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Through all this test analyse Pathogenicity and Immunological status of ALV-J Inner Mongolia strain, the result showed the infected chickens began to have pathological change of Myelcytomatosis at 3-week: the main pathological changes of acidophile myelocyte were found in bone marrow and between cardiac muscle fibers,and there were many granules in the hyalotome.This kind of oncocyte proliferated in every tissue at different amount, but the cells that proliferated in bone marrow, cardiac muscle fiber, oarium were much more than other tissue, and they often developed neoplasma. At same condition,there were no abnormalty advent in control group, In immunological aspect: the increase of lymphokine and ANAE positive cells showed cell-mediated immunity was strengthend during antineoplastic infection; the emergence of ALV-J antibody of ELISA suggest there was humoral immunity exist at the same time.

结果显示:脾脏PCR检测结果表明1、2两实验组均有阳性病例出现,而对照组全部为阴性,说明实验鸡体内已有ALV-J内蒙株感染;接种病毒后3周龄开始出现骨髓细胞瘤病的病理变化:肝脏、脾脏有程度不同的肿大,镜下观察,骨髓内最早出现了与骨髓细胞相似、形态基本一致的大型圆形或椭圆形、胞核较大多为椭圆形、胞浆内含有大量嗜酸性圆形颗粒的髓细胞样瘤细胞增生,随后通过血流瘤细胞可转移到肝脏、心肌、卵巢或睾丸等组织并形成肿瘤性病灶,而对照组没有出现明显病变;免疫状态方面:以脾脏淋巴因子和ANAE阳性细胞增多说明细胞免疫在抗肿瘤感染过程中增强,ELISA检验实验鸡抗体出现阳性的结果表明同时也存在着体液免疫。

It was confirmed by Log-rank test that the cardiac allografts in group B and group C all survived longer than those in group A significantly (p.01). The survival time of the cardiac allografts in group C was also longer than those in groups B (p.05), which suggested that the recipients which pretreatment with SPC and CP was more efficient than that with CsA.(2)TGF-β1 is expressed as buffy granules in cardiac muscle and blood vessel dissepiment.

对数秩检验(log-rank test)证实,B组移植心脏的存活时间较A组明显延长,差异具有极显著性(P.01);C组移植心脏的存活时间较A组也明显延长,差异极其显著(P.01);将B、C两组移植心脏的存活时间进行比较时表明二者之间仍具有显著性差异(P.05),说明SPC和CP联合预处理对移植心脏的存活时间的延长效果优于应用免疫抑制剂CsA组。2)TGF-β1阳性表现为心肌细胞浆及血管壁内出现棕黄色颗粒。

Results As can be seen from the result of mapping velocity information of the standard model, the color of inside-lay is bright and the middle-lay is dim. From the encoding mode of image, it is concluded that the more it is close to endocardium, the quicker the muscle velocity is. This can prove the validity and visualization of our model.

结果通过标准模型的速度信息映射结果分析,可以看出内层模型的颜色较亮,而中层模型则比较暗淡,根据图像的颜色编码方式,可知越靠近心内膜则心肌的速度越大,证明此模型的有效性和直观性。

CAS is currently rely mainly on the diagnosis of coronary provocation test, including acetylcholine and ergonovine test pilot, domestic scholars have found in 201Tl SPECT myocardial perfusion imaging in the cardiac stress test was reverse redistribution is CAS One of the characteristics of patients, if the combination of exercise electrocardiography test negative as well as the rest of the clinical features of chest pain, diagnosis of coronary artery spasm highest sensitivity and can be made respectively 98% and 90% respectively.

目前诊断CAS主要依靠冠状动脉内激发试验,包括乙酰胆碱试验与麦角新碱试验,国内学者发现在201TlSPECT心肌灌注显像心脏负荷试验中呈反向再分布现象是CAS患者的特点之一,若结合运动心电图试验阴性以及静息性胸痛的临床特点,诊断冠冠状动脉痉挛的灵敏度以及特意度分别可以达到98%和90%。

CONCLUSIONCompare to STH solution ,HST solution are more effective for isolated rat heart preservation in 6 hours.

结论HST保存液对大鼠心脏的保存在6 h内有明显的保护作用,效果优于STH液;含4×10-1mmol/L NaHS的HST液保存效果较好,而高浓度H2S对心肌有损害作用。

Techniques of myocardium protection are now highly developed,and lung protection has become a focus of research.

肺损伤是体外循环心内直视手术的主要并发症之一,随着心肌保护技术的日益成熟,肺保护成为近年的研究热点。

Transcatheter aspiration of coronary thrombus is a simple and effective method to manage the acute myocardial infarction patients.

PT术是处理冠脉内血栓的一种简单有效的方法,初步观察可以改善AMI患者IRA的血流和心肌灌注。

Arterially perfused rabbit left ventricular preparations were used in the present study to simulate LQT2 by perfusing with d-sotalol. Transmembrane action potentials from epicardium and endocardium were recorded simultaneously, together with a trasmural ECG, in arterially perfused rabbit left ventricular preparations by use of 2 separate intracellular floating mocroelectrodes.

为探讨心室肌跨壁复极离散和心脏兴奋的恢复性质在长QT综合征室性心律失常发生过程中的作用,应用冠状小动脉灌流的兔左室肌楔形组织块标本,分模型组和对照组,采用浮置玻璃微电极法同步记录心室肌内、外膜心肌细胞动作电位和跨壁心电图。

Methods 20 healthy volunteers and 37 patients with CAD were involved in this study. Color changes resulted from CK timing and amplitude were observed at parasternal short-axis view, apical four-chamber view and two-chamber view; The percent of regional fractional area change in 30﹪ diastolic period , namely color kinesis diastolic index, were obtained using ICK software.

观察20例正常人和37例冠心病患者胸骨旁左室乳头肌短轴观、心尖四腔、两腔观各节段心肌舒张期心内膜位移引起彩阶的变化,并运用ICK定量分析软件计算出心内膜于舒张早期30﹪时间内位移的面积占整个舒张期内膜位移面积的百分比,即心内膜位移指数。

METHODS: The cardiac myocytes were isolated from mouse hearts and put in a hypotonic solution. Cellattached and insideout patch clamp methods were employed to recorll the swellingactivated Cl- channel current. RESULTS: Under isotonic conditions, no single channel activities were observed in most patches.

采用膜片钳单通道(细胞贴附式和内面向外式)方法,记录急性分离的小鼠心肌细胞暴露于低渗溶液时出现的肿胀激活氯通道电流。

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