微胞的

In the unsterilized soil, the colonization of JMC1402P was affected by the physical and chemical factors of soil, and the aboriginal microbe would compete with it.The safety of releasing JMC1402P to the surrounding was tested, the design was as follows: mixing JMC1402P and R. huakuii JS5A16L, JMC1402P and Pseudomonas fluorescens Pf.X16L2 in filterable membrane, sterilized soil and unsterilized soil.

JMC1402P标记菌株释放环境的安全性研究表明，当JMC1402P与华癸中生根瘤菌JS5A16L、荧光假单胞菌Pf.X16L_2混合培养在微孔滤膜上、灭菌盆装土及田间三个环境中时，未检测到华癸中生根瘤菌JS5A16L和荧光假单胞菌Pf.X16L_2中有gfp基因编码的绿色荧光蛋白的存在，在X-GlucA作用下亦没有带有gusA基因的发光菌落。

This paper reviewed the advances in honeybee breeding involving to estimate the numbers of subfamilies in a colony and analyze the genetic correlation among workers; guess the polyandry level of queens and identify the purity of a subspecies; build a linkage map and discriminate loci of quantitative traits; genotype and distinguish full sister from semi sisters; analyze the trend of sperm to inseminate an egg and so on by means of such molecular biotech as microsatellites DNA, mitochondria...

综述了应用微卫星 DNA技术、线粒体 DNA技术、随机扩增多态性 DNA、聚合酶链式反应和 DNA指纹等分子生物技术所进行的蜜蜂育种研究现状。其应用主要包括估计蜂群内亚家系的组成数目和分析工蜂间的遗传相关，推断蜂王的多雄水平和鉴定蜜蜂种性，构建连锁图和区别数量性状位点，鉴定基因型和区别全胞、半胞姐妹以及分析精子受精力的倾向性等

The influence of AHLs and its analog on the biosynthesis of MAAs and scytonemin were studied in this paper.

研究了AHLs及结构类似物对水华蓝藻铜绿微囊藻FABHE905和集胞藻（SynechocystisspFACHB898MAAS和Scytonemins合成的影响。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

Methods Lymphocytes with different MTHFR C677T genotypes from the donors were cultured for 9 days in media with different concentrations of folic acid and the frequencies of micronucleated binucleated cell was evaluated by cytokinesisblock micronucleus cytome assay.

采用9天淋巴细胞长期培养辅以胞质阻断微核细胞组分析，分析不同浓度的叶酸对三种MTHFR C677T基因型乳腺癌人群和健康人群双核细胞微核率的影响与差异。

We have found that microfilament of cytoplasm was decreased or disappeared and rate of lateral diffusion of ConA r ceptor was increased (D = 1.05 ×10-10cm2/ sec)after the cells were treated with CB. The microfilaments and the rate of lateral diffusion of ConA receptor on cell membrane were recovered D = 0.81 ?

经CB处理细胞后，胞质微丝减少或消失，但是膜ConA受体复合物的侧向扩散运动增加〔D〕=1.05×10~(-1)cm~2/sec，当除去CB后，微丝重新出现，膜ConA受体复合物的侧向扩散运动又减慢〔D〕=0.81×10~(-1)cm~2/sec，与对照组相似。

After SBF solution cultivation for some days, the Ca/P coating was very easily deposited on the porous surface that was constituted of cystiform polygon made up with irregular nanometer circles like honeycomb. The energy spectrum showed that n/n atom ratio is about 1.61:1 which is very similar to that of HA and human bone. The active surface of the porous titanium after acid-alkali treatment and SBF solution condition which calcium-phosphorus ions was supersaturated induced the formation of Ca/P deposition layer.

SBF溶液培养后发现沉积层由蜂巢状多边形胞状物组成，这些胞状物是由网状纳米环无规则排列构成，能谱测定钙、磷元素原子比n／n约为1.61∶1，与HA和人骨中的钙磷原子比很接近；通过对沉积机理的分析，认为微孔钛的活性表面和SBF溶液过饱和离子浓度的环境造成了液相中钙磷盐的沉积。

For further understanding the effect of the strain on K-bearing silicate mineral, an experiment with KO2 strain cultured in nitrogen-containing and nitrogen-free medium was conducted. By putting mineral powders into the medium and measuring the concentration of capsular polysaccharides, we concluded that the strain can accelerate the weathering rate of silicate minerals. The results showed that the polysaccharides secreted by the strain in the growth process could help the bacterial to adhere to the mineral surface effectively, and created the bacterium-mineral complex, which formed a mircro-enviorment avail the release of potassium. Another result was that there was higher level of Carbonic Anhydrase activity, which revealed that some exo-protein or enzyme produced by the bacterial had postive impact on the process of releasing potassium ion. We carried out the bacterial fermentation for larger scale production of the bacterial secretion, which was used to sperate, and identify the small molecules related to the mineral-bacterial interaction. After the analysis, it was found that the strain can produce many kinds of small molecules, such as 2-Hydroxybenzoic acid, 4-Hydroxyphenylacetic acid, methyl-4-hydroxybenzoate, etc.

分别将菌株接入含有不同矿粉的培养基中培养，检测其在有氮、无氮培养基中荚膜多糖含量的变化，并在以钾长石和黑云母为矿源的情况下，比较研究了菌株在有氮、无氮培养基中对矿物的风化能力，通过一系列的实验，证实胶质芽孢杆菌在生长过程中所分泌的粘稠胞外聚糖可帮助细菌有效黏附在矿物表面，形成细菌-矿物复合体，并改变及维持该复合体内部的微环境，有助于该菌的解钾作用，而且细菌分泌的胞外蛋白质在该菌对含钾硅酸盐矿物的解钾作用过程中发挥重要作用；将胶质芽孢杆菌接入以钾长石和黑云母为矿源的有氮、无氮培养基中培养，检测到该菌碳酸酐酶活性的变化，并进行批量发酵后小分子酸性分泌产物的提取、分离、纯化与鉴定等方面的研究，结果表明，该菌可产生2-羟基苯甲酸，4-羟基苯乙酸，4-羟基苯甲酸甲酯等小分子物质。

The basolateral M cell surface is invaginated to form an intracellular central pocket into and out of which lymphocytes and macrophages.

M细胞是一种特化的上皮细胞，多分布于Peyer's结圆顶区的周边，FAE的顶端未见M细胞；其结构有别于相邻的肠上皮细胞，表面没有长而整齐的微绒毛，取而代之的是粗而短的微皱褶，胞质内终末网不发达，溶酶体较少，基底膜向顶部穹隆状突起，形成一&口袋&样结构，其中含有多个免疫活性细胞。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果：(1)层粘连蛋白组处于G_2～M期细胞比例较对照组显著提高，Go～G_1期细胞比例显著下降，提示层粘连蛋白促进内皮细胞DNA合成，及细胞分裂增殖；光镜下，对照组细胞分布成团状，细胞密度较低，细胞间连接紧密，细胞内微丝结成网状，细胞核大小一致；与对照组相比，层粘连蛋白组细胞生长旺盛，细胞密度高，向周边伸出大量板状及丝状伪足，细胞内微丝增多、拉长、集结成束，伸入伪足中，细胞核形状大小不一致；(2)倒置显微镜观察及细胞生长曲线显示，各组细胞数目随时间增加而明显增多，各实验组较对照组增生显著，EGF和LN联合应用组各时间点细胞数目最高；实验组处于S期和G_2～M期细胞数目增加，Go～G_1期细胞数目减少；提示EGF、LN单独及联合应用均可促进细胞增殖，但尚不能认为二者有交互作用；(3)倒置显微镜下，组织培养的猫角膜内皮细胞排列成密集的单层，细胞间连接紧密；组织学观察发现，培养的猫角膜内皮细胞形成完整的内皮层，贴附于脱水基质的后弹力膜上，与正常的角膜内皮组织结构相似；扫描电镜下，组织培养的猫角膜内皮细胞间紧密镶嵌排列，可见某些细胞呈近似六边形排列，细胞大小不甚一致，胞核清晰。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。