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The characteristics of the acoustic response of AⅠneurons to the frequency of pure tone were investigated with extracellularly recording.

使用微电极在体胞外记录方法研究了SD大鼠AⅠ区神经元对纯音频率的反应特征。

Methods Single unit responses were recorded extracellularly with glass microelectrodes from ventral mPFC neurons in normal rats and 6-OHDA unilaterally lesiond rats in vivo .

采用在体玻璃微电极细胞外记录方法,记录正常大鼠和SNc单侧损毁大鼠的腹内侧前额叶皮质神经元的活动。

Spontaneous discharges of the rostral ventrolateral medulla neurons were extracellularly recorded by multi-barrelled microelectrodes in urethane-anesthetized rats.

实验用多管微电极细胞外记录氨基甲酸乙酯麻醉的SD大鼠延髓头端腹外侧区神经元的活动。

Unit discharges of amygdala(A2~L4.7H15.,8) were recorded extracellularly with glass microelectrode .

采用玻璃微电极通过细胞外记录杏仁核(A_(2-4)L_(4-7)H_(15-18))的神经元放电。

The effects of intracerebralventricular injection of nitric oxide donor and nitric oxide synthase inhibitor on the spontaneous discharge of magnocellular neurons of paraventricular nucleus of hypothalamus were investigated in urethane anaesthetized adult SD rats by using microelectrode extracellularly recording method.

在乌拉坦麻醉的成年SD大鼠上,用玻璃微电极细胞外记录的方法,观察了脑室内注射一氧化氮供体及一氧化氮合酶抑制剂对室旁核大细胞自发电活动的作用。

Methods:Arterial blood pressure,heart rate and the unit dischargings of habenular neurons in rat were recorded simultaneously.

记录大鼠的股动脉血压和心率的同时,用玻璃微电极记录缰核神经元自发单位放电。

The steps are as the following:1、Preparing the cerebrospinal fluid one hour before the experiment,take some of them for cryopreservation;2、Without paralyse,cuting down the rat head quickly and dislodge the hippocamp part which is used in the experment in freezing liquid,and trim the hippocamp distrct.3、Fixing the hippocamp tissue on the vibratome,to cut down a brain slice with 300μm,use the haustorial tube to move the slice to the preincubate dish,cultivanting it for one hour.Then move it to the record incubation chamfer.4、Preparing the glass micloelectrode and fill it with NaCl,the corcentation is 3mol/L.5、adjusting the recorder,after cultivate the brain slice in the incubation chamfer for 2 hours,the expermentize can be gone on.6、In order to ensure the accurate of the experimental result,use only one medicine concentration for one brain slice in every experiment.

本实验研究方法是:1、实验前1小时配制好所需脑脊液并充以95% O2和5% CO2的混合气,取小部分冷冻保存;2、在乙醚麻醉下,快速断头并在冷冻液里取出包含海马的大脑部位,修整出所需海马区;3、将海马组织固定于振动切片机上切出300μm厚的海马脑片,用广口吸管转移至预孵育皿培养一小时,后再转移到记录用孵育槽内;4、拉制好玻璃微电极,并充以3mol/L的NaCl;5、调试好记录仪器,将脑片在孵育槽内培养2小时后进行实验;6、为了保证实验结果的准确性,每一块脑薄片只进行一个浓度的药品实验。

Intrafascicular microelectrodes can stably record the electrical signals of cardiac sympathetic nerve.

神经束内微电极可稳定记录心交感神经电信号,能较好反映神经束内电活动的变化。

Intrafascicular electrodes ; Peripheral nerve ; Human ; Electric signal

神经束内微电极;周围神经;人体;电信号

In the present study, the effects of chloride channels blockers,(Niflumic acid,NFA;IAA-94;DIDS) on EJP were explored in guinea pigs, using intracellular recording techniques on acute isolated spiral modiolar artery.

本实验运用细胞内微电极记录技术,在豚鼠耳蜗螺旋动脉(spiral modiolar artery, SMA)离体标本上,研究氯通道阻断剂(Niflumic acid, NFA; IAA-94; DIDS )对去甲肾上腺素引起SMA平滑肌细胞的反应和平滑肌细胞EJP的影响。

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