心肌内的

Adenovirus 5第二军医大学博士学位论文英文摘要胸心外科专业(WAd5) and PDC-MLC-VEGF were transferred to the adenoviral packaging cell HEK 293cell by lipofectamine 2000 mediated gene transfer method to pack the virus; The desired Advectors were purified by density gradient ultracentrifuge and titrated in 293 cells afteridentified.

Ad.mlc-hVEGF165 体外转染心肌细胞的实验研究分离培养乳鼠心肌细胞，在同一 MOI 值下，Ad.mlc-hVEGF165 和 Ad.hVEGF165分别转染心肌细胞，以 RT-PCR 法和 Western 印迹分析法分别检测转染后心肌细胞内VEGF mRNA 的转录及 VEGF 蛋白的表达，以 ELISA 法检测转染后心肌细胞分泌的VEGF 蛋白质，MTT 等方法观察其对促内皮细胞增殖作用。

A rate-limiting step to long-term survival in heart transplant patients is coronary arteriopathy, which is marked intimal proliferation with lumenal narrowing of the small epicardial and intramyocardial coronary artery branches, leading to ischemic changes.

冠状动脉影响到心脏移植病人能否长期存活，它的特征有内膜增生伴有心外膜以及心肌内的冠状动脉分支管腔狭窄，从而导致缺血性改变。

Receiving signals from any kinds of cascades, a number of transcription factors have been implicated as direct mediators of hypertrophic gene expression including SPl, Elk-1, SRF, MEF-2, GATA, and LIM proteins.

目前虽然认为心肌肥大的发病机制与细胞外的心肌肥大因子刺激、细胞内的信号转导和核内的某些基因活化有关，但其最本质的特征是心肌细胞基因表达的异常。

After model reproducing, the rabbits were randomized to receive a directly intramyocardial injection of either pcDNA3-bFGF 100μg (n=19) or pcDNA3 100μg (n=18). Animals were sacrificed 2, 6 or 12 weeks later, immunohistochemistry analysis was performed to study expression of bFGF gene at protein level, histologic analysis was performed to evaluate the effect of angiogenesis induced by gene therapy.

模型制备成功后将动物分为治疗组(n=19)和对照组(n=18)，并于心肌内分别注射pcDNA3-bFGF100μg或pcDNA3 100μg，饲养至2、6、12周处死；免疫组化观察蛋白表达；行病理切片观察缺血坏死心肌面积的变化和缺血心肌内血管新生的情况。

In recent animal studies intramyocardial transplantation of ESCs or their differentiated cardiac-like cells regenerated injured myocardium and improved heart function in infarcted animal models.

新近的研究表明，在心肌梗死动物模型中，心肌内移植胚胎干细胞或由其分化成的心肌样细胞，能导致已损伤心肌的再生，并改善心脏功能。

PartⅢExperimental study of autologous BM-MNCs by intramyocardial transplantation into ischemic myocardium of AMI in swines Objective: To evaluate the effectiveness and safety of BM-MNCs by intramyocardial transplantation. Methods: 11 swines were divided into two groups: BM-MNCs group (n=6) and control group (n=5), total 2×10~8 cells.

实验动物分为心肌内移植MNCs组(n=6)及心肌内盐水对照组(n=5)，心肌内移植细胞数2×10~8；移植后4周观察移植细胞归巢情况、小血管密度、心功能及冠脉侧枝血管形成情况及可能发生的副作用。

CaN Aβ gene silencing can reduce myocardial hypertrophy in cultured cells, si1280 (21bp) of CaN Aβ gene is the most effective target site for siRNA. The method of intrapericardial injection of plasmid, microbubbles and erzymes can improve transfection efficiency of non-viral plasmid with satisfying targeted transfection. But the scope of transfected myocytes is still limited. CaN Aβ shRNA expressing plasmid transfection in vivo by pericardial injection results in decreased CaN Aβ protein expression of small part of myocytes, and CaN Aβ mRNA only shows decreased trend. The dosage of non-viral vector and the parameters of ultrasound energy should be optimized in further study.

结论RNAi技术抑制CaN Aβ基因表达可有效减轻Ald诱导的心肌细胞肥大程度；CaN Aβ基因中si1280(21bp)片段为实现RNAi的更有效片段；微泡+酶类心包腔内注射超声导入的方法可有效改善非病毒载体在体心肌的转染效率，同时具有一定的靶向性，但总的转染范围仍然有限；采用这一方法进一步进行&一肾一夹&心肌肥大模型大鼠在体心肌细胞的CaNAβ的RNAi干预，发现心肌肥大大鼠心外膜下局部心肌细胞CaN Aβ蛋白水平降低，CaN AβmRNA水平虽有下降趋势，但无统计学差异，提示质粒的用量及超声导入的参数有待进一步研究使其优化。

Methods:Baseline intramyocardial flows in left ventricular anterior wall in 15 normal subjects and in 14 patients with coronary artery diseasewere examined by CDCFI and pulse-wave Doppler.Then Albunex(0.08ml/kg)at bolus and after 15 min interval dipyridamole(0.56mg/kg)was injected within 4 mins respectively and intramyocardial flow in the anterior wall was observed sequentially.Results:In all normal subjects and 13 patients with CAD,color flow signals could be displayed scatteredly in the anterior wall.

应用冠脉血流显像技术检测15例正常人和14例冠脉造影证实为前降支中段和近段狭窄的冠心病患者前壁心肌内血流，显示清晰二维彩色多普勒信号后，应用脉冲多普勒探测血流频谱；分别在不同时刻经静脉注射Albunex 0.08ml/kg和潘生丁0.56mg/kg后，观察前壁心肌内彩色多普勒血流信号和频谱变化。

The remaining eighteen pigs, served as three viral administered groups, underwent coronary artery ligation and received intramyocardial viral injection. rAAV- GFP group (n=6), rAAV-CD151 group (n=6) and rAAV-antiCD151 group (n=6) animals respectively received direct intra- myocardial injection of rAAV-GFP, rAAV-CD151 and rAAV-antiCD151 1×10~(12 viron particles per pig, at 10 sites correspondingly.

采用三质粒共转染法包装CD151、antiCD151和GFP重组腺相关病毒(recombinant adeno-associated virus，rAAV)。2.1月龄小型猪22头，随机分为4组，分别为正常对照组4头(不予冠脉结扎和注射病毒，正常喂养)，rAAV-GFP组6头结扎冠状动脉左前降支，心肌内分10点注射1×10~(12viron particles的rAAV-GFP病毒，rAAV-CD151组6头结扎冠状动脉左前降支，心肌内分10点注射1×10~(12viron particles的rAAV-CD151病毒，rAAV-antiCD151组6头结扎冠状动脉左前降支，心肌内分10点注射1×10~(12vironparticles的rAAV-antiCD151病毒。

ML blood from each animal aorta and coronary sinus were harvested for two times, in which erythrocytes were lysed.

GCSF不仅能动员BMSC大量释放入外周血，同时还能增加CD34+ BMSC在心肌内的归巢。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。