显微镜观察细胞对钩体的吞噬和巨噬细胞细胞骨架的变化。

The following sections discuss orgasmic dysfunctions and their treatment in relation to women.

反过来，男性延后的性高潮罕有发生功能障碍，但是对於女性常常造成性不满足。

Objective:Postmenopausal Osteoporosis is syndrome shown on women caused by the functional descent on both ovaries and the level of estrogen. During the metabolism of their bones, the bone absorptive rate is higher than formative rate. As the result, the progression of calcium deficiency and the degeneration of bone tissue are the characters in this period. Clinical manifestations appear on these elder women, which are osteosclerosis and increasing the possibility of fracture.

目的： 绝经后骨质疏松症(Postmenopausal Osteoporosis，PMO)是指绝经后妇女由于卵巢功能衰退，雌激素水平下降，从而在骨代谢过程中，骨吸收大于骨形成，出现以低骨量和骨组织的显微结构的退行性变为其特征，临床表现为骨脆性和骨折易感性增加的一种代谢性疾病。

Objective: Menopausal osteoporosis is syndrome shown on women caused by thefunctional descent on both ovaries and the level of estrogen. During the metabolism oftheir bones, the bone absorptive rate is higher than formative rate. As the result, theprogression of calcium deficiency and the degeneration of bone tissue are thecharacters in this period. Clinical manifestations appear on these elder women, whichare osteosclerosis and increasing the possibility of fracture.

目的： 围绝经期骨质疏松症是指围绝经期女性由于卵巢功能衰退，雌激素水平下降，从而在骨代谢过程中，骨吸收大于骨形成，出现以低骨量和骨组织的显微结构退行性变为特征，临床表现为骨脆性和骨折易感性增加的一种代谢性疾病。

The network based on the ARM and the TCP/IP stack of uClinux provides the on-line GIS mo rnito ring system w ith stable communications and interchangeability. The ARM kernel in the embedded system p rovides a stable on2line monito ring system w ith real2time p rocessing capability and extendability.

应用测试结果表明：基于 ARM 和 uClinux 中TCPIP 协议栈的以太网通信，可以实现 GIS 在线监测系统的互操作性，并保证在通信功能上的长期稳定性；以 ARM 为核心的嵌入式系统硬件，可以保证监测装置的稳定性、实时性和可扩充性。

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能，本研究选择了MAP的主要保护性抗原Hsp65蛋白，以副结核分枝杆菌C-2株染色体DNA为模板，以hsp65基因的特异性引物进行PCR扩增，获得了1 626bp的hsp65基因，通过T-A克隆技术，将PCR产物克隆至pGEM-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pGEM-T-hsp65，以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列，结果表明，所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致，两者核苷酸序列的同源性为99.1％，氨基酸序列的同源性为99.3％，表明该基因在副结核分枝杆菌中高度保守。

The functions and features of the system are introduced. The questions such as adaptability, expansibility, transferability and perfectibility of the system are discussed.

对所建药物数据库系统的主要功能与特点，系统的适用性、可扩充性、可移植性以及如何使系统更加完善等问题进行了讨论。

Embedding artificial valve surrounding one side of external iliac vein of random selecting as experimental group, and the other side as control group. Using ascending phlebography and descending phlebography to check the function of artificial valve.

随机选择一侧髂总静脉安置人造瓣膜作为实验组，另一侧作为对照组，术后行上行性和下行性静脉造影检查人造瓣膜功能。

Meantime, the transparent ZnO electrode reduced the spatial separation between the liquid and the underlying electrode, and effectively photoelectric cooperative wetting occurred, with the surface changing from low adhesive to adhesive, which is propitious to develop in this field, such as the feasibility of the patterned photoelectric cooperative wetting, namely, applying a bias lower than the electrowetting threshold voltage, photoelectric cooperative wetting can be realized only at the illumination site, i.e., patterned site.

这一研究有利于拓展这一领域的发展，为开发新型的协同响应的功能浸润性界面材料提供新的思路。同时，浸润性从超疏水到亲水转变过程中，表面从低粘滞状态变为粘滞状态。这一过程为实现光电协同控制的图案化浸润提供了可能性，即在低于电浸润阈值电压的条件下，通过图案化的光照来实现液体图案化浸润。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。