微胞

The ciliature microtubular organelles in the ventral cortex of Urostyla grandis were visualized by direct fluorescent labeling of FLUTAX .The organelles consist of adoral zone of membranelles, undulating membranes,frontal-midventral-transverse cirri,left and right marginal cirriand the base-associated microtubules,etc.

应用荧光紫杉醇直接荧光标记显示，腹毛目纤毛虫大尾柱虫 Urostyla grandis 腹皮层纤毛器微管胞器由口围带、波动膜、额腹横棘毛和左、右缘棘毛等纤毛器微管、纤毛器基部附属微管等组成。

Notice that they are simple extensions of the apical cytoplasm, with unit membrane continuing over their surface.

观察他们都只是细胞顶端胞质和胞膜向表面突起而形成的微绒毛结构，薄膜都是连续的。

Some new organizational patterns of microtubules (that have not been described before) were observed during microsporogenesis, including the existence of a broad band of perinuclear microtubules at the diakinesis stage of development.

主要包括：(1)在细线期，从核膜向胞质衍射的微管呈螺旋状，显示核具旋转功能；(2 )在偶线期，微管的分布呈极性分布现象；(3)在终变期，核被一组微管形成的宽带围绕着。

In the unsterilized soil, the colonization of JMC1402P was affected by the physical and chemical factors of soil, and the aboriginal microbe would compete with it.The safety of releasing JMC1402P to the surrounding was tested, the design was as follows: mixing JMC1402P and R. huakuii JS5A16L, JMC1402P and Pseudomonas fluorescens Pf.X16L2 in filterable membrane, sterilized soil and unsterilized soil.

JMC1402P标记菌株释放环境的安全性研究表明，当JMC1402P与华癸中生根瘤菌JS5A16L、荧光假单胞菌Pf.X16L_2混合培养在微孔滤膜上、灭菌盆装土及田间三个环境中时，未检测到华癸中生根瘤菌JS5A16L和荧光假单胞菌Pf.X16L_2中有gfp基因编码的绿色荧光蛋白的存在，在X-GlucA作用下亦没有带有gusA基因的发光菌落。

This paper reviewed the advances in honeybee breeding involving to estimate the numbers of subfamilies in a colony and analyze the genetic correlation among workers; guess the polyandry level of queens and identify the purity of a subspecies; build a linkage map and discriminate loci of quantitative traits; genotype and distinguish full sister from semi sisters; analyze the trend of sperm to inseminate an egg and so on by means of such molecular biotech as microsatellites DNA, mitochondria...

综述了应用微卫星 DNA技术、线粒体 DNA技术、随机扩增多态性 DNA、聚合酶链式反应和 DNA指纹等分子生物技术所进行的蜜蜂育种研究现状。其应用主要包括估计蜂群内亚家系的组成数目和分析工蜂间的遗传相关，推断蜂王的多雄水平和鉴定蜜蜂种性，构建连锁图和区别数量性状位点，鉴定基因型和区别全胞、半胞姐妹以及分析精子受精力的倾向性等

Cytomixis was observed at synizesis of prophase Ⅰ in this experiment, and MN in cytoplasm and in nucleus was noticed to distribute in these granules which migrated from one PMC into its neighboring cell.

在凝线期的花粉母细胞中观察到细胞融合现象，有胞质或核内微梁骨架与穿壁转移的胞质小球和核小球内骨架相连。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

The number of autoterophic bacteria were significantly lower than the heterotrophic bacteria. Majority of 21 strains of heterotrophic bacteria that isolated from water body were Pseudomonas. Majority of 25 strains of heterotrophic bacteria isolated from sediment were bacillus, microccus and Pseudomonas.Three genus of autotrophic bacteria were sulphuret bacteria, nitrify bacteria and nitrosobacteria that were capable of oxidizing H2S, S, FeS, oxidizing NH3 to NO2 and NO2 to NO3 respectively.

试验稚鳖池水中共分离出21株异养细菌，其中优势菌为假单胞杆菌属细菌；底泥中共分离出25株异养细菌，其中优势菌为芽胞杆菌属细菌，微球菌属细菌假单胞菌属细菌，三种自养细菌分别为具有氧化硫化氢、元素硫或硫化亚铁的硫细菌；具有氧化氨为亚硝酸的亚硝化细菌和能将亚硝酸进一步转化成硝酸的硝化细菌。

At the age of 19 weeks, we have observed ciliated-mucous cells, some cells presented with mucous granules in the supranuclear cytoplasm and a ciliating border with short growing shafts, while the other cells presented with large mucous granules as well as compound cilia.

小粘液颗粒细胞呈柱状，胞浆顶部含有少量球形的粘液颗粒，细胞表面有短小的微绒毛，细胞核大呈椭圆形，胞浆电子密度高，胞浆下部有粗面内质网和线粒体，核上部有高尔基体。

In prophase, it localized on the microtubule organizing-center. In metaphase, it co-localized with α-tublin completely on spindle. In anaphase, it co-localized with α-tublin in aster and mid-zone. In telophase, it co-localized with α-tublin in cytoplasmic bridge connecting the two daughter cells.

在分裂前期，hSMP-1蛋白位于微管组织中心区域；在分裂中期，hSMP-1蛋白与组成纺锤体的微管呈完全共定位；在分裂后期，hSMP-1蛋白与星体和纺锤体中区的微管呈共定位关系；在分裂末期，hSMP-1蛋白则与两个子细胞之间的胞质桥完全共定位。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失