微粒体

Then the enzyme activities of CYP2E1 in the liver microsome were determined by spectrophotometry.

结果：实验组小鼠肝微粒体CYP2E1酶活性增加，于第3天达高峰，诱导率在4.96%~84.27%之间；各组CYP2E1与-βActin RT-PCR扩增条带像素灰度的比值差异无显著性。

ArK was closely associated with the microsome fraction of the liver and plasma high densi ty lipoprotein. In 1979 Lorentz first reported that ArE decrease in hepatic disorders especially in cirrhosis and carcinoma of the liver.

在肝脏中ArE全部存在于微粒体片段，在血清中则主要与HDL结合。1979年，Lorentz首先报道该酶在肝脏疾病，特别是肝硬化和肝癌时明显降低。

Cv. Red Barbara treated with 5 mo1L^(-1) NAE(12:0) was used to investigate the effects of NAE (12:0) on the microsomal membranes composition and function in petals.

Red Barbara'进行瓶插处理，研究瓶插试验进程中NAE(12 0)对花瓣微粒体膜组分和功能的影响。

METHODS: Using phenacetin as a substrate probe, the effects of Wuji Pill with different compatibility (No.1-9) on the enzymatic activity of CYP1A2 were detected by high-performance liquid chromatography.

按L9(34)正交表，戊己丸的配比设计为9个受试复方，以非那西丁为探针药， HPLC检测戊己丸对大鼠体外肝微粒体CYP1A2酶活性的影响。

Methods: The health adult human primary hepatocyte microsome was randomly divided into 12 groups for control and itraconazole with different concentrations in the range of clinical drug blood concentration, each group have 10 samples. Control groups were added culture fluid, itraconazole groups were added itraconazole with different concentration respectively, after cultured 30 minutes, substrates (phenacetin for CYP1A2, testosterone for CYP3A4) were added and cultured for another 40 minutes.

采用健康成人肝细胞微粒体，分为对照组和不同浓度的伊曲康唑组，共12组，每组10个样本，伊曲康唑组分别加入血药浓度范围内不同对应浓度的伊曲康唑，对照组仅加入培养液，孵育30 min后，再加入CYP450同工酶1A2和3A4的相应底物再孵育40 min。

The substrates for measuring the activity of individual CYP in vitro included phenacetin, tolbutamide, debrisoquine, and omeprazole. RESULTS: Ther...

结果：CYP1A2、2C9及3A4的含量和活性具有很大的个体间变异，另外CYP2D6的活性在各样本间也有很大差异；CYP3A4(32％)是中国人肝微粒体中含量最丰富的CYP，CYP2C9(19％)和CYP1A2(16％)的含量也很可观；除了CYP1A2的含量和活性具有一定的种族和性别差异外，未发现其它CYP具有种族和性别差异；CYP1A2、2C9和3A4的酶蛋白含量分别和它们的活性具有很好的相关性。

Intestinal microsomes of sea bream fed different lipid sources (fish, soyabean and rapeseed oils) at three different inclusion levels were isolated and incubated with l-[14C]glycerol-3-phosphate and [1-14C]palmitoyl CoA.

海鱼的肠微粒体用不同来源的油脂食物营养、用不同的含量，同时单独而且用碳14标记的3-磷酸甘油和棕榈酰辅酶A。

CPZ and TFP inhibited ethylene-induced increase in activity of Ca〓-ATPase located in microsomal membrane in tomato and strawberry fruit, which indicated that 1 CaM involved in inducing of ethylene on Ca〓-ATPase; 2 the enzyme was regulated by CaM.

钙凋素拮抗剂CPZ和TFP能抑制乙烯诱导番茄果实和草莓果实微粒体膜Ca〓-ATPase活性，这表明CaM参与乙烯对该酶的诱导以及该酶活性受CaM的调节。

Methods Seven probe drugs were incubated with or without methyl protodioscin respectively in human microsomes. The specific substrates used in this study were caffeine (CYP1A2), dextromethorphan (CYP2D6), tolbutamide (CYP2C9),-mephenytoin (CYP2C19), chlorzoxazone (CYP2E1), coumarin (CYP2A6) and midazolam (CYP3A4). The concentrations of the substrate metabolites (paraxanthine, dextrorphan, 4-hydroxytolbutamide, 4-hydroxymephenytoin, 6-hydroxychlor-zoxazone, 7-hydroxycoumarin and 1-hydroxymidazolam) were determined by HPLC and LC-MS/MS. Results MPD 1-10 μmolL^(-1 had no significant inhibition on the activities of the seven cytochrome P450 enzymes.

将MPD和CYP450酶7种亚型的特异性探针底物咖啡因(CYP1A2)、右美沙芬(CYP2D6)、甲苯磺丁脲(CYP2C9)、s-美芬妥因(CYP2C19)、氯唑沙宗(CYP2E1)、香豆素(CYP2A6)及咪达唑仑(CYP3A4)与人肝微粒体进行孵化反应，采用HPLC和LC-MS/MS法测定对应的7种代谢产物（1，7-二甲基黄嘌呤、去甲右美沙芬、4-羟基甲苯磺丁脲、4-羟基美芬妥因、6-羟基氯唑沙宗、7-羟基香豆素和1-羟基咪达唑仑）的浓度，通过与对照组比较，确定MPD对以上7种酶活性的影响。

To identify the location of Pfdyn, Triton X-100 and Biotin were used to extract different cell components'proteins.

在培养三周后出现了可以抗WR99210的含稳定pHD22y-Pfdyn微粒体的恶性疟原虫的克隆。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。