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Objective To explore the mitochondria and microsome of testis cells caused by nickel sulfate in rate.

目的从氧化应激角度探讨硫酸镍(NiSO4)对大鼠睾丸细胞线粒体及微粒体的毒性作用。

Abstract摘要: Objective To explore the mitochondria and microsome of testis cells caused by nickel sulfate in rate.

摘要摘要:目的从氧化应激角度探索硫酸镍(NiSO4)对大鼠睾丸细胞线粒体及微粒体的毒性功能。

The microsome was extracted from human thyroid tissue by means of gradient centri-fugation in presence of sucrose.

本试验以梯度离心的方法,从存有蔗糖液的人甲状腺组织匀浆中提取微粒体,经洗涤后去除甲状腺细胞中的杂蛋白,特别是甲状腺球蛋白成份。

The rat's testis damage is related to the enhancement of oxidative stress of mitochondria and microsome caused by nickel sulfate.

镍对大鼠睾丸细胞的损伤可能和其所致的睾丸细胞线粒体及微粒体氧化应激效应增强有关。

Aim To study the metabolic kinetics of MN9202 in Beagle dog liver microsome.

目的 研究MN9202在Beagle犬肝微粒体酶中的代谢。

The incubation system of chicken hepatic microsome was successfully established. The activity of cytochrome P450 was determined by ultraviolet spectrophotometry.

在建立鸡肝微粒体体外孵育体系的基础上,采用紫外分光光度法测定细胞色素P450酶系的活性。

This study established a LC-MS/MS method for the determination of squamocin and bullatacin, and calculated their enzyme kinetics in rat microsome.

[目的]建立番荔枝内酯类化合物squamocin和bullatacin的液相色谱串联质谱检测方法,并测定其在大鼠肝微粒体中的酶动力学参数。

Result In rat microsome, the apparent KM of squamocin and bullatacin were (1.6±0.5)μmol/L and (2.0±0.4)μmol/L.

结果]在大鼠肝微粒体中,squamocin和bullatacin的表观米氏常数KM值分别为(1.6±0.5)和(2.0±0.4)μmol/L。

Different from the most of antioxidants, its activation factor microsome is one in five thousand of human blood cells and it can go through the blood-brain barrier, playing a role to protect the brain and the blood vessels from the radical damage increased with age.

它与其它抗氧化剂不同,它的活化因数微粒是人体血球的五千分之一,可以穿越血脑屏障,起到保护血管和大脑免受随年龄增长而增多的自由基的破坏。

Then the enzyme activities of CYP2E1 in the liver microsome were determined by spectrophotometry.

结果:实验组小鼠肝微粒体CYP2E1酶活性增加,于第3天达高峰,诱导率在4.96%~84.27%之间;各组CYP2E1与-βActin RT-PCR扩增条带像素灰度的比值差异无显著性。

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