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AIM: To study the influence of arachidonic acid on the action potential and L-type calcium current in rabbit cardiomyocytes.

目的:研究花生四烯酸对家兔单个心室肌细胞动作电位和L-型钙电流的影响。

Objective To study the characteristic and changes of the normal children's leftventricle systolic function in Xining area.

目的 探讨西宁地区不同年龄正常小儿左心室收缩功能的特点及其变化规律。

On this plane, the root aorta, the aorta valves, the left and right cardiac atria, the efferent tract of the left ventricle, the left atrioventricular orifice, the bicuspid valve and the right ventricle could be observed.

在该层面上可观察到主动脉根部及主动脉瓣,左、右心房,左室流出道及左房室口和二尖瓣,右心室等结构,是观察主动脉瓣和二尖瓣的理想层面。

The valve replacement with partly or whole mitral valve structure can improve the ventricular internal diameter, ejection fraction, cardiac index and stroke volume, thus improving cardiac function. However, bicuspid valve restenosis should be avoided through active observation.

保留部分或全部二尖瓣结构的瓣膜置换能更好地改善左心室内径大小、射血分数、心指数、每搏输出量,从而改善心脏功能,但要积极观察以避免出现二尖瓣再狭窄。

AIM: To explore the effects of brucine on transient outward potassium current of ventricular cardiomyocytes of rats.

目的: 探讨马钱子碱对大鼠心室肌细胞瞬时外向钾电流(transient outward potassium current,Ito)及其动力学的影响。

Results showed that L-THP possessed negative inotropic and negative chronotropic action, lowed left ventricular pressure and it's infinitesimal calculus, decreased heart output in per min., shorten 〓 and 〓 of action potential of ventricular papillary muscle of guinea pig and even the plate-phase of action potential disappeared in larger dose group , but prolonged 〓. we studied transmembrane transportation of 〓 after administering L-THP , L-THP not only depressed uptake and increased release of Ca 2+ in normal myocardium, but also it showed stronger inhibition in cell injured by ISO, and fully eliminated the effect of ISO increasing uptake and decreasing release of 〓. By detecting the 〓 content of ventricular single myocyte using fluorescent probe Fluo-3/AM, results correspond to that done above, L-THP decreased 〓 content inside of cell significantly. Using whole-cell mode, we observed the effect of L-THP on Ica in ventricular single cell, it was found that L-THP decreased Ica, the effect increased with increasing it's dose, and had voltage, frequency-dependency. Dose highly corelated to effect, corelative constant is 0.87. When washing cell with normal tyre's solution, Ica could recover, which proved the blocked action of L-THP on calcium channel once again. Meanwhile, L-THP reduced production of MDA in heart injured by ISO in dose-dependent manner, improved content of GSH-Px, in case tissue or cell free from free radical damage.

结果显示,该药物具有负性肌力和负性频率作用,降低左室压及其微分,终使每分心输出量减少;缩短豚鼠心室乳头肌动作电位时程的〓和APD〓,大剂量使动作电位平台消失,〓延长;同位素示踪法研究证实L-THP抑制〓摄取,促进〓释放,降低细胞内的〓含量;单通道水平的研究证实,L-THP减少豚鼠心室肌单细胞内向钙电流,且具有剂量,电压,频率依赖性,其作用易于洗脱,药物剂量与Ica也具有高度相关性(相关系数r为-0.87);L-THP还可抑制ISO损伤心肌组织所致的丙二醛含量增加,增加谷胱甘肽过氧化物酶的量,使组织细胞免受损伤。L-THP阻断L-型〓通道,抑制〓的摄取,促进〓的释放,降低细胞内〓含量L-THP作用的离子基础。

The restrictive filling pattern is distinguished from overfilling of a normal ventricle by a reduced and delayed E' and a shortened E deceleration time that reflect slow relaxation and increased LV stiffness.

限制性充盈模式是区别二尖瓣环流速峰值减少和延缓导致的正常心室过度充盈与更短的二尖瓣血流E峰减速时间导致的正常心室过度充盈,其反映了舒张功能变得迟缓和左室室腔僵直程度的增加。

METHODS: Open chest operation was performed on the anesthetized rats to take out their hearts. The remaining blood was washed, and the large vessels, atriums and right ventricle were removed. The left myocardium was cut into two parts, then put in the DMEM tube containing 600 mL/L of glycerol, frozen at 4℃ for 30 minutes, at -20℃ for 30 minutes, and at80℃ for 30 minutes. Then they were stored in the liquid nitrogen at196℃.

麻醉大鼠开胸后,迅速取出心脏,置于冷Krebs-Henseleit液中,洗去残存的血液,弃去大血管、心房和右心室,将左心室肌剪成两块,然后置于含600 mL/L甘油的DMEM的冻存管中,程序冷冻,即4℃30min,-20℃30 min,-80℃30 min,最后保存于-196℃的液氮中备用。

objective to observe the protective effect of qiliqiangxin capsule in rabbit heart ventricle reconstitution of chronic heart failure.methods using the method of giving rabbit abdomen aorta half ligations to build the chronic heart failure model to observe qiliqiangxin capsule the effect to blood flow mechanics index,room wall thickness,heart exponent and heart ventricle reconstitution biochemistry index.results qiliqiangxin capsule could reduce effectively bp,lvsp,lvedp,t wave,improve room wall thickness and heart exponent,reduce angii and alt,all the paramenters ahd notable difference,composed to those of model group (p<0.05,p<0.01).conclusion qiliqiangxin capsule can improve the blood flow mechanics change of chronic heart failure rabbit,which has notable inhibitory action on heart ventricle reconstitution which originate from chronic heart failure.inhibition of ang ii,alt of immune nerve endocrine system may be a mechanism of qiliqiangxin capsule.

观察血流动力学、标志心室重构的室壁厚度及心脏指数及肾素―血管紧张素―醛固酮系统相关生化指标的变化。结果芪苈强心胶囊可有效降低血压、左室压、左室末期舒张压、t波,改善室壁厚度和心脏指数,降低血清血管紧张素ii及醛固酮水平,与模型组比较均有显著性差异(p<0.05,p<0.01)。结论芪苈强心胶囊可改善慢性心力衰竭兔血流动力学的变化,对慢性心力衰竭引起的心室重构具有显著抑制作用,其机制可能与该药发挥抑制神经内分泌免疫系统rass中angii、ald的水平有关。

The higher number is measured when the heart's ventricles contract and the lower when they relax.

较大的数字是在心室收缩时测得的,较低的数字为心室舒张时测得的。

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