必需

The customer service is an extremely challenging job, I like very much, however, I must give up first now, because in case of a chance, receive a foreign guest's telephone, that is only a section of ordinary dialogues, I speak intermittently, ambiguously, let me realize a question, but that but my level is too low, I must go to receive a training!

客服是一个极具挑战性的工作，我很喜欢，不过，现在我必需先放弃，因为在一次偶然的情况下，接到一位外国客人的电话，那只是一段普通的对话，我却说得断断续续，含糊不清，让我意识到一个问题，那就是我的水平太低了，我必需去进修！

Payment : The payment should be by Irrevocable, Confirmed, Transferable, Documentary Letter of Credit that acceptable by the Seller's bank with 12 months validity. Buyer should made the payment for each shipment within 7 banking days after the goods arriver at discharging port with CIQ and SGS report and within 3 banking day by direct telegraphic transfer to the Seller's account against shipping documents.

付款方法：付款方式以不可撤销，可确认，可转让及卖方银行接受的跟单信用证作付款担保工具，信用证有效期为个月，买方每船期付款必需在收到船务文件及货品抵达卸货港后七天内必需完成中国商检及SGS商检报告后于3个银行工作天内直接电汇至卖方户口。

Copper is one of the essential microelements for plants. But in recent years, the abuse of copper bactericide and industrial pollution has led to the increase of copper content in environment such as in soil.

铜是一种高等植物生长发育过程中必需的重要微量营养元素，是许多胞内酶所必需的组分或辅酶，它参与很多生理代谢过程，对作物的发育、品质、产量等有重要的影响。

To better understand the roles of LsrA protein in nodulation, a series of analyses of nodules using scanning electron microscopy, genetic and biochemical approaches have been carried out. Our analyses suggest that the lsrA1 nodules contain bacteroids in the invasion and establishing zone only. The lsrA gene expression is active early in the invasion zone and activated later than bacA genes. The lost of LsrA protein dramatically reduced the expression of nifA and fixK, and completely blocked the expression of the nifH gene for nitrogenase. LsrA protein functions early in the bacteroid development and it is essential for the development nitrogen fixing bacteroids.

前期的工作中，苜蓿中华根瘤菌Rm1021中90个候选LysR基因已经被定向插入突变，并筛选在自生生长时期、共生生长时期的表型，以期寻找更多在自生状态或共生固氮中有功能的LysR转录因子。1 针对前期鉴定出的共生固氮必需的lsrA基因，我们应用了一系列扫描电子显微镜技术、生物化学、分子遗传学等方法，发现lsrA基因主要在根瘤侵染区开始表达，表达时序也在侵染阶段左右，但晚于bacA基因表达；LsrA蛋白缺失后根瘤固氮区中缺乏具有固氮能力的类菌体，nifA和fixK基因的转录水平降低，nifH基因的转录被完全阻断，因此LsrA蛋白为根瘤发育所必需，是新的根瘤发育信号传导途径成员。2 通过表型筛选我们鉴定了苜蓿中华根瘤菌的oxyR基因，并研究了它的调节特性。oxyR突变后，苜蓿中华根瘤菌对过氧化氢敏感性提高，适应性降低。

Though the composition of the amino acids was not balanced,the contents were among that of bean cake and bone-flesh powder.

虽然必需氨基酸组成不平衡，但必需氨基酸含量介于豆饼和肉骨粉之间。

The differences of 7 kinds of essential amino acid content for people,11 kinds of essential amino acid content for pig,14 kinds of essential amino acid content for chook,amino acid content,grain yield,protein-nitrogen content and protein yield of brown rice between forage rice and eatable rice were significant or extremely significant.

饲料稻品种糙米中人体7种必需氨基酸含量、猪11种必需氨基酸含量、鸡14种必需氨基酸含量、氨基酸含量、籽粒产量、糙米蛋白氮含量和糙米蛋白质产量与食用稻品种之间差异显著或极显著。

Your alignment must match your deity's; an invoker of a good deity must be good, an invoker of a lawful good deity must be lawful good, and an invoker of an unaligned deity must be unaligned.

你 的阵营必需与你的神相同：善神的神导士必需是善良，守序神的神导士必需是守序善良，而无阵营的神的神导士也必需是无阵营。

The playback method includes a first step of outputting stereo audio signals using the monaural audio signal if the necessary stereo process information is not supplied; a second step of starting updating stereo variables within filters, and outputting the stereo audio signals using the monaural audio signal until all the state variables are updated, if the necessary stereo process information is supplied; and a third step of performing the stereo process based on stereo process information acquired by the necessary stereo process information, on the monaural audio signal to generate and output stereo audio signals, if all the state variables within the filters are updated.

该再生方法包括：第一步骤，如果没有提供立体声处理必需信息，则输出使用了非立体声音频信号的立体声音频信号；第二步骤，如果提供了立体声处理必需信息，则开始更新滤波器内的状态变量，并输出使用了非立体声音频信号的立体声音频信号，直到更新完所有的状态变量；以及第三步骤，如果更新完滤波器内的所述状态变量，则对非立体声音频信号执行基于通过立体声处理必需信息获取的立体声处理信息的立体声处理，并生成并输出立体声音频信号。

To open and consolidate the revolutionary base needs the cultural publicity work and as well the regional ruling.

根据地的开辟与巩固，必需文化宣传工作，区域性的执政需求亦必然如此；根据地发展的可持续可待久，必需开展社会改造和社会重建，必需重视教育事业，传播先进的文化文明理念；促进社会进步，既是中国共产党的奋斗目标，也是红军的奋斗目标。

The results showed that the crude protein content,the eighteen essential amino acids and the twelve essential amino acids in the powder of tetraploidy black locust for fodder leaves were highly at the four stages,and along with the time process,they were all descending gradually.Moreover,their essential amino acid score were also highly and their amino acid were balanceable.And their essential amino acid index of cattle,pig,chicken,duck,goose,fish,prawn and sheep were higher than 0.86,therefore they can be the excellent fodder of these animals.

结果表明，饲料型四倍体刺槐叶粉4个时期的粗蛋白1、8种氨基酸总量和12种必需氨基酸含量均较高，且呈现随时间的推移而逐渐下降的趋势；其必需氨基酸分均较高，氨基酸组成较平衡；对牛、猪、鸡、鸭、鹅、草鱼、对虾和羊8种动物的必需氨基酸指数4个时期之间无差异，均大于0.86，可作为这些动物的优良植物饲料蛋白源；在7月中下旬其生物量和营养达到了最佳结合点，适宜采摘或刈割。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。