必须的

Mind must adhere to the guidance of scientific concept of development, the overall situation in mind, heart employees; must have advanced awareness and strategic thinking; must oppose bookishness and dogmatism; must get rid of thinking that nothing thinking; must have for the cause of responsible for the courage to sacrifice and the spirit of courage.

解放思想必须坚持以科学发展观为指导，胸怀全局，心系职工；必须有超前意识和战略思维；必须反对本本主义和教条主义；必须破除安于现状、不思进取的思想；必须具备为事业敢于牺牲和敢于负责的精神。

Laurence Cummings, the musical director of the London Handel Festival, suggests multiple causes: more research into period performance that has led to more historically informed playing with smaller musical forces and a clearer, purer sound; the rise of the countertenor, the modern equivalent of the castrato without the unacceptable job requirements; even the growth of the recording industry, always on the lookout for new gems.

劳伦斯·卡名斯，伦敦韩德尔音乐节的音乐总监，道出了其中多方面的原因：对不同时期表演的深入研究导致了更多虽然音乐表现力较弱，但有着更纯粹声音的兼具历史特征的作品的出现；上男高音的崛起代替了原来的阉唱男高音，而且上男高音还不必接受阉唱男高音所必须的令人难以忍受的职业要求；甚至不断成长的音像工业也在一直寻求着新的音乐珍宝。

We have to deal with everyday existence; we have to act, we have to legislate, we have to govern and bring order out of chaos.

我们必须应付每一天的存在，我们必须行动，我们必须立法，我们必须管理，为混乱带来秩序。

Idea of freedom 自由的概念 In the Critique of Pure Reason, Kant distinguishes between the transcendental idea of freedom, which as a psychological concept is "mainly empirical" and refers to "the question whether we must admit a power of spontaneously beginning a series of successive things or states" as a real ground of necessity in regard to causality, and the practical concept of freedom as the independence of our will from the "coercion" or necessitation through sensuous impulses.

在《纯粹理性批判》中，康德区分了先验自由的概念与实践自由的概念，先验自由概念作为一个心理学概念"主要是经验的"，它指向"我们是否必须承认自然始发的一系列先后相继的事物状态"是必然性的真正基础的问题；实践自由的概念则是作为我们的意志独立于"强迫"或"通过感官冲动的强制"的概念。

Iii "Cost of restoration and repair" includes all actual and projected costs of curation, disposition, and appropriate reburial of, and consultation with respect to, the cultural heritage resource; and any other actual and projected costs to complete restoration and repair of the cultural heritage resource, including its reconstruction and stabilization; reconstruction and stabilization of ground contour and surface; research necessary to conduct reconstruction and stabilization; the construction of physical barriers and other protective devices; examination and analysis of the cultural heritage resource as part of efforts to salvage remaining information about the resource; and preparation of reports.

"复原和修复成本"包括所有实际的和将发生的修补、安置和适当的埋藏文化遗产资源，并与有关方进行协商成本；所有实际的和将发生的完成复原、修复文化遗产资源的成本，其包括重建或加固成本；重建或加固围路曲面和表面成本；进行重建或加固所必须的研究成本；建设栅栏或其他保护设施的成本；作为抢救文化遗产资源残余部分努力之一的检查和分析文化遗产资源成本；撰写报告的成本。

If you want to be a 1st class Kung Fu player, you have to suffer a lot, you have to practice, you have to learn, you have to be good people. You have to have the spirit of never give up, the fighting spirit, keep on doing.

你必须要经历很多事情，你必须去实践，你必须去学，你必须要成为一个好人，你必须要有一个永不放弃的精神，战斗的精神，保持有所作为。

Using cytochalasin D to prevent global actin from forming filamentous structures, the production of Heliothis armigera nuclear polyhedrosis virus in Hz-AM1 cells grown in the medium containing 0.5μg/ml CD was completely inhibited while its production was reduced to 10〓 in the presence of 0.1μg/ml CD by plaque assay. However, Western blotting revealed that the actin quantity in infected host cells treated or untreated with CD was almost identical. Additionally, CD had no effect on the DNA synthesis of HaNPV.

Western blot表明细胞松弛素D并不影响受染细胞中肌动蛋白的含量，Dot blot表明病毒DNA的合成也没有受到影响，但电子显微镜超薄切片发现在0.5ug／mL细胞松弛素D中形成的病毒粒子形态与正常的有明显区别，认为细胞松弛素D抑制棉铃虫核型多角体病毒的增殖是因为抑制病毒的DNA和蛋白组装成完整有感染性的病毒粒子，即丝状肌动蛋白对病毒粒子的脱壳和DNA的复制、转录没有影响，但对病毒粒子的组装是必须的。

In this thesis, we find that dlg is indispensible in the establishment of anterior-posterior and dorsal-ventral polarity of drosophila oocyte. Removal of Dlg function from the posterior follicle cells using the FLP/FRT system leads to disruption of oocyte skeleton reconstruction that is elicited by the failure of those posterior cells to differentiate normally in mid-oogenesis. We demonstrate that abnormity of Notch, JAK-STAT and EGFR signal pathway in dlg mutants contributes to this aberrant differentiation. dlg null mutant also blocks the normal differentiation of two groups of anterior follicle cell-stretched cell and centripetal cell, but not border cell, with a lower penetrance. However unlike the result in posterior follicle cells, Notch and JAK-STAT signaling are both undisrupted in all mutant anterior follicle cells, implying other fate determinants may be involved.

我们的研究发现，后端滤泡细胞中的Dlg在果蝇卵子发生中期卵母细胞前后轴和背腹轴建立过程中也是必须的，PFC中dlg完全缺失型突变引起PFC的分化异常，导致卵子发生中期卵母细胞骨架重组异常，Stau、Grk等极性决定蛋白定位错误。dlg突变阻碍了Notch、JAK-STAT、EGFR等调节PFC分化的信号通路的激活。dlg突变的PFC也没有获得前端滤泡细胞命运。dlg突变不影响前端滤泡细胞群中边界细胞的分化，但是在一定程度上影响伸展细胞和向心细胞的分化，并且这种影响不依赖于前端滤泡细胞Notch或JAK-STAT信号激活的异常。

The equity argument is, of course, inspired by the fact that the threatening consequences of past natural resource development in and by industrialized countries are now being invoked as the reason for preempting many Third World nations from exercising their ' natural development options. Finally, developed countries .night experience difficulties in mustering the requisite political will to offset the potentially huge costs that developing nations are likely to incur by complying with internationally advocated restraints on domestic development strategies. Indeed, nothing short of an unprecedented international redistribution of wealth might be necessary to secure the full co-operation of developing countries in countering the threat of global climate change.

当然，尽管事实是过去国有资源的开发是工业化国家强迫的结果，现在却祈求把保护环境作为一种原因阻止组织第三世界国家开发他们的国有资源引起了这种公平的争执，最后，发达国家可能要担负必须的政治上的困难去弥补潜在的发展中国家由于接受国际上提倡者的抑制民族工业发展战略所带来的巨大花费，的确，除非国际财富作空前的必要的分配去解决由于发展中国家的联合反对而带来的全球气候变化的威胁。

Please read this document carefully and report the observations in the correct format noting that the UTC time of observation should be reported to 0.00001 day, the R.A.

请仔细阅读该文件并按照正确的格式上报（注意，时间必须精确到0.00001天，赤经必须精确到0s.01，赤纬必须精确到0.1，星等必须精确到0.1等）。

This paper discusses design and realizable methods of remote test output interface from logical design angle.

本文从逻辑设计的角度讨论遥测输出接口的设计及实现方法。

This also 星体投射plies to buildings, structures and geological features.

这也适用于建筑物和地质特征。