念珠

Keratin antibody IgM 3B4. CONCLUSION: Natural monoclonal anti??keratin antibody IgM 3B4 can inhibit the adhesion of Candida albicans to the malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein, and decrease the invasion of Candida albicans due to its inhibitory action on the formation of Candida albicans germ tube,which indicates that natural antibody IgM plays a role in the natural immunity against yeast infection.

单克隆天然抗角蛋白抗体IgM 3B4在体外能够抑制白念珠菌芽管形成，能够抑制白念珠菌与人角质形成细胞、人口腔黏膜上皮细胞、胎儿脐静脉内皮细胞的粘附，从而降低白念珠菌的侵袭力，提示天然抗体IgM在机体抗真菌感染的天然免疫中可能具有积极的作用。

And the different concentrations of natural monoclonal anti-keratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal anti-keratin antibody IgM 3B4 to the adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein.

结果：与对照组比较，不同浓度的单克隆天然抗角蛋白抗体IgM3B4均能抑制白念珠菌芽管生成，并且抗体浓度越高，抑制白念珠菌芽管形成的作用越明显；同时单克隆天然抗角蛋白抗体IgM3B4能够显著减少白念珠菌与角质形成细胞、上皮细胞、内皮细胞的粘附，其抑制作用与单克隆天然抗角蛋白抗体IgM3B4的浓度呈正相关。

Keratin antibody IgM 3B4 to the adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein. RESULTS: It was found that the different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 could inhibit Candida albicans germ tube formation and adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein.Its inhibitory action was positively related to the level of natural monoclonal anti??

结果：与对照组比较，不同浓度的单克隆天然抗角蛋白抗体IgM 3B4均能抑制白念珠菌芽管生成，并且抗体浓度越高，抑制白念珠菌芽管形成的作用越明显；同时单克隆天然抗角蛋白抗体IgM 3B4能够显著减少白念珠菌与角质形成细胞、上皮细胞、内皮细胞的粘附，其抑制作用与单克隆天然抗角蛋白抗体IgM 3B4的浓度呈正相关。

Results: Of the 70 cases, Monilia albicans accounted for 61.43%, slick Monilia 21.43%, Candida tropicalis 11.43 and Candida Krusei 5.71%.

结果：念珠菌感染70例中，白色念珠菌占61.43%，光滑念珠菌占21.43%，热带念株菌占11.43%，克柔念株菌占5.71%。

The signals look like noncontinuous beads on the DNA fiber,which consist of multi-copy and arrange tandemly.Fiber-FISH results with 45S rDNA probe showed that an average signal length is about 3～11μm in many tandem copy sequence(measure number,n=8),so we estimated the size of each copy to be approximately 11～30kb in cotton genome.There are dual signals on each end of the mitosis metaphase chromosomes and pachytene chromosomes hybridized with telomere DNA probe.The signals also look like non-continuous beads on the DNA fiber,the length is about 1～9μm,so we estimated the size is about 4～27kb.The signals almost covered the whole mitosis metaphase chromosomes and pachytene chromosomes of G.arboretum Shixiya 1 hybridized with gDNA probe,the euchromatin zone and heterochromatin zone were identified clearly on pachytene chromosomes,and the signals also look like non-continuous beads.Two BAC clones 150-D-24 and 182-F-9 in DNA BAC library of Pima90 were selected as probe to hybridize with mitosis metaphase chromosomes,pachytene chromosomes and DNA fiber of G.raimondii.

棉花FISH技术系统的应用研究。45S rDNA在亚洲棉石系亚1号中期和粗线期染色体上有两对大的信号，在DNA纤维上信号为非连续的念珠状，多个拷贝串联排列，每个拷贝长度大约在3～11μm，推测每个拷贝的实际长度为11～30kb；端粒序列在亚洲棉石系亚1号中期和粗线期染色体端部都有双点信号，在DNA纤维上信号也为非连续的念珠状，不同染色体上信号长度大约在1～9μm不等，推测实际长度为4～27kb；gDNA信号几乎布满整个亚洲棉石系亚1号中期和粗线期染色体，在粗线期染色体上能明显区分出常染色质区和异染色质区，DNA纤维上的信号均为非连续的念珠状。

Albicans strains with fluconazole. Method: A series of 16 strains C. albicans from the same body were induced to form pseudo-hyphae in RPMI 1640 medium and DMEM medium at 37 癈 for twenty-four hours respectively. The pseudo-hyphae and yeast were counted and the ratio between pseudo-hypha and total cells was calculated. C. albicans strains were induced to form pseudo-hyphae in RPMI 1640 medium for seven days by transferring twelve times. The genome DNAs of the hyphal-form of C. albicans was cleaved by two restriction enzymes EcoR I and Bgl II, the products were electrophored and photoed.

以同一母体来源的白念珠菌CA-1～A-17(共16株)作为研究对象，采用RPMI1640和DMEM培养基，37℃培养24h诱导其假菌丝形成，记录不同观察时间时的菌丝相和酵母相细胞数，计算菌丝相白念珠菌形成率；以RPMI1640培养基，37℃连续传代培养7天(转种12次)后，分离菌丝相白念珠菌并提取其基因组DNA；以EcoRⅠ和BglⅡ消化菌丝相基因组DNA，消化产物进行琼脂糖凝胶电泳并拍照；用随机引物(5'…TCACCACGGT…3')扩增菌丝相基因组DNA，采用变性聚丙烯酰胺凝胶电泳分离扩增产物并拍照。

Results In this 323 strains of fungus, candida albicans was the main species, according for 63.8％, candidas tropicalis for 22.3％, and candidasglabrata for 7.4％.The resistance rate to 5 antifungals was ordinalhere:5FC 1.9％, AP 2.2％, KE 5.6％, IT 10.2％, FL 12.1％.

结果：323株真菌中，白色念珠菌、热带念珠菌、光滑念珠菌等分别占63.8%、22.3%和7.4%；对5种常用抗真菌药的耐药率排序依次为：5氟胞嘧啶1.9%、****霉素B2.2%、酮康唑5.6%、依曲康唑10.2%、氟康唑12.1%。

Results In this 323 strains of fungus, candida albicans was the main species, according for 63.8％, candidas tropicalis for 22.3％, and candidasglabrata for 7.4％.The resistance rate to 5 antifungals was ordinalhere:5FC 1.9％, AP 2.2％, KE 5.6％, IT 10.2％, FL 12.1％.

结果：323株真菌中，白色念珠菌、热带念珠菌、光滑念珠菌等分别占63.8%、22.3%和7.4%；对5种常用抗真菌药的耐药率排序依次为：5氟胞嘧啶1.9%、两性霉素B2.2%、酮康唑5.6%、依曲康唑10.2%、氟康唑12.1%。

The protection of the mAb was analyzed via the inhibition of the mAb to the germ tube formation and the adhesion of C.albicans to the epithelial and endothelial cells.

在白念珠菌芽管形成条件下，加入mAb，观察其对白念珠菌芽管诱导的抑制及白念珠菌对上皮细胞、内皮细胞黏附的抑制。

The highest susceptibility rate of candida to six kinds of anti-candida drug was AMB and Nystatin...

慢性前列腺炎中念珠菌感染率为 11.5 %，主要为白念珠菌引起(占 6.4%)；AMB、NYS为念珠菌最敏感药。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失