心收缩异常

At the one-cell stage, each embryo was injected with gradient doses of 2-8ng MO. The control morpholino, was purchased from Gene-Tools.To test the knockdown effectiveness of the morpholino, the tbx2-EGFP (enhanced green fluorescent protein) construct was generated. And coinjection of morpholino with mRNA tests its specificity of the morpholino. Overexpression of tbx2 via mRNA microinjection helps us understand gaining function of tbx2. Results The tbx2-EGFP construct tests the knockdown effectiveness of the morpholino. Zebrafish embryos lacking tbx2 function have defects in cardiac contractility, rhythm and morphology in a dose-dependent manner. The embryos with c-MO are normal in development. Coinjection of morpholino with mRNA rescue the phenotype of tbx2 knocking down. The phenotypes include pericardial edema, hypogenetic ventricle, dilation of atria, arrhythmia, bradycardia, asystole, abnormal atrioventricular canal, aberrant valve and blood regurgitation.

结果荧光蛋白融合标记实验验证了tbx2-MO可以很好得阻抑斑马鱼胚胎tbx2的表达，tbx2-MO组胚胎在心脏收缩性、节律和形态学发生上出现了异常，心脏畸形随着注射剂量的增加而出现比较一致的表型，而c-MO组胚胎没有出现明显的畸形；tbx2-mRNA和tbx2-MO的共注射减少了tbx2-MO胚胎心脏畸形的发生率及减轻了心脏畸形的程度，验证了所设计合成的tbx2-MO对tbx2基因的抑制作用具有特异性；tbx2-MO组胚胎心脏缺陷包括心室发育不良、心房扩张、房室管和瓣膜异常以及心率缓慢、心律不齐、心脏停搏、血液返流等，并根据心脏发育异常的程度分为轻、中、重度畸形组tbx2-mRNA基因过表达胚胎没有见到心腔的形成。

Results: Thrombus within right ventricle and main pulmonary artery was detected in 6 patients, right atrium and RV enlargement was found in 19 patients, right ventricular hypokinesis was found in 21 patients, hypokinesis of interventricular septum was detected in 13 patients, MPA and right pulmonary arterial dilation was found in 22 patients, marked tricuspid regurgitation was detected in 23 patients, pulmonary hypertension was found in 23 patients.

右心异常改变者26例（包括超声直接检出血栓的6例），其中右房室增大19例；右室收缩功能减退21例；室间隔左移、左室短轴切面室间隔向左室膨突呈&D&型改变1例；室间隔运动幅度减低13例；主肺动脉及右肺动脉增宽22例；三尖瓣中量以上返流23例；肺动脉收缩压增高23例，范围在33~81mmHg (62.87±23.46 mmHg)，治疗后肺动脉压明显下降，范围在21~52 mmHg (34.26±13.52 mmHg)。

Meanwhile, six segments without delayed enhancement and abnormal wall motion before PCI, were found to be hypokinetic postoperate. Four of them were depicted with low signal intensity in first-pass perfusion MR imaging before PCI.

同时发现6段无延迟强化且室壁运动正常的心肌节段在随访后出现收缩运动异常，而4段术前心肌灌注成像呈低信号区。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。