微管

The morphology and microstructure characteristics of the apertured silver doped TiO2 microtubes were characterized by means of the X-ray powder diffraction, thermal analysis, transmission electron microscopy, scanning electron microscopy, X-ray photoelectron spectroscopy and N2 absorption.

氮吸附的结果显示该样品具有很高的比表面积，银的修饰没有显著影响二氧化钛微管的孔结构； XRD的表征结果显示，银修饰的二氧化钛多孔微管具有良好的锐钛矿晶型； XPS的分析结果表明银以游离的金属态存在； TEM的观测结果显示，银团簇沉积于二氧化钛纳米晶粒的表面。

The results indicated that the density of microtubule and neurofilament increased and the increase interrelated with axonal cross section.

由于微管参与了轴浆转运的机制，微管的增加提示其在神经再生中起了重要的作用。

The centrosome is the classical site of microtubule nucleation and is thought to be essential for axon growth and neuronal differentiation--processes that require microtubule assembly.

中心体是微管核化的经典位置，并且轴突生长和神经分化都需要微管蛋白的组装核化。

It acts by selectively binding to dimeric tubulin, inhibiting the formation of the microtubule spindle to block cell division.

它通过选择性的结合二聚体微管蛋白抑制微管纺锤体的形成阻滞细胞分裂。

Taxol have been used in clinic therapy of breast cancer. Generally speaking, taxol can accelerate the-assemble of microtubule, stabilize microtubule from its discomposing, make mitosis stagnate at G2/M, sequentially interdict the normal mitosis and proliferation of cells, induce cell apoptosis; Besides, a direct function of taxol on cell signal and gene expression, activations of apoptosis genes and expression of proteins can induce apoptosis.

紫杉醇已应用于乳腺癌的临床治疗，一般认为紫杉醇能增强微管聚合，稳定微管防止解聚，使有丝分裂停滞在G2/M期，从而阻断正常的有丝分裂和细胞增殖；除此之外，紫杉醇可以直接在细胞信号和基因表达上发挥作用，活化多种凋亡相关基因的表达，从而引发细胞凋亡。

Results Beta-tubulin genes, 1439bp and coding for 443 amino acids, was acquired and was submitted to GenBank and to get an accession number that was AY220457. The beta-tubulin gene of S. japonicum and Fasciola hepatica were homologous with an identity of 79%.

结果 获得了1个日本血吸虫新基因－微管蛋白基因，全长1439bp，编码443个氨基酸，与肝片形吸虫微管蛋白基因具有79%的同源性。

Gondii, were cloned by PCR respectively. The PCR products were digested by the corresponding enzymes and ligatd into the intermedial vectors. Finally, the inducible RNAi vector, pBSK-HSP70/5UTR-IntronC-HSP70/3UTR, with the HSP70 gene promotor as a promotor, the intron C sequence ofβ-tubulin gene as intervening sequence, 3UTR sequences of HSP70 gene as transcription stop signals, was constructed successfully, and the results of sequencing were correct. 3The construction of the inherited and inducible RNAi vector system of T. gondii: The fragment of SAG1/5UTR-eGFP-SAG1/3UTR in pBSK-SAG1/GFP vector was cloned into the vector of pBSK-HSP70/5UTR-IntronC-HSP70/3UTR to construct pBSK-GFP-Hairpin vector, then the fragment of GFP-Hairpin in pBSK-GFP-Hairpin vector was cloned into pHANA-0.5 vector.

弓形虫可诱导的反向重复序列RNAi载体的构建：设计引物，通过PCR分别扩增弓形虫HSP70基因5&UTR启动子序列(HSP70／5UTR)、HSP70基因3&UTR序列(HSP70／3UTR)及β-微管蛋白基因内含子C序列，通过酶切连接，构建以弓形虫热休克蛋白HSP70基因启动子进行驱动的，以β-微管蛋白基因内含子C序列作为间隔序列，以HSP70基因3UTR序列作为转录终止信号的反向重复序列RNAi载体pBSK-HSP70／5UTR-IntronC-HSP70／3UTR，序列测定结果正确。

Under various concentration of Ca〓, cortical MTs in young internodal cells began to slightly bend (0.5mmol/L), twist (5mmol/L), then partly disrupted (15mmol/L); but in old cells, the distribution pattern of MTs didn't change distinctly under such conditions.

而基部生长基本停止的老细胞中，即使延长Oryzalin处理时间，仍残留一些尚未完全解聚的微管小片段；三种浓度的Ca〓处理，微管的排布没有发生明显变化。

Firstly, we cloned a newβ_2-tubulin gene from the macronuclear andmicronuclear geneome of E. octocarinatus (DQ864704), respectively.

本研究首先克隆了八肋游仆虫的β_2-微管蛋白新基因(DQ864704)，与β_1-微管蛋白基因相比，基因编码区(1335bp)的同源性达到99.1％。

Induction of apoptosis by Etoposide was associated with upregulation of mRNAs for the BH3 only proteins Noxa, and to a lesser extent, Bim. In contrast, the expression levels of mRNAs for PUMA remained unchanged before and after treatment with Etoposide.

Noxa和PUMA主要受p53调节，细胞DNA损伤后通过p53可以上调Noxa和PUMA的表达［1］； Bim主要结合在微管动力蛋白上，受到细胞因子去除、Ca2+流出、紫外线照射、微管动摇和紫杉醇等刺激后可以转移到线粒体膜上［2］，从而发挥促凋亡活性。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。