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At the one-cell stage, each embryo was injected with gradient doses of 2-8ng MO. The control morpholino, was purchased from Gene-Tools.To test the knockdown effectiveness of the morpholino, the tbx2-EGFP (enhanced green fluorescent protein) construct was generated. And coinjection of morpholino with mRNA tests its specificity of the morpholino. Overexpression of tbx2 via mRNA microinjection helps us understand gaining function of tbx2. Results The tbx2-EGFP construct tests the knockdown effectiveness of the morpholino. Zebrafish embryos lacking tbx2 function have defects in cardiac contractility, rhythm and morphology in a dose-dependent manner. The embryos with c-MO are normal in development. Coinjection of morpholino with mRNA rescue the phenotype of tbx2 knocking down. The phenotypes include pericardial edema, hypogenetic ventricle, dilation of atria, arrhythmia, bradycardia, asystole, abnormal atrioventricular canal, aberrant valve and blood regurgitation.

结果荧光蛋白融合标记实验验证了tbx2-MO可以很好得阻抑斑马鱼胚胎tbx2的表达,tbx2-MO组胚胎在心脏收缩性、节律和形态学发生上出现了异常,心脏畸形随着注射剂量的增加而出现比较一致的表型,而c-MO组胚胎没有出现明显的畸形;tbx2-mRNA和tbx2-MO的共注射减少了tbx2-MO胚胎心脏畸形的发生率及减轻了心脏畸形的程度,验证了所设计合成的tbx2-MO对tbx2基因的抑制作用具有特异性;tbx2-MO组胚胎心脏缺陷包括心室发育不良、心房扩张、房室管和瓣膜异常以及心率缓慢、心律不齐、心脏停搏、血液返流等,并根据心脏发育异常的程度分为轻、中、重度畸形组tbx2-mRNA基因过表达胚胎没有见到心腔的形成。

Myspalax cansus (Rodentia, Myospalacinae, Myspalax)which lives on the loess plateau of our country. This unique underground rats life-long living under the cave, it developed many hypoxic tolerance protect mechanism to adapt its special circumstances at in organsall levels. In this study,we used Gansu zokor and SD rats (Sprague-Dawley)for the object, do serious hypoxia tolerance experiment, to explore the hypoxia tolerance extreme of Gansu zokor, analysis the difference hypoxia tolerance between Gansu zokor and SD rats at same oxygen concentration; The changes on their cardiac muscle organization structures in hypoxia tolerance process ; The expression of HSP70 between normoxic and hypoxic control in cardiac muscle organization of two animals , to find the relativity in all the results.

甘肃鼢鼠隶属啮齿目,鼢鼠亚科,鼢鼠属,是我国黄土高原特有地下鼠,由于终生营地下洞穴生活,使得甘肃鼢鼠发展出良好的低氧耐受保护机制,本研究以甘肃鼢鼠和SD大白鼠(Sprague-Dawley)为对象,进行低氧耐受实验,探寻甘肃鼢鼠的低氧耐受极限,比较二者低氧耐受的差异;以及低氧耐受不同时程,二者心肌组织显微和超微结构的变化差异;比较分析左心室组织中HSP70的表达变化和心肌耐受过程中组织结构变化的相关性。

But the study also had several limitations. They note that although interreader reliabilities of white-matter and ventricular grades are good, estimates of sulcal grade tend to have greater interreader variability. The researchers also point out that the observed associations could be related to other differences related to fish consumption, such as a healthier lifestyle in general.

不过,此研究也有一些限制;他们指出,虽然不同判读者之间对於白质和心室等级的的信赖度不错,但脑回等级的评估在不同判读者之间有比较大的差异;研究者也指出,观察到的关联可能也与食用鱼类以外的不同状况有关,例如较健康的生活型态。

But the study also had several limitations. They note that although interreader reliabilities of white-matter and ventricular grades are good, estimates of sulcal grade tend to have greater interreader variability. The researchers also point out that the observed associations could be related to other differences related to fish consumption, such as a healthier lifestyle in general.

不过,此研究也有一些限制;他们指出,虽然不同判读者之间对于白质和心室等级的的信赖度不错,但脑回等级的评估在不同判读者之间有比较大的差异;研究者也指出,观察到的关联可能也与食用鱼类以外的不同状况有关,例如较健康的生活型态。

Conversely, in our experience,3 after 2 months of CRT, only 30%(48/162) of patients with AF presented with BVP 85% despite implementation of a rigorous rate-control protocol (rate-lowering drugs plus ventricular rate regularisation and trigger mode).

AF患者群中,如果包含这20例&临床行为&类似SR的患者,那么会使得任何结果都变得混淆。考虑到负性心率药物的低使用率和未使用调节心律的药物,报告中涉及的AF人群中的高BVP百分比是惊人的。对快速性心率失常患者恢复正常心室率这一点尤为重要。

The high BVP percentage reported in the AF group is even more astonishing considering the low use of chronotropic-negative drugs and the lack of use of a ventricular rate regularisation feature, extremely important to ensure biventricular capture in cases of fast irregular rhythm.

在Khadjooi的研究中,在包括阵发性房颤患者的AF的群体中引入了2个重要的偏倚:阵发性房颤患者两心室同步率要高于永久性房颤患者。(AF患者的BVP比例为87%);阵发性房颤患者的&临床&行为如SR患者。在AF患者群中,接受CRT的正性效果与SR人群相比要差。

Torsades de pointes is a special form of polymorphic ventricular tachycardia. It is characterized by continuously changing morphology of the QRS complexes that seem to twist around an imaginary line, rate dependence, underlying QT prolongation and the so called "long-short initiation sequence".

Torsades de pointes是一种特殊形态的多形性心室频脉,其心电图的特徵包括:QRS波的形态会一直改变,彷佛缠绕著一条假想的线;另外它的发作常与心博过缓有关,合并有QT间距延长,及以所谓「长-短」的序列而开始。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

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