心包下的

Objective To evaluate the subxiphoid pericardial windowing in the treatment of large pericardial effusion and tamponade.

目的 评价剑下心包开窗治疗大量心包积液和心包填塞的疗效，总结经验。

Toxic symptom of embryo was body curvature,body atrophy,body deformity and pericardial tumefaction.

纹缟虾虎鱼胚胎在3种重金属影响下出现身体弯曲、萎缩和残缺，未能形成体节，心包囊肿等症状，还会出现较少见的胚体分叉、增生现象。

Methods Select and pitch xiphisternum bottom or cardiac apex department, paracentesis pericardii according to seldinger method and extended Central venous catheter drainage, drain Pericardial effusion, after drainage, Take the Central venous catheter for germs culture, analyse and evaluate possibility and safety of the treatment from etiology angle.

取胸骨剑突下或心尖部按Seldinger方法行心包穿刺并留置中心静脉导管，引流心包积液，引流结束通过中心静脉导管病原学结果分析评价本治疗的安全性和可行性。

Objective: Porcine enteric virus is a pathogen, which causing porcine enterovirus infection, such as porcine encephalomyelitis, reproductive failure, pneumonia, diarrhea, cardiopericarditis, myocarditis, dermal lesion, and so on . It is an important infection disease that can damage the pig-raising industries. In this study, porcine enterovirus was isolated and identificated in pocine kidney primary cell.

猪肠道病毒感染是由猪肠道病毒(Porcine enterovirus，PEV)所引起，有多种不同的临床表现，如猪脑脊髓灰质炎、生殖障碍、肺炎、下痢、心包炎和心肌炎、皮肤损伤及无症状等，是一种危害养猪业的重要传染病。

CaN Aβ gene silencing can reduce myocardial hypertrophy in cultured cells, si1280 (21bp) of CaN Aβ gene is the most effective target site for siRNA. The method of intrapericardial injection of plasmid, microbubbles and erzymes can improve transfection efficiency of non-viral plasmid with satisfying targeted transfection. But the scope of transfected myocytes is still limited. CaN Aβ shRNA expressing plasmid transfection in vivo by pericardial injection results in decreased CaN Aβ protein expression of small part of myocytes, and CaN Aβ mRNA only shows decreased trend. The dosage of non-viral vector and the parameters of ultrasound energy should be optimized in further study.

结论RNAi技术抑制CaN Aβ基因表达可有效减轻Ald诱导的心肌细胞肥大程度；CaN Aβ基因中si1280(21bp)片段为实现RNAi的更有效片段；微泡+酶类心包腔内注射超声导入的方法可有效改善非病毒载体在体心肌的转染效率，同时具有一定的靶向性，但总的转染范围仍然有限；采用这一方法进一步进行&一肾一夹&心肌肥大模型大鼠在体心肌细胞的CaNAβ的RNAi干预，发现心肌肥大大鼠心外膜下局部心肌细胞CaN Aβ蛋白水平降低，CaN AβmRNA水平虽有下降趋势，但无统计学差异，提示质粒的用量及超声导入的参数有待进一步研究使其优化。

Adult rats are divided into control group, intrapericardial injection group and negative group, sublingual vein injection group and negative group. 6ds after transfection, issues of heart, liver, lung and kidney are stained with X-gal to observe transfection to myocardium and non-target transfection. 3. CaN Aβ gene silencing in vivo. Adult rats are divided into Control group, hypertrophy model group, intrapericardial injection group and negative group, sublingual vein injection group and negative group.

二整体动物水平上探讨心肌有效转染途径：以PLacZ为报告基因，将成年大鼠分为空白对照纽(生理盐水心包腔内注射后超声导入)；转染质粒心包腔内注射组及其阴性对照组：质粒+微泡+酶的混合物心包腔内注射，超声导入；转染质粒舌下静脉注射组及其阴性对照组：质粒+微泡混合液经舌下静脉注射，超声导入。

Pericardiocentesis is a fast, safe, accurate and popular choice under the guideline of flouroscopy and contrast medium.

一旦明确诊断，积极穿刺引流是首选的治疗方法。X线透视与造影剂指示下心包穿刺引流是一种快速、安全、准确、值得推广的方法。

We successfully separated the connected liver parenchyma,sternums and the most inferior two pairs of the ribs, conjoined pleura,conjoined pericardium.

结果 术中见腹腔中有各自的消化道，共肝断面积 5cm ×6cm，胸腔中2个心包紧贴，2个胸膜紧贴，胸骨下段及 4~8 肋相连，均予完全分开。

Results They had normal separated gastrointestinal.The connected liver area were 5cm× 6cm.We successfully separated the connected liver parenchyma,sternums and the most inferior two pairs of the ribs, conjoined pleura,conjoined pericardium.

结果 术中见腹腔中有各自的消化道，共肝断面积 5cm ×6cm，胸腔中2个心包紧贴，2个胸膜紧贴，胸骨下段及 4～8 肋相连，均予完全分开。

The 4th left rib was cut and a model of left coronary artery occlusion/release was carried outo Rats were divided randomly into 4 groups: control group without LCA occlusion (group A, n=6);I/R group(group B, n=6); ketamine(5mg.kg-1)+I/R group (group C, n=6); ketamine(10mg.kg-1)+I/R group(group D,n=6)o All rats in groups B, C , D were subjected to 30 minutes of LCA occlusion followed by 120minutes of reperfusion. Rats in group C and D were injected with 5mg.kg-1 and 10mg.kg-1 of ketamine before reperfusion, respectively. Significant electrocardiogram and color changes at the area at risk were considered indicative of successful coronary occlusion and reperfusionc Serum was exampled from left jugular vein at 30 minutes and 120 minutes during reperfusion to measure IL-6 and TNF- level by ELISA measurements. After reperfusion of 120 minutes, the heart was removed and the cardiac apex was exampled with snap-frozen in liquid nitrogen and stored at -70 C .

采用左冠状动脉前降支结扎开放建立心肌缺血／再灌注模型，健康SD大鼠24只，随机分为心包打开假手术组(A组，n=6)，缺血／再灌注对照组(B组，n=6)，5 mg·kg~(-1)氯胺酮+缺血／再灌注组C组，n=6，缺血30min后右腹股静脉注入5 mg·kg~(-1氯胺酮，10mg·kg~(-1)KTM+I／R组D组，n=6，缺血30min后右腹股静脉注入10 mg·kg~(-1氯胺酮。10％水合氯醛40 mg·kg~(-1)腹腔注射麻醉，气管切开，连接多功能监护仪记录心电图；小动物呼吸机人工呼吸，呼吸频率60次／分，潮气量2ml／100g，于左胸第四肋间打开胸腔暴露心脏，在左心耳下1mm左冠状动脉处，用丝线，眼科外用不锈钢小圆针穿过心肌浅层，稳定10min后将U型含有铜丝的胶管置于冠状动脉表面一起结扎(A组不结扎，B、C、D组结扎)；结扎开始左心室心尖部即由红色变暗，30 min后呈暗红色，心电图中出现S-T段抬高，说明缺血形成。

This paper discusses design and realizable methods of remote test output interface from logical design angle.

本文从逻辑设计的角度讨论遥测输出接口的设计及实现方法。

This also 星体投射plies to buildings, structures and geological features.

这也适用于建筑物和地质特征。