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The data of children who were diagnosed as nosocomial candidaemia, were examined in this study.

研究分析了医院内诊断有念珠菌血症儿童的数据。

Background: Candida is an opportunistically pathogenic fungus.

背景:念珠菌是一种条件致病性真菌。

The most common microbes causing infections in percutaneously inserted uncuffed catheters are, in order of prevalence, coagulase-negative staphylococci, Staphylococcus aureus, Candida species, and enteric gram-negative bacilli.

根据患病率的高低,引起经皮插入的无气囊导管感染的最常见的微生物排序如下:凝固酶阴性葡萄球菌、金黄色葡萄球菌、念珠菌属菌种和肠道革兰氏阴性细菌。

On examination, the neonate had no evidence of mucosal and cutaneous candidiasis except yellowish discoloration and thickening of the fingernails and toenails with inflammation of the periungual area.

身体检查发现男婴的身上并没有黏膜或是皮肤念珠菌病。而手指甲及脚趾甲出现变黄变厚的现象,并且合并指甲周围发炎。

Lower leaves pinnatipartite, pinnatifid, or sinuate-dentate; fruit not torulose, with only stellate trichomes; subapical horns (2.5-)4-7(-8) mm, divaricate-reflexed, strongly recurved; valves extended beyond horns into stylelike apex 1-2 mm

下部叶羽状深裂,羽状半裂,或具深波状具牙齿;不近念珠状的果,具只星状毛;近尖端角(2.5-)4-7(-8)毫米,极叉开反折,强烈下弯;在进stylelike先端1-2毫米角状突起以外裂爿延伸 2 T。

Thirty specimens samples were randomly divided into three groups blank control,media control and inoculated m edia group, 10 specimens were used in each group under the aerobic conditions,Sa polos glucose agar media with candida albicans inoculated .

需氧环境将白色念珠菌接种到沙保罗葡萄糖琼脂培养基后,将试件贴附其表面每周转换传递1次,共10周。0.5g/L戊二醛消灭细菌清洗,2周后,用Minolta CR-100 型色度计观察。

Despite improvements of therapy, polymicrobial septicemia is increasingly being observed, and gram-negative bacteremia and candidemia represent significant causes of mortality in critically ill hospitalized patients.

尽管目前治疗感染的措施已有很大提高,但多种微生物混合感染的发病率仍在逐渐增多,革兰阴性菌和白色念珠菌感染仍然是住院危重病人的重要致死原因。

Among the macroporous resin fractions of 30%, 50%, 70%, 80% and 95% ethanol, the 70% ethanol fraction showed the highest antifungal activity towards Schizosaccharomyces pombe and Candida albicans.

在30%,50%,70%,80%,95%乙醇溶液的大孔树脂柱层析洗脱组分中,70%乙醇溶液洗脱组分对裂殖酵母菌和白色念珠菌的抗真菌活性最强。

The male prosopon body is tall and slender,is smaller than the female prosopon,0.6-3.0mm,the antenna growth is approximately good,reads beaded,the garnet,has the little white wax powder.

雄成虫体细长,比雌成虫小,约0.6—3.0mm,触角发育良好,念珠状,暗红色,有少许白色蜡粉。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育,MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用;RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1),以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况;Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达,以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用;免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达;激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位,并观察真菌刺激后人角膜上皮细胞骨架的变化;流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变;光学显微镜观察真菌与人角膜上皮细胞黏附数量,记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后,细胞超微结构的改变。

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