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To investigate variation of soil bacteria community in response to the change in the above-ground vegetation, soil samples were collected at three sites, i.e. bamboo groves, mixture of bamboo and broadleaf tree and broadleaf forest. To explore variation of the soil bacterial structure, 16S-rDNA-V3-fragment-base DGGE (denaturing gradient gel electrophoresis) and clone sequence BLAST technologies were applied.

为了解毛竹人侵天然林后地下土壤微生物多样性的变化,分别采集了毛竹纯林、竹阔混交林和原始天然阔叶林下的土壤样品,应用建立于165 rDNA V3区片段的变性梯度凝胶电泳和克隆测序比对来研究土壤细菌结构的变化。

Results of orientation mensuration of two successive years showed that:①The mulching enhanced the difference in the number bacteria and fungi in rhizospere and bulk soil, and increased the R/B of number of edaphon in rhizospere and bulk soil.

①覆盖处理能强化玉米根区、根外细菌及真菌的数量差异,提高玉米根区与根外土壤微生物数量比值。

To obtain a better understanding of the microbiota inhabiting the intestinal tract of wood-feeding lower termites, the European subterranean termite, Reticulitermes santonensis (Isoptera: Rhinotermitidae), was used as a model system.

为了对木食性白蚁肠道共生微生物有一个全面的了解,以揭示白蚁肠道共生微生物在木质纤维素消化中的作用,本论文在国际上首次报道了应用分子克隆,DNA指纹分析,及荧光原位杂交等多种先进的分子生物学技术,以散白蚁Reticulitermes santonensis为模式系统研究低等木食性白蚁肠道内不同微生态环境中细菌的区系结构及多样化和主要细菌类群在该白蚁肠道的分布与定位等问题,并研究了与散白蚁Reticulitermes flavipes肠道共生鞭毛虫P.vertens相关的共生细菌的系统发育类型。

To obtain a better understanding of the microbiota inhabiting the intestinal tract of wood-feeding lower termites, the European subterranean termite, Reticulitermes santonensis (Isoptera: Rhinotermitidae), was used as a model system. The diversity and structure of the bacterial community in the different niches of the gut, as well as the distribution and location of major bacterial phylotypes within the intestinal tract was evaluated using various molecular techniques such as clonal analysis, DNA fingerprinting, and fluorescence in situ hybridization. In addition, the symbiotic bacteria associated with the flagellate, Pyrsonympha vertens in the gut of the closely related Eastern subterranean termite, Reticulitermes flavipes , were phylogenetically characterized.

为了对木食性白蚁肠道共生微生物有一个全面的了解,以揭示白蚁肠道共生微生物在木质纤维素消化中的作用,本论文在国际上首次报道了应用分子克隆,DNA指纹分析,及荧光原位杂交等多种先进的分子生物学技术,以散白蚁Reticulitermes santonensis为模式系统研究低等木食性白蚁肠道内不同微生态环境中细菌的区系结构及多样化和主要细菌类群在该白蚁肠道的分布与定位等问题,并研究了与散白蚁Reticulitermes flavipes肠道共生鞭毛虫P.vertens相关的共生细菌的系统发育类型。

So studying the processes and mechanisms of soil hydrology of microbiotic crusts as well as analyzing its influence on soil water conditions in fixed dunes will play an important role theoretically and practically in understanding the evolution of artificial vegetation community in fixed dunes and the security of artificial sand-fixing vegetation system.

因此,通过野外试验揭示微生物结皮对土壤水文过程的影响和机理,评估其对雨养植被固沙区土壤水环境的影响,对于认识固沙区人工植被群落的演替和人工植被固沙体系的安全性都有着重要的理论意义和实践意义。

Microbiotic crusts had a high capacity of evaporation than the shifting sand dunes. During the evaporation process of one week after rainfall, the total evaporation in artificial vegetation regions constructed in 1956, 1964, and 1981 were 6.05, 4.99 and 4.23 times respectively as the total evaporation in shifting sand dunes.

微生物结皮有着比流沙区更高的蒸发能力,在雨后一周的蒸发过程中,1956、1964和1981年人工植被区的总蒸发量分别是流沙区总蒸发量的6.05、4.99和4.23倍。

Psychrophilic and psychro-tolerant bacteria were cultured and used for screening the cold-active enzymes. Some characteristics of cold-active protease and chitinase were analyzed then. Microbial 16S rDNA clone libraries of deep sea sediments were constructed and studied by PCR-RFLP (Restriction Fragment Length Polymorphism) and phylogenetic analysis based on 16S rDNA sequences.

通过构建沉积物中微生物16S rDNA克隆文库,采用PCR-RFLP分析、DNA-DNA杂交、16S rDNA序列测定以及系统发育分析的方法,研究了西太平洋&暖池&区和东太平洋&结核&区两个不同海区深海沉积物中的微生物多样性和群落结构特征及其与环境的相互关系,得到了一些与&暖池&区环境特点紧密相关的新发现,新认识。

Psychrophilic and psychro-tolerant bacteria were cultured and used for screening the cold-active enzymes. Some characteristics of cold-active protease and chitinase were analyzed then.Microbial 16S rDNA clone libraries of deep sea sediments were constructed and studied by PCR-RFLP (Restriction Fragment Length Polymorphism) and phylogenetic analysis based on 16S rDNA sequences.

通过构建沉积物中微生物16S rDNA克隆文库,采用PCR-RFLP分析、DNA-DNA杂交、16S rDNA序列测定以及系统发育分析的方法,研究了两太平洋&暖池&区和东太平洋&结核&区两个不同海区深海沉积物中的微生物多样性和群落结构特征及其与环境的相互关系,得到了一些与&暖池&区环境特点紧密相关的新发现,新认识。

Qualitative research and quantitative analysis are given to microbial diversity in root and rhizosphere of Stylosanthes guianensis in karst area and non-karst area.

对桂林毛村岩溶区和非岩溶区柱花草Stylosanthes guianensis根系与根际微生物组成多样性进行定量定性分析。

The methods of extracting and refining tellurium presently were mainly soda roasting,autoclaving in general basic solution,sulfating roasting,oxidative leaching in acid solution, solvent extraction ,emulsion liquid membrane,microbial method,electrorefining,vacuum distillation and zone melting etc.

目前分离提纯碲的方法主要有苏打粉焙烧法、碱性高压浸出法、硫酸化焙烧法、氧化酸浸法、萃取法、液膜法、微生物法、电解精炼法、真空蒸馏法、区熔精炼法等,其中电解精炼法得到的碲的纯度达到99.99%,真空蒸馏碲的纯度达到99.999%,区熔精炼碲的纯度可达99.999995%,本文综述了以上分离提纯碲的方法。

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