微胞

In chapter 5, the concept of zero-span tension known in papermaking area is for the first time introduce to wood science area to explore in-tree variation of longitudinal tensile strength of tracheids and its main influencing factors. In chapter 6, composite micromechanics and classic laminated theory are used to make a comprehensive and detailed analysis of the main influencing factors of mechanical properties of tracheids cell wall. Some experimental results acquired in the foregoing chapters are explained successfully. Results were summarized as follows: Mechanical characteristics of wood at micron scale: 1 At micron scale, the longitudinal mechanicai behavior of wood microtome section differs greatly from that of wood with normal size.

论文的第2章首先研究了微米尺度下木材的力学特性，为后面运用木材微切片拉伸技术奠定一定的理论基础；在第3章，首次考虑到了微米尺度下木材力学性质的尺寸效应，并运用木材微切片正常间距拉伸技术研究了管胞纵向弹性模量的株内变异规律；在第4章，首次利用纳米压痕技术中最新发展起来的连续刚度测量法直接在管胞细胞壁上进行纳米级的压痕实验，测量次生壁S〓层的纵横向弹性模量和硬度，从而把细胞壁力学的研究提高到一个更高的水平；在第5章，首次把造纸领域的零距拉伸技术引入木材科学研究领域，研究了管胞纵向抗拉强度的株内变异规律及其主要影响因素；第6章则运用复合材料细观力学的基本理论和经典层板理论对影响细胞壁力学性能的主要因素进行了全面而系统的分析，并为前几章的一些实验结果提供理论上的解释。

The effect of the latter was that the resulting codeposit will contain only a few PTFE particles at lower surfactant concentration, and that it will contain a lot of bubblles at higher surfactant concentration, and that a flat composite codeposit formed with densely arranged micro-cells at suitable surfactant concentration.

后者的影响是：在表面活性剂浓度过低时所沉积的镀层中只含很少量的PTFE粒子，在其浓度过高时所沉积的镀层中会夹杂许多气泡，只有在其浓度合适时才能得到平整的、由致密排布的微胞组成的复合镀层。

Results: The normal epithelial cells were intactly lined with microvilli, and lesions on the surface of Ct infected cells were showed by SEM. The expression of actin was obvious throughout the cytoplasm of uterine epithelial cells but appeared to be most intense along the apical plasma membrane on day 4, 5 and 6 of pregnancy. On the day 7, levels of actin had increased along the apical surface. The expression of actin in the infected group was weaker than that in of the normal group.

结果：SEM示对照组子宫腔可见有微绒毛的分泌细胞；实验组腔上皮细胞表面有的可见破损，微绒毛少见；免疫组化结果显示荧光强度主要定位于腔上皮细胞胞质／胞膜，妊娠第4，5，6天沿胞质顶部分布较密集，而底部较弥散；妊娠第7天顶部强度有所增加；感染组的荧光强度均弱于相应正常组，在妊娠第5天存在显著性差异(p.05)。

Immunohistochemically, cells in myoid/myofibroblastic areas showed positive staining for α-SMA, MSA and vimentin, but negative for desmin and CD34. Electron microscopic study displayed the presence of microfilament bundles, focal dense bodies and micropinocytic vesicles, consistent with those of myofibroblasts.

免疫组织化学标记显示肌样区域细胞表达α-平滑肌肌动蛋白和肌特异性肌动抗原，不表达CD34；电镜观察证实细胞含有质膜下微丝束、局灶性致密体及微胞饮囊泡样结构，与肌纤维母细胞相一致。

Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上，活细胞单层生长，胞浆较丰富，细胞核大小一致，有核仁×400 图2 SHEE培养细胞细胞核椭圆形，核仁较大，胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状，有伪足贴壁，表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁，呈星状或多角形，有丰富伪足和胞浆突，核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞，胞核和胞浆PI染色呈红色或淡红色，蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞，染色质凝集呈块状，右上角有一固缩细胞核TdT标记×400

In this study, nonionic surfactant sorbitan trioleate (Span 85) was modified with Cibacron Blue F-3GA to become an affinity surfactant (CB-Span 85) and to form affinity-based reversed micelles while dissolved in hexane. Since the CB molecule possesses a specific conformation that mimics nicotinamide adenine dinucleotide, it can bind strongly and specifically to a wide range of nucleotide-dependent enzymes such as dehydrogenases and kinases. Moreover, high protein transfer efficiency in the reverse micelles can be easily achieved by controlling the ionic strength of the aqueous phase.

本实验使用非离子型界面活性剂Span 85与蓝色染料分子Cibacron Blue F-3GA结合，形成具有亲和性的界面活性剂 CB-Span 85，由於CB染料分子具有与NAD+相似的结构，可与含核苷酸部位的蛋白质具有亲和力，将CB-Span 85溶於有机溶剂形成反微胞相，可有效的提高反微胞的选择率，可以只控制离子强度来调节蛋白质进出反微胞的效率。

Therefore, we used physical mechanism trigger drug release from liposome. In this study, the small unilamellar vesicles liposome were encapsulated hydrophilic marker drug 5(6)-Carboxylfluorescein and prepared by the thin layer hydration and extrusion methods. Liposomal suspension were exposed to the magnetic field.

因此希望以物理方式驱动微脂粒药物释放，本研究以薄膜水合法与挤压法制造单层微胞微脂粒包覆水溶性药物5(6)-Carboxylfluorescein的萤光指示剂经由磁场照射，以粒径仪、界面电位仪与萤光仪发现微脂粒经由磁场照射后使得微脂粒形体改变，粒径和带电性明显变化、萤光药物释放量增加。

The ciliates were always ideal materials in the studies of the structure and function of cytoskeleton and its cell control and adjustment, for they have many kinds of cytoskeletons with complexity and they could be easily cultured and handled.

由于原生动物纤毛虫具有多种复杂的细胞骨架结构，加之其材料培养和处理的优点，一直被作为研究细胞骨架结构与功能及其细胞调控的理想材料。20世纪80年代初，许多学者应用蛋白银染色方法和超微结构方法，显示了纤毛虫细胞皮层中以纤毛基体作为主成分的骨架结构，探讨了纤毛虫皮层纤毛器的形态、形态发生及其结构形成机理，在国内外原生动物细胞学领域成为主流。20世纪90年代起，先后应用非离子去垢剂处理和扫描电镜相结合的方法、稳定保存微管结构的透射电镜术，微管蛋白的抗体标记及其免疫荧光技术及相关的生化与分子生物学等方法显示纤毛虫的微管胞器及其微管蛋白组分，探索微管胞器的形态、功能及其微管装配机理，积累了较多的资料，取得了重要的进展。

Results of the experimental groups: Comparison with the control groups, in 6h and 8h groups eminences of ependymal cells with cobblestone-like appearance were seen, their microvilli were further richer, and a number of microholes could be found on their base. In body part of SFO cell membrane of some ependymal cells were broken completely, their cytoplasm was no longer seen, and cell nucleus located at one side of the cell, 5-7μm in diameter and dividing lobe-like in shape. Beside those cells some ependymal cells appeared many folds on the top of surface. In 2h and 16h groups the observation results seemed no significant differences from the corresponding control groups.

实验组结果：与对照组相比，在6小时和8小时实验组中，可见成团的鹅卵石状室管细胞隆起于表面，微绒毛更为丰富，微绒毛根部可见到若干微细小孔，在SFO体部的一些室管膜细胞的胞膜完全破裂，胞质几乎荡然无存，仅剩胞核居于细胞的一侧，直径有5.7μm，呈分裂叶状；与这些细胞邻近的室管膜细胞表面最高处多呈现皱襞。2小时与16小时的实验组的观察结果与对照组相比未发现明显差异。

Where the catalysts were prepared by micelle method, and followed by H-plasma pretreatment to obtain the well-distributed nanoparticles.

而其中的触媒是利用微胞合作再经过氢电浆前处理所形成分布均匀的金属奈米粒子。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失