德国小蠊

A recombinant protein of Bla g 2 has been obtained, which is soluble in the supernatant and therefore can avoid a process of denaturalization and renaturation of the recombinant.

获得了真核表达的德国小蠊主要变应原重组蛋白Bla g 2，该蛋白以可溶性蛋白的形式分泌到培养液中，避免了原核表达的变复性过程。

A PCR product was amplified using RT-PCR method from total RNA from the abdomens of R strain using the degerated primer. The PCR product was purificated and cloned. The plasmid is pBluescript Ⅱ KS. Recombined plasmid DNA was identified by endoenzyme. The results indicated there were different P450 genes in the PCR product.

运用这对简并引物首次从德国小蠊抗拟除虫菊酯的品系R中扩增出的特异性的DNA片段，经纯化、连接和转化，重组至pBS质粒，筛选阳性克隆，提取重组质粒的DNA，并经初步的酶切鉴定，发现存在着不同的CYP4基因。

First, the German cockroach has a larger number of eggs per capsule than the other species that infest structures.

首先，德国小蠊在生殖结构上，它的每个卵荚含有的卵比其它物种要多得多。

Objective To test toxicities of several insecticides on susceptible strains of Musca domestica and Blattella germanica in the laboratory.

目的研究室内条件下，几种杀虫剂对家蝇及德国小蠊敏感品系的毒力。

The repellent rate of clove oil and turpentine oil to Blattella germanica reduced fast by the time, especially the turpentine oil showed very weak repellent effect in a week.

在持效性上，丁香油和松节油对德国小蠊的驱避性都随时间推移有明显的递减，特别是松节油，一周后效果已很弱。

The efficacy of three formulations to B.germanica was evaluated with a small container and a quadr-cage olfactometer system. Results The linalool deploied with aethylenum-Vac granule and activated alumina granule had obvious repellency to B.germanica, and 97.7% repellency of the former was more effective than the latter.

结果 芳樟醇-乙烯醋酸乙烯酯颗粒剂与活性氧化铝颗粒剂对德国小蠊有明显的驱避性，其中芳樟醇-乙烯醋酸乙烯酯颗粒剂优于后者，驱避性高达97.7%，而芳樟醇。

Methods The optimal concentrations of CarE and substrate, pH, temperature and reaction time of the reaction system, were determined by orthogonal matrix methods.

用正交试验方法研究酶液浓度、底物浓度、反应体系的pH值、反应温度、反应时间5个因素对德国小蠊CarE活性的影响，并从试验组合中选出最优的条件组合。

The study suggested that the optimal method not only enhanced the affinity and the maximum reaction velocity of α-amylase with its substrate, but also improved the accuracy and the sensitivity on the activity of α-amylase from M. separata larvae in assays, whereas it was not the optimal method for determining the activity of α-amylase from T. molitor, C. pipiens pollens and M. domestica larvae.

结果说明，在该优化体系下，粘虫α-淀粉酶与底物的亲和力增强，最大反应速度增大，测定酶活性的准确性和灵敏度显著提高；同时该优化体系也可作为测定德国小蠊α-淀粉酶活性的优化方法，但不适合作为黄粉虫、淡色库蚊和家蝇α-淀粉酶的最优化测定方法。

The reduction rates of Solenopsis invicta Buren were above 98.0% at the dosage of 1.0% and 0.5% after 14 d treatment. The values of LT50 against Coptotermes formosanus was 3600 nun with the concentration of 1000 mg/L.

结果表明，0.5%SIOC-I-013对美洲大蠊和德国小蠊的半数致死时间LT50分别为1.61d和1.38d；1%和0.5%SIOC-I-013在药后14d对红火蚁工蚁的减退率均达到98.0%以上，浓度为1000mg/L的SIOC-I-013对台湾乳白蚁的半数致死时间LT50为3600min。

However,when Sulfury fluride, Methy1 bromide and Chong-Jun-Wei were used in two drug combination separately, their insecticidal effect could be raised obviously.

结果：硫酰氟、溴甲烷和虫菌畏单一使用，在常用浓度下对大肠杆菌灭菌率在25%以下，对淡色库蚊24h杀灭率在30.13%以下；常用浓度加倍量对淡色库蚊24h杀灭率在55.20%以下；对德国小蠊和美洲大蠊的杀灭率亦仅为39.17%和43.33%以下。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。