心肌内的

Results (1)The permillage of myocyte apoptosis in infarct area in elder patients with sudden death caused by AMI was significantly higher than that in control group(663.00‰±117.12‰ vs 34.30‰±20.68‰,P＜0 .01).However the permillage of myocyte apoptosis in 1-vessel,2-vessel,and 3 - vessel disease were 514.28±165.12‰,564.38‰±102.33‰ and 668.25‰±127. 19‰,respectiverly,significantly higher as compared to control group(All P＜ 0.01).But no significance was found among the three groups.(2)The size of DNA f ragment about 180～200 bp was found only in those patients with two and three ve ssels involoved.(3)The electron microscope showed the characteristics of myocyte apoptosis episodes,the others showed the characteristics of necrosis.

结果 TUNEL法发现，猝死者梗死区的心肌细胞凋亡千分数老年组(663.00‰±117.12‰)明显高于正常对照(34.30‰±20.68‰)(P＜0.001)；心肌细胞凋亡千分数在冠脉1支病变者(514.28‰±165.12‰)＜2支病变者(564.38‰±102.12‰)＜3支病变者(668.25‰±127.19‰)，虽然均明显高于正常对照(均P＜0.01)，但三组之间比较则无统计学上的差别(均P＞0.05)；冠脉2～3支病变者梗死区的心肌细胞DNA电泳可见相差约180～220 bp的阶梯片段；电镜发现猝死者梗死区内的心肌细胞核膜完整、染色质浓集、电子密度增加的凋亡特征，有的则出现核膜破裂、染色质溶解成碎屑的坏死现象。

Objective: To investigate the effect of intramyocardial bone marrow cell injection on myocardial perfusion and LV function in patients with chronic myocardial ischemia.

目的：研究骨髓干细胞心肌内注射对慢性心肌缺血患者心肌灌注及左室功能的影响。

Objective:To study intramyocardial flow characteristics in patients with coronary artery disease by color Doppler coronary flow imagingand to assess clinical value of CDCFI.

目的：应用冠脉血流显像技术评价冠心病患者的心肌内血流特征，探讨该技术在检测心肌内血流方面的价值。

In recent animal studies intramyocardial transplantation of ESCs or their differentiated cardiac-like cells regenerated injured myocardium and improved heart function in infarcted animal models.

新近的研究表明，在心肌梗塞动物模型中，心肌内移植胚胎干细胞或由其分化成的心肌样细胞，能导致已损伤心肌的再生，并改善心脏功能。

intramyocardial microvasculopathy on the early stage of diabetic patients may affect the flow dynamics of intramyocardial microvascular, and lead to myocardial ischemia impairments when no any coronary pathological changes exist in diabetic patients.

糖尿病早期心肌微血管的病变使心肌内冠脉血流动力学发生了变化，造成糖尿病心肌在无冠脉病变时的缺血损伤。

Part three: Hemodynamic changes of intramyocardial microvascular by ultrasound in diabetic patients Objectives: To observe and analyze the hemodynamic changes of intramyocardial microvascular which is caused by intramyocardial microvasculopathy in diabetic patients using TTDE. Methods: 27 normal subjects and 50 patients were studied.

第三部分糖尿病患者心肌内冠状动脉的血流动力学变化目的：应用冠脉血流显像技术观察糖尿病患者静息状态下心肌内冠脉分支血流，分析糖尿病患者发生的冠状动脉微血管病变带来的血流动力学改变。

A coronary microthromhoembolism model was successfully established by injecting sodium laurate into the coronary arteries. Coronary micro-thrombogenesis may promote the apoptosis of cardiomyocytes, and is one of the reason for heart function decrease. Telmisartan can efficiently suppress ventricles reconstruction, improve heart function, and inhibit the apoptosis of myocardial cells after microthrombogenesis in coronary artery.

冠状动脉内注射月桂酸钠能够成功建立冠状动脉微血栓的模型；冠脉内微血栓增加心肌细胞的凋亡，是导致心功能下降的原因之一；替米沙坦能有效地抑制心脏重塑，改善心功能，并能抑制冠脉微血栓后心肌细胞的凋亡。

Exercise testing may explore myocardial blood perfusion,nuclein myocardial imaging may accurately explore range and extent of myocardial ischemia and predicate coronary restenosis for patients with coronary stenting.

对于冠状动脉内支架植入术后病人进行运动负荷试验可了解患者的心肌血流灌注状况，核素心肌显像能准确提供心肌缺血的范围和程度，可预测血管的再狭窄。

To test the hypothesis, neontal rat cadiomyocytes Treat with heat shock(HS,42℃,2h) to induce the expression of HSP70,then teated with 0.5mmol/L H2O2. We first found H2O2 can reduced cadiomyocytes apoptosis and HSP70 only over-expression in the HS group, other two group only express HSP70 a little in the test of expression of HSP70.Then we tested the concentration of Ca2+, the [Ca2+ ] of CON was 192.224+6.654, the [Ca2+ ] of H2O2 group was 290.6918+8.922and the [Ca2+]of HS group was 214.2633+4.484.To further determine how HSP70 repaired the Ca2+ homestasis, we measured calcium transient of each group.

实验中应用MTT和流式细胞技术检测细胞的凋亡，发现损伤组的存活率明显低于其他2组，热休克组(Heat shock group，HS group)细胞存活率略低于正常组细胞；免疫组化结果表明只有在HS组才检测到阳性反应，即是HSP70的表达，其他2组均为弱阳性结果；应用离子成像技术对细胞内钙离子浓度进行测定，发现损伤组290.6918+8.922明显高于正常组192.224+6.654和HS组214.2633+4.484，HS组细胞内的钙离子浓度略高于正常组；为了进一步探讨HSP70对于ROS引起的心肌凋亡过程中钙离子的调控机制，又对各组的心肌细胞进行了钙瞬变的测定。

All experiment animals lived well with no death or malignant arrhythmia following cell transplantation. Frozen sections of the heart showed a few blue-stained cells under the fluoroscope in the cell transplantation group 4 weeks later. This indicated that stem cells were successfully homing. Using hematoxylin-eosin staining, homing cells were distributed over the infarct zone, with the presence of single or mass. In the model control group, no blue-stained cells were determined.

细胞移植后两组大鼠均存活良好，未出现死亡及恶性心律失常，4周后细胞移植组心脏冰冻切片荧光镜检显示心肌内有少量胞核蓝染的细胞，提示干细胞成功归巢，结合苏木精-伊红染色，归巢细胞位于心肌梗死灶周边区域，呈单个或小团聚集；而模型对照组心肌切片未见胞核蓝染的细胞。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。