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In the present study, we investigate the effect of acidification and alkalinization on primary culture of rat cardiomyocytes.

在本研究中,我们以较广泛的角度,观察初代培养的心肌细胞内的酸化或是碱化对於细胞产生死亡机制的影响。

METHODS We used a dermatome to obtain thin endocardium,midcardium and epicardium transmural strips from left ventricular free wall of canine(weight 15-18 kg) and standard microelectrode techniques to record and measure the transmembrane action potential duration.

结果:①胺碘酮延长内、外膜层心肌细胞的动作电位时程,当基础周长为1 000 m s时,使用胺碘酮后心内膜层肌细胞的APD90由(254±38)m s延长为(274±37)m s(P.01),外膜层肌细胞的APD90由(205±37)m s延长为(229±16)m s(P.01);但其明显缩短中层肌细胞的APD,其APD90由(331±50)m s缩短为(290±62)m s(P.01)。

AIM: To observe the effect of endoxin antagonist,anti-digoxin antiserum,on endoxin level,ATPase activities,intramitochondrial total calcium concentration and gene expression of sodium pump isoforms in myocardium of rats with myocardial ischemia reperfusion.

目的: 观察内洋地黄素特异性拮抗剂地高辛抗血清对心肌缺血再灌注损伤大鼠心肌组织内洋地黄素水平、钠泵活性、线粒体总钙浓度以及钠泵各亚基基因表达的影响,探讨内洋地黄素在心肌缺血再灌注损伤中的作用及其机制。

The activity of superoxide dismutase in the myocardial cells was determined by the diphenol method, plasma malondialdehyde content was measured by TBA assay, the culture medium LDH content was measured by the acetonic acid method, the intracellular calcium concentration was measured by fluorescence spectropho- tometer- The results was shown that aspartate-K.

结果显示,门冬氨酸钾镁能减少阿霉素中毒心肌细胞乳酸脱氢酶的释放量,降低细胞内丙二醛含量及游离钙浓度,同时可升高细胞内超氧化物歧化酶活力;结果表明,门冬氨酸钾镁可能通过抗脂质过氧化及逆转细胞内钙离子过负荷作用进而保护中毒心肌细胞。

A total of 90 healthy male Sprague Dawley rats were selected for this study. Twelve rats were used for the sham operation group. Left anterior descending coronary artery of the remaining rats was deligated to establish models of myocardial infarction. After 24 hours of deligation, 36 rats were randomly divided into the control group and experimental group, 18 rat in each group.

目的:检测人胰岛素样生长因子Ⅰ基因修饰的鼠骨骼肌成肌细胞移植后,心肌梗死大鼠血浆及心肌组织内胰岛素样生长因子Ⅰ蛋白浓度的变化,以及心肌细胞内胰岛素样生长因子Ⅰ和端粒酶反转录酶mRNA水平的表达。

There were no significant changes in Na〓, K〓, and Cl〓 plasma concentrations. The results confirmed the feasibility of intramyocardial injection of hANF gene and contributed to next study in hANF gene expression by intracoronary transduction.

这一结果也进一步证实了直接心肌内注射hANF基因之导入方法的有效性及可行性,同时也为hANF基因冠状动脉导入的研究奠定了良好的基础。

Others injected MSCs into autoallergic myocardial tissue directly, observed some caridomyocyto-like cells formation capable of cardiomyocyte specific marking of troponin T and myoglobulin heavy-chain positive staining, which proved that MSCs can be induced into myocardial cells in microenviroment in vivo.

另有学者将MSCs直接注入自体心肌组织内,发现有心肌样细胞形成,具有肌钙蛋白T和肌球蛋白重链染色阳性的心肌细胞特异性标志,表明MSCs在体内微环境条件下可以诱导分化为心肌细胞。

Several investigators have determined that placing the electrode parallel rather than perpendicular to the target nerve substantially increases the size of the lesion, thereby reducing the likelihood the treatment will miss or only partially coagulate the target nerve.234,235 After a literature review and cadaveric study, Lau et al.234 concluded the ideal electrode position is across the lateral neck of the superior articular process rather than the groove formed at the angle of the superior articular and transverse processes, as was used in most studies.159,160Other investigators have found the maximal lesion size to be reached within 60 s of lesion time,235–237 independent of whether the system is temperature or voltage controlled.238 Studies conducted in human myocardium have determined that irrigation fluid has either no effect or a slightly beneficial effect on lesion size.239 Hence, the use of LA to prevent procedure-related pain or steroid to reduce the incidence of neuritis184 should theoretically have no adverse effects on the efficacy of radiofrequency denervation.

一些研究者认为,将点击平行的置于目标神经比将其垂直置于目标神经增加了消融的范围,因而减少损伤可能使目标神经不被或部分被消融。Lau等回顾了文献中对尸体的研究,总结出理想的电极放置位置为上关节突颈部外侧而非常采用的上关节突与横突形成的沟。其他学者研究发现,不论系统是温度控制或电压控制,其最大的损伤范围都在60秒内达到。在心肌灌注的研究中,灌注液多损伤范围造成轻微或不造成损伤。因此,使用LA预防过程相关性疼痛或类固醇防止神经炎发生时,需理论上确保对射频消融效果无副作用。

Results: Compared with control dogs, sections from AF dogs showed cellular hypertrophy, disarranged cell bundles and increasing connective tissue.

结果:与对照犬相比,AF犬肺静脉肌袖内心肌细胞增大、排列紊乱;细胞核大小不甚规则,核异型性明显,细胞内可见肌纤维断裂;心肌纤维之间连接组织积聚,使心肌细胞之间的间隔增宽。

Beating heart during openheart surgery is a good method to protect the myocardium being close to physiological condition, and the protective effect may be attributed to alleviation of ischemia/reperfusion injury.

浅低温体外循环心脏跳动中心内直视手术是一种较接近生理状态的心肌保护方法,能最大程度地减轻心肌缺血、缺氧损伤,避免再灌注损伤,最大程度地保持心肌细胞形态结构的完整性,从而获得较理想的心肌保护效果。

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