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Methods: The hEGcs from the gonadal ridges and dorsal mesenteries of human embryos aged 5-10 weeks were cultured with or without cytokine in culture medium to observe their growth and the effects of different cell culture systems were compared.

目的:优化人胚胎生殖细胞的体外培养体系,并将生长状态良好的传代的人EG细胞移植用于大鼠心肌梗死模型的治疗,探讨人胚胎生殖细胞进行异种移植对大鼠心肌梗死的治疗机制。

Methods: The hEGcs from the gonadal ridges and dorsal mesenteries of human embryos aged 5-10 weeks were cultured with or without cytokine in culture medium to observe their growth and the effects of different cell culture systems were compared.

取5~10周人胚胎生殖腺嵴和背侧肠系膜,用添加和不添加细胞因子的两种培养体系对比进行组织块原代及传代培养,选取生长情况良好的传至第4代的人EG细胞,移植到急性心肌梗死大鼠的梗死心肌周围,分别于移植后1天、1、2、4周处死大鼠,以鼠抗人细胞核抗体MAB1281作为示踪剂,通过免疫组化方法检测移植细胞的分布。

Methods: the hegcs from the gonadal ridges and dorsal mesenteries of human embryos aged 5-10 weeks were cultured with or without cytokine in culture medium to observe their growth and the effects of different cell culture systems were compared. the hegcs' from passage 4 were transplanted directly to infarcted myocardium.

取5~10周人胚胎生殖腺嵴和背侧肠系膜,用添加和不添加细胞因子的两种培养体系对比进行组织块原代及传代培养,选取生长情况良好的传至第4代的人eg细胞,移植到急性心肌梗死大鼠的梗死心肌周围,分别于移植后1天、1、2、4周处死大鼠,以鼠抗人细胞核抗体mab1281作为示踪剂[1],通过免疫组化方法检测移植细胞的分布。

Rapid fluid infusion can improve myocardial local blood flow and lessen the injury of myocardia.

快速补液能够改善心肌局部血流量,从而可减轻心肌损害。

The experimental myocardia infarction was used to observe the different reaction to sotalol between normal portion and ischmia portion of heart.

采用实验性心肌梗塞方法,以观察正常心肌与缺血心肌对索他洛尔反应的差别。

Other techniques provide indirect assessments of myocardia viability, such as myocardial function from echocardiography or myocardial fibrosis from MRI.

其他技术可间接评价心肌活性,如用超声心动图判定心功能或用MRI判定心肌纤维化。

Rapid flui d infusion can improve myocardial local blood flow and lessen the injury of myocardia.

快速补液能够改善心肌局部血流量,从而可减轻心肌损害。

QDTMT is effective for decreasing myocardial glycogen consumption in rats with myocardia IRI. It may be one of the mechanisms that QDTMT lessens the myocardial IRI.

QDTMT能减少IRI心肌细胞内糖原消耗,这可能是QDTMT减轻心肌IRI的机制之一。

Methods The model of myocardia ischemia was made by injection of pituitrin in rats. The changes of ECG were observed with three parameters, viz. the heart rate, the locomotions of T wave and S T section.

采用垂体后叶素法建立心肌缺血动物模型,以Ⅱ导联心电图T波和S—T段位移及心率的变化为指标,观察党参和黄芪对实验性心肌缺血大鼠的保护作用。

Objective To quantitatively analyze the characteristics of regional myocardia l velocities of paced heart over His bundle, and explore the myocardia l velocity pattern.

目的定量分析希氏束人工起搏状态下的心肌运动速度特征,探索心肌运动模式。

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