微胞的

This project studies the scale-dependent deformation behavior of the metal matrix composite, the void nucleation and void growth mechanisms at the micron/submicron scale. The main results are:(1) In prophase, growth and coalescence of the void embedded in the graded matrix are analyzed in detailed;(2) Based on the infinite solid model containing a micro-void, coupling effects of the void shape and the void size on the void growth are studied carefully, the results show that it seems to exist a critical equivalent void radius, which is associated with the material length. When radius of a microvoid is close to or smaller than the critical void radius, the micro-void growth rate is essentially eliminated;(3) The coupling effects of the particle shape and size on the mesoscopic stress fields within the particle and matrix are also investigated by introducing the conception of inclusion/matrix interfacial energy. The results show that the stress concentration factors within the particle and on the matrix/particle interface are also strongly size-dependent,so the void nucleation mechanism is size-dependent.(4) By employing a specific orthogonal curve coordinate frame and a 'kernel function' conception, a 'unified method'solving the spheriodal and spherical void problems is suggested; by this unified method, size-dependent plastic potentials of the porous materials containing the spheriodal or spherical voids are obtained, which extend the traditional Gurson model for the spherical void and GLD model for the spheriodal voids to the micron scale.(5) Based on the RVE model containing the spheriodal or spherical particles, the influences of the particle shape and size on the size-dependent mechanical behavior of metal matrix composite are studied.

中文摘要：本项目对金属基复合材料在微细观尺度下的尺度相关变形行为、孔洞形核及长大的机理和模型进行了研究，取得了如下主要结果：1）在前期研究中，探讨了基体的梯度分布对孔洞长大和聚合的影响；2）基于含孔洞的无限大体模型，探讨了孔洞形状和孔洞尺寸对其长大的耦合作用，结果表明：可能存在一个与材料特征长度相关的临界孔洞尺寸，当椭球孔洞的等效半径小于临界孔洞半径时，孔洞的长大受到明显抑制；3）通过引入基体/夹杂界面能的概念，分析了夹杂尺寸、夹杂形状对材料细观应力场的耦合影响，结果表明：颗粒内部和界面上的应力集中因子强烈地依赖于夹杂的尺寸和形状，因此，孔洞的形核机理是尺寸相关的；4）采用一种特殊的正交曲线坐标系和引入"核函数"的概念，"统一"地得到了含椭球和球形孔洞的材料的尺寸相关塑性势，它将传统的Gurson球形孔洞模型和GLD椭球孔洞模型推广到微尺度范围；5）基于含椭球和球形夹杂的体胞模型，初步研究了夹杂形状、夹杂尺寸对金属基复合材料尺寸相关力学行为的影响。

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果：1合成的CS-PAA磁性聚合物微球呈球形，大小均一，TEM测定其干态下粒径为80nm左右，磁化曲线证实具有超顺磁性，具有一定的PH敏感性，固载表柔比星后，水溶液性状稳定，无沉淀物，DLS测定直径约200nm左右，测定载药率为15％，较文献报道高，包封率为20％。2肉眼观察试验组大鼠膀胱表面呈褐色，可见大量的Fe粒子聚集，普鲁士兰染色法显示，试验组大鼠膀胱壁内有大量的Fe粒子，分布至膀胱壁全层，与对照组大鼠相比，试验组大鼠的肝、脾内的Fe粒子聚集量明显降低；HPLC测定结果与Fe染色相同；高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高，其他组大鼠血生化指标没有明显变化。3MTT法发现，高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组，FCM发现试验药物组可引起明显的肿瘤细胞凋亡，试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C，试验组细胞Bcl-2蛋白减少，Bax蛋白变化不明显，Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P＜0.01)，其中高剂量组的抑制率最高。

The location and structures of sex-pheromone-producing gland in female H.insularis were studied by EAG,GC,SEM,and TEM.These studies showed that thegland situate in the intersegmental membrane between the eighth and ninthabdominal segments,and is an eversible abdominal fold;Many plump cones disturbon the surface of the gland.The glandular cells of 2-day old virgin female H.insularis are arranged in one layer,among which the central cells are columnarepithelial cells and flat on two sides.The nucleus is irregular elliptical.There isevident conjugation between cells and the involution is more in the basal membraneof cell.Microvilli are distributed on the cytoplasmic membrane and linked withendocuticle on which there are many layers of chitin,and the outer cuticule is staineddeeper.The cell contains bubbles,mitochondria,glycogen deposits,roughendoplasmic reticulum and smooth endoplasmic reticulum.

结合触角电位、毛细管气相色谱、扫描电镜、透视电镜等技术对小线角木蠹蛾雌蛾腹尖末端不同组织部位提取物的测定分析以及腺体位置和形态结构的观察发现：小线角木蠹蛾性信息素分泌腺位于腹部末端8～9节之间，是一个由节间膜特化而成的上皮结构，为一可外翻的腹褶，腺体表面分布着饱满的锥形体，羽化后2天未交尾的雌蛾腺体细胞呈单层排列，腹面中央由密集的柱形上皮细胞组成，细胞排列向两侧延伸至背部，其形状由柱形逐渐变为扁平形，细胞核为椭圆形，细胞与细胞间有明显的胞连接，细胞基底膜基褶较多，质膜上分布着微绒毛，并与内表皮连接，内表皮上有多层几丁质，外角质层染色较深，细胞质中含有空泡、线粒体、脂质粒、粗面内质网和光面内质网。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。