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E and Sirus red in supersaturated picric acid solution;the amount of HA in serum was measured with the method of radiative immunity;the quantitative expression of fibrosis factor—TGF、PDGF was measured with the method of immunity histochemistry.Results:Myocardial collagen in model group increased obviously compared with normal group from the results of electron microscope、H.E、Sirus red in supersaturated picric acid solution.

腹腔注射柯萨奇B3病毒建立病毒性心肌炎慢性期心肌纤维化小鼠模型,随机分为模型对照组、通心络低剂量组、中剂量组、高剂量组,并设正常对照组,用药四周后处死动物通过透射电镜观察心肌细胞超微结构;HE染色、苦味酸天狼星红染色观察心肌胶原数量;放免法测定血清透明质酸的含量;免疫组化染色观察致纤维化细胞因子—转化生长因子和血小板衍生生长因子的定量表达。

Methods:Establish the model of myocardial fibrosis in chronic stage of viral myocarditis by injecting CVB3 into Balb/c rats,divide these rats into model group、Tong xin luo low dose group、Tong xin luo medium dose group、Tong xin luo high dose group and set up normal group,after four weeks of giving medicine execute the animals and observe the ultrastructure of myocardial cell with tramission electron microscope,the quantity of myocardial collagen was observed by staining with H.E and Sirus red in supersaturated picric acid solution;the amount of HA in serum was measured with the method of radiative immunity;the quantitative expression of fibrosis factor—TGF、PDGF was measured with the method of immunity histochemistry.

腹腔注射柯萨奇B3病毒建立病毒性心肌炎慢性期心肌纤维化小鼠模型,随机分为模型对照组、通心络低剂量组、中剂量组、高剂量组,并设正常对照组,用药四周后处死动物通过透射电镜观察心肌细胞超微结构;HE染色、苦味酸天狼星红染色观察心肌胶原数量;放免法测定血清透明质酸的含量;免疫组化染色观察致纤维化细胞因子—转化生长因子和血小板衍生生长因子的定量表达。

AIM: To observe the effect of endoxin antagonist,anti-digoxin antiserum,on endoxin level,ATPase activities,intramitochondrial total calcium concentration and gene expression of sodium pump isoforms in myocardium of rats with myocardial ischemia reperfusion.

目的: 观察内洋地黄素特异性拮抗剂地高辛抗血清对心肌缺血再灌注损伤大鼠心肌组织内洋地黄素水平、钠泵活性、线粒体总钙浓度以及钠泵各亚基基因表达的影响,探讨内洋地黄素在心肌缺血再灌注损伤中的作用及其机制。

The presence of reversible RWMA is able to stratify patients with severe angiographic stenosis of 75% or more from those less than 75% with a high positive predictive value.

在明显冠心病的诊断中,与ETT相比,非门控心肌显像具有更好的诊断价值,门控心肌显像比非门控心肌显像的诊断价值更好,其中尤以诊断特异性的提高更为明显。

Angiectasis, permeability were increased , inflammatory cell were transuded; The cardiac muscle injury conditions after hypoxia 4 h were similar with hypoxia 2h group, appeared lipid droplet, implied the oxidative metabolism of lipid got obstacle started from then; The cardiac muscle injuried conditions were got little improvement in hypoxia 6 and 8 hours team, mitochondria were increased and aggregatived, normal cell junction, sarcomere contract weaked; in hypoxia 10h group , mitochondria were increased and aggregatived obviously, a few glycogenosome were assembled, transverse tubule were expanded, all the changes may be the adaption of Gansu zokors in the hypoxia tolerance process to 4% hypoxia extent gradually.

在4%严重低氧耐受过程中,甘肃鼢鼠心肌超微结构在2h组出现血管扩张,通透性增加,有炎细胞渗出,异染色质边集;4h组心肌损伤状况与2h组接近,出现脂滴,提示脂类的氧化代谢在此时开始发生障碍,6和8h组心肌损伤状况逐渐好转,线粒体聚集增多,细胞连接正常,肌小节收缩较前有所减弱;10组线粒体增多聚集,线粒体脊增粗更为明显,少量糖原颗粒聚集,脂滴较前有所减少,横小管扩张增大变粗,出现了与能量代谢有关的结构适应性变化。

Transmembrane action potentials were recorded simultaneously from endocardium, epicardium, together with a transmural ECG, in arterially perfused left ventricular preparations by use of 2 separate intracellular floating microelectrodes. The stimulus-response- interval of the epicardium and the incidence of ventricular tachycardia were observed as well.

灌流全程同时采用浮置玻璃微电极法同步记录内膜下心肌、外膜下心肌跨膜动作电位和跨壁ECG,并观察心外膜下心肌的刺激反应间期(Stimulus-response-interval, SRI)和室性心动过速的诱发率。

More and more evidences have shown that traditional high 〓 cardioplegic solutions increase energy and oxygen demand during asystole; make more calcium entry into cells.

愈来愈多的研究表明:传统高〓心肌保护液于诱导心脏停搏期间增加心肌能耗、氧耗,介导〓内流并对传导系统抑制作用相对较弱,高〓所致的血管内皮细胞损伤也引起学者们的高度重视,如何减轻或避免高〓心肌保护液的不利影响,是心外科领域中一个亟待解决的难题。

Objective: To observe the effect of the herbicide butachlor on myocardial contracts dint and myocardial enzymogram of Bufo bufo gargarizans, and to study physiological toxicity of herbicide butachlor on myocardial of Bufo bufo gargarizans.

目的:观察除草剂丁草胺对蟾蜍心肌收缩力和心肌酶谱的影响,探讨除草剂丁草胺对蟾蜍心肌的生理毒性。

Objective:To observe the effect of the herbicide butachlor on myocardial contracts dint and myocardial enzymogram of Bufo bufo gargarizans,and to study physiological toxicity of herbicide butachlor on myocardial of Bufo bufo gargarizans.

目的:观察除草剂丁草胺对蟾蜍心肌收缩力和心肌酶谱的影响,探讨丁草胺对蟾蜍心肌的生理毒性。

In order to investigate the expression of Lrrcl0 during cardiogenesis, an in vitro cardiogenesis model was developed by using P19 cells which underwent differentiation induced by DMSO.

为探讨Lrrc10在心肌细胞分化过程中的表达,采用DMSO诱导P19细胞分化,建立体外心肌发生模型,显微镜观察细胞形态变化,以心α-MHC和β-MHC作为心肌分化的标志,RT-PCR检测分化过程中各基因的表达。

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