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This is due to the fact that AFP in the sample flows through the sample pad and binds the conjugate to form a complex. This complex then migrates down to the membrane and binds to the immobilized anti-AFP antibody A2 of the test line, resulting in the immobilization of the reddish conjugate.

如果样品中含有甲胎蛋白,膜上便会出现肉眼可见的微红色检测线,这是由于样品中的AFP与标记有单克隆抗体A1的免疫金结合形成复合物,复合物向膜上移动与固定在膜上的检测线单克隆抗体A2结合反应,结果在该处形成固定的微红色线条。

In addition with blending PVC,the composite membrane forming speed and microporous structure, pure water flux and retention experiment of egg albumin solution of PU/PVC/SiO2 membrane were improved significantly.

结果显示:SiO2的加入,可改善PU杂化膜的微孔结构并优化膜的性能,在该体系中加入PVC和PU共混,可以提高成膜速度及微孔结构,水通量和截留率得到了明显的提高。

Relying on the progress in science and technology, the company has gradually perfected and improved the process of the equipment manufactures and interruptedly developed and produced hi-tech products with its unique techniques as well as established the long-term cooperative relation with Hydranautics company and Dow Chemical Company of U.S, and Grundfos of Denmark and DP of Holland, the manufactures speciall producing multistage pumps.

中空纤维超滤膜处理装置、进口膜反渗透处理装置;阴、阳离子交换器;机械过滤器,活性炭过滤器,除铁锰过滤器以及各种不锈钢微吼过滤器和各种型号的微吼过滤滤芯等,并且与国外著名企业膜元件生产制造商美国海德能公司、陶氏化学公司、日本东丽公司、韩国世韩公司和进口多级泵专业制造公司丹麦、荷兰公司、美国 ITT 公司及著名臭氧机生产厂家美国太平洋公司等建立了长期合作关系,技术应用处于国内领先。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果:1、哇巴因作用于晶状体钠泵后,光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙,电镜下全层细胞空泡化、线粒体肿胀出现髓样结构,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后,光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离,电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后,光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整,电镜下细胞器丰富、细胞功能旺盛,RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后,光镜下晶状体质膜完整、细胞排列紧密规整,电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝,RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后,光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化,电镜下囊侧膜内有高电子密度带、细胞核仁丰富,RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后,光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏,电镜下质膜变性、线粒体肿胀,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

The result of immobilization showed that hydrophile membranes were more advantageous than hydrophobe ones to act as immobilization carrier. Crosslinking organophosphorus hydrolase by glutaraldehyde on polyethersulfone membrane is a simple and practical immobilization method. An appropriate amount of bovine serum albumin and crosslinker is necessary to get a good result of immobilization. In the biodegradation of methyl-parathion through enzyme membrane reactor, the effect of immobilized enzyme amount, flow rate of peristaltic pump, pH of the feed, and the methyl-parathion concentration on biodegradation rate was studied. It was shown that the biodegradation rate increased with immobilized enzyme amount and substrate concentration. Biodegradation rate didn't increase when the folw rate of the peristaltic pump was larger than 7 ml/min.

然后,研究固定化酶降解甲基对硫磷,从不同材料的膜载体固定有机磷水解酶的比较中,发现亲水膜比疏水膜适合作固定化酶载体;在此基础上,将有机磷水解酶固定在聚醚砜微滤膜上,并制成酶膜生物反应器,用于降解甲基对硫磷;戊二醛化学交联法固定时,酶液与10%牛血清白蛋白溶液的比例在3:1~2:1,与交联剂的比例在7:2~7:4范围内,固定化效果较好,并发现牛血清白蛋白和膜载体有降低酶活损失的作用;将固定化的酶膜装于酶膜反应器降解甲基对硫磷,实验结果表明:反应器的降解速率与固定化酶量呈正比,也随底物浓度的增加而加快,当流速低时,降解速率随流速增大而加快,到7ml/min以上时,降解速率不随流速脑黾佣洌到獾淖畹团ǘ任?

Both overlong or overshort bottom duration and overlong or overshort fluctuation period are unfavorable for reduction of non-diamond content. Only moderate ones have optimal effects on it.3 Compared with a smooth microwave, a fluctuating one can reduce the diamond nucleation density slightly.4 In fluctuating microwave, even if a higher CH〓 concentration chosen, diamond films with quality better than that of those deposited at a lower CH〓 concentration and in smooth microwave can be obtained.In smooth microwave, the influence of O〓 upon the diamond films'MWPCVD was investigated. It is found that:1 In the range of lower O〓 concentration, its increase is beneficial to improvement of quality of diamond films, but in that of higher one, its increase conversely deteriorates the quality of diamond films.

在脉动微波下进行了沉积金刚石膜的研究,得到下述结果:1)采用合适的脉动微波,能使金刚石膜的沉积质量显著提高,并能使金刚石膜中的微晶石墨得到几乎完全的抑制。2)过长或过短的平底时间或脉动周期均不利于金刚石膜中非金刚石相碳含量的降低,只有适中的平底时间或脉动周期才有最佳的降低非金刚石相碳含量的效果。3)脉动微波会使金刚石的形核密度有所下降。4)脉动微波下,即使选用较高的CH〓浓度,也能得到沉积质量比在平稳微波下采用较低的CH〓浓度所制备的金刚石膜的沉积质量要明显提高的金刚石膜。

Experimental results revealed that triethanolamine has remarkable effect on arc restraining,which ...

实验结果表明:三乙醇胺可以有效抑制火花放电,增加膜层的厚度,使表面孔隙变小,提高表面光洁度;当三乙醇胺浓度为30g.dm-3时,膜层耐蚀性能最好;在阳极氧化过程中,三乙醇胺化学吸附于镁合金表面,从而改变微弧氧化过程中氧气气泡在镁合金表面的吸附强度和氧气气泡的大小,降低了微弧氧化陶瓷层孔隙率,提高了阳极氧化膜的致密性和耐蚀性。

However, if the amplitude excessed 90%, the mounts of larger bubble increase as the increasing time.

另外,选择惰性气体SF6作为微泡内气体,设计了基于两种非离子表面活性剂(Span 60和Tween 80)的包膜超声造影微泡的膜材料组成,使用声振法制备得到表面活性剂包膜的超声造影微泡;对制备过程中的声振条件进行优化,结果表明总体上振幅越大,处理时间越长,声振后溶液的总体积增加得越多,即产生的微泡量越大,并且平均粒径越小,但当声振幅度增大到90%时,随着声振时间的延长,主要增加的是大微泡的量,有效微泡的量无明显增加,因此最佳的声振条件选择为振幅90%,声振时间60s。

Results of the experimental groups: Comparison with the control groups, in 6h and 8h groups eminences of ependymal cells with cobblestone-like appearance were seen, their microvilli were further richer, and a number of microholes could be found on their base. In body part of SFO cell membrane of some ependymal cells were broken completely, their cytoplasm was no longer seen, and cell nucleus located at one side of the cell, 5-7μm in diameter and dividing lobe-like in shape. Beside those cells some ependymal cells appeared many folds on the top of surface. In 2h and 16h groups the observation results seemed no significant differences from the corresponding control groups.

实验组结果:与对照组相比,在6小时和8小时实验组中,可见成团的鹅卵石状室管细胞隆起于表面,微绒毛更为丰富,微绒毛根部可见到若干微细小孔,在SFO体部的一些室管膜细胞的胞膜完全破裂,胞质几乎荡然无存,仅剩胞核居于细胞的一侧,直径有5.7μm,呈分裂叶状;与这些细胞邻近的室管膜细胞表面最高处多呈现皱襞。2小时与16小时的实验组的观察结果与对照组相比未发现明显差异。

Through differential centrifugation and sucrose density gradient centrifugation,tono plast vesicles were isolated from suspension-cultured Populus euphratica cells broken by mortar and pestIe,blender or ultrasonic homogenizer.The sensitivities of H+-ATPase to NO-3,VO3-4 andNaN3,as well proton pnmping activities of membrane-bound pyrophosphatases demonstrated that tonoplast vesicles mainly distributed on the interface of 0%~25%of sucrose.

为进一步研究液泡膜及H+-ATP酶在胡杨抵御盐胁迫中所起的作用,比较了研磨、捣碎和超声破碎三种细胞破碎方法,从悬浮培养的胡杨细胞中制备液泡膜微囊的效果;并用差速离心和不连续蔗糖密度梯度离心纯化了胡杨液泡膜微囊。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

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