念珠

At PCD3 and PCD7,the number of candidas was higher,but it decreased at PCD14.At PCD14,IL-4 expression in PP was the highest, while at PCD7 IL-6 expression in LP was the top one.

结果 白色念珠菌总数以定植3 d，7 d较高，14 d则明显下降；PP中IL-4表达以定植14 d最高；LP中IL-6阳性细胞数增加以定植7 d最高。

Methods Indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of Aspergillus fumigatus,respectively.Two different double monoclonal antibody sandwich ELISA assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of Aspergillus,Penicillium marneffei,and 5 species of Candidas.

采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心ELISA法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。

The ELISA assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of Aspergllius, while the other assay could only detect Aspergillus fumigatus of both clinical and environmental isolates.And no cross reaction with the cell culture of Penialllium marneffei and Candidas was observed with the two methods.

用mAbs-1建立的双抗体夹心ELISA法可检测19种常见曲霉株培养液；用特异性针对烟曲霉抗原单克降抗体(mAbs-2)建立的双抗体夹心ELISA法可特异性检测临床和环境分离株烟曲霉培养液；与其他曲霉株无交叉反应；2种双抗体夹心ELISA法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。

AIM: To prepare and identify the monoclonal antibody specific for the germ tube of Candida albicans.

目的制备抗白念珠菌芽管的单克隆抗体mAb03.2C1-C2，并对其特性进行分析，以探讨其应用于实验室检测的可行性。

Candida albicans ; germ tube ; monoclonal antibody ; characterization

白念珠菌；芽管；单克隆抗体；特性鉴定

Through database search, it was suggested that expression of REP5 could activate the expression of MUC1/FLO11, although the function of REP5 itself remained unclear. In this study, overexpression of REP5 did not significantly affect the drug susceptibility and germ tube formation of C. albicans.

文献报导REP5基因可以活化MUC1/FLO11的表现，虽然其自身功能尚不清楚，於白色念珠菌过量表现REP5基因时对於药物感受性和芽管形成并没有太大影响。

The result showed Indomethacin inhibited the formation of germ tube and hypha of candida albicans by dose dependence: indomethacin can inhibit 50% germ tube formation at 0.75mM level, while it can fully inhibit germ tube formation at 2.0mM level.

结果表明消炎痛对白色念珠菌芽管和菌丝形成的抑制作用与剂量相关：消炎痛浓度为0.75 mM时可抑制50%芽管形成，浓度为2.0mM时可完全抑制芽管和菌丝形成。

RPMI 1640 medium to induce hyphae at 37℃ for seven days was thought to be the favorable condition for C.

RPMI 1640培养基（P＝7.5），37aC传代培养7天（转种 12次），是获得菌丝相白念珠菌的理想条件； 3。

He told him of the red ibises, who stand in long rows on the banks of the Nile, and catch gold fish in their beaks; of the Sphinx, who is as old as the world itself and lives in the desert, and knows everything; of the merchants, who walk slowly by the side of their camels, and carry amber beads in their hands; of the King of the Mountains of the Moon, who is as black as ebony, and worships a large crystal; of the great green snake that sleeps in a palm-tree, and has twenty priests to feed it with honey-cakes; and of the pygmies who sail over a big lake on large flat leaves, and are always at war with the butterflies.

他还给王子讲那些红色的朱鹭，它们排成长长的一行站在尼罗河的岸边，用它们的尖嘴去捕捉金鱼；还讲到司芬克斯，它的岁数跟世界一样长久，住在沙漠中，通晓世间的一切；他讲到那些商人，跟著自己的驼队缓缓而行，手中摸著狼冶做的念珠；他讲到月亮山的国王，他皮肤黑得像乌木，崇拜一块巨大的水晶；他讲到那条睡在棕祸树上的绿色大蟒蛇，要二十个僧侣用蜜糖做的糕点来餵它；他又讲到那些小矮人，他们乘坐扁平的大树叶在湖泊中往来横渡，还老与蝴蝶发生战争。

He told him of the red ibises, who stand in long rows on the banks of the Nile, and catch gold-fish in their beaks; of the Sphinx, who is as old as the world itself, and lives in the desert, and knows everything; of the merchants, who walk slowly by the side of their camels, and carry amber beads in their hands; of the King of the Mountains of the Moon, who is as black as ebony, and worships a large crystal; of the great green snake that sleeps in a palm-tree, and has twenty priests to feed it with honey-cakes; and of the pygmies who sail over a big lake on large flat leaves, and are always at war with the butterflies.

他还给王子讲那些红色的朱鹭，它们排成长长的一行站在尼罗河的岸边，用它们的尖嘴去捕捉金鱼；还讲到司芬克斯，它的岁数跟世界一样长久，住在沙漠中，通晓世间的一切；他讲纽那些商人，跟着自己的驼队缓缓而行，手中摸着狼冶做的念珠；他讲到月亮山的国王，他皮肤黑得像乌木，崇拜一块巨大的水晶；他讲到那条睡在棕祸树上的绿色大莽蛇，要20个僧侣用蜜糖做的糕点来喂它；他又讲到那些小矮人，他们乘坐扁平的大树叶在湖泊中往来横渡

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失