微核

The smaller of two nuclei in ciliate protozoans that contains genetic material and functions in reproduction.

小核，微核原生动物纤毛虫类体内两个细胞核中较小的一个，其中含有基因材料和繁殖功能

The experiment was conducted to study the possible effects of toxicity in the toad erythrocyte s induced by dicofol,48 mature toads,Bufo gargarizans,were injected with five different dosages of DCF in coelom every 24 h and 1-4 times in succession,and cells producing micronucleus,nuclear abnormality and cell abnormality were counted separately.

用5种不同剂量的三氯杀螨醇(d icofo l，DCF)分别对48只中华蟾蜍成体进行体腔注射法染毒1~4次，观察并统计中华蟾蜍成体外周血红细胞产生微核、核异常及细胞异常的细胞数。

Microcells were produced by centrifugation of micronucleated A9 (neo12) cells in Percoll density gradient containing 20 mg/L Cytochalasin B at 39 000 g. The resulting mixture of microcells, whole cells, karyoplasts and cytoplast fragments was filtered through 8 μm and 5 μm size membrane pores sequentially to obtain pure preparation of microcells.

微核化的细胞在含有20 mg/L细胞松弛B的Percoll密度梯度介质中，经39 000g高速离心后，包含微细胞、完整细胞、细胞核和细胞碎片的混合液，依次通过8 μm和5μm孔径的滤膜过滤后可获得纯化的微细胞溶液。

Microcells were produced by centrifugation of micronucleated A9 (neol2) cells in Percoll density gradient containing 20mg/L Cytochalasin B at 39 000g. The resulting mixture of microcells, whole cells, karyoplasts and cytoplast fragments was filtered through 8 pm and 5 pm size membrane pores sequentially to obtain pure preparation of microcells.

微核化的细胞在含有20mg/L细胞松弛B的Percoll密度梯度介质中，经39000g高速离心后，包含微细胞、完整细胞、细胞核和细胞碎片的混合液，依次通过8μm和5μm孔径的滤膜过滤后可获得纯化的微细胞溶液。

Treated by higher concentration of EMS,some cells appeared micronuclei,abnormal nucleus,poly nuclei,broken nucleus,and nucleolus losing.

较高浓度EMS处理的PLB细胞中出现微核、畸形核、多核、核破裂和核仁丢失等现象。

Results Compared with solvent control,toxaphene increased significantly MN frequencies in Hep G2 cells at concentrations of 20,40?μmol/L respectively(P.01,P.01).No significant increase of MN frequencies were found in Hep G2 cells treated with Aroclor1254(23～184?μmol/L) and DDT17.8～60?

结果20，40μmol/L毒杀芬处理Hep G2细胞的微核率与溶剂对照相比显著增加(P.01，P.01)；经Aroclor1254(23~184μmol/L)和滴滴涕(17.8~60μmol/L)处理的Hep G2细胞的微核率与溶剂对照相比，差异无统计学意义。

The success was mainly due to the dilution of sea water to certain extent ( 25-50%) so that the Tradescantia inflorescences could remain alive during the time pe-riod required in the experiment.

我们发现紫露草能于海水中在实验要求的时间内成活，并通过实验证实了微核产生的原因不是海水本身，而是海水中的污染物质，污染物质含量越高，产生微核的频率也越高。

NaF can induce significant increase of micronucleus frequency in eripheral erythrocytes of Zebrafish. Neither transgenic CryIA cotton seed powder nor regular cotton seed powder has obvious effect on micronucleus frequency in eripheral erythrocytes of Zebrafish.

腹腔注射氟化钠可以诱导斑马鱼外周血红细胞微核率的显著升高；转CryIA基因抗虫棉棉籽粉粉和普通棉棉籽粉粉一样，对斑马鱼外周血红细胞微核率无明显影响。

The mutagenicity of seed powder and seed oil of transgenic CryIA cotton to mammals and fish was evaluated in this study. The raw cotton seed powder or seed oil was mixed into mice and fish's diet. Cyclophosphamide and NaF were used as positive controls respectively. Regular cotton seed powder and seed oil were used as negative controls. The micronucleus frequency in mice bone marrow polychromatic erythrocytes and in eripheral erythrocytes of Zebrafish were assayed in this study. The sperm malformation rate in mice was also investigated.

摘 要：分别以环磷酰胺和氟化钠作为阳性诱变剂，以普通棉的棉籽粉及棉籽油作为对照物，同时设阴性对照组，以成年封闭群昆明种雄性小鼠和斑马鱼为实验材料，将棉籽粉或棉籽油掺入饲料中饲喂小鼠或斑马鱼，检测小鼠骨髓嗜多染红细胞（Polychromatic erythrocytes， PCE）微核率（Micronucleus frequency， MN‰）和精子畸变率，以及斑马鱼外周血红细胞微核率。

The mutagenicity of seed powder and seed oil of transgenic CryIA cotton to mammals and fish was evaluated in this study. The raw cotton seed powder or seed oil was mixed into mice and fish's diet. Cyclophosphamide and NaF were used as positive controls respectively. Regular cotton seed powder and seed oil were used as negative controls. The micronucleus frequency in mice bone marrow polychromatic erythrocytes and in eripheral erythrocytes of Zebrafishwere assayed in this study. The sperm malformation rate in mice was also investigated.

分别以环磷酰胺和氟化钠作为阳性诱变剂，以普通棉的棉籽粉及棉籽油作为对照物，同时设阴性对照组，以成年封闭群昆明种雄性小鼠和斑马鱼为实验材料，将棉籽粉或棉籽油掺入饲料中饲喂小鼠或斑马鱼，检测小鼠骨髓嗜多染红细胞（Polychromatic erythrocytes， PCE）微核率（Micronucleus frequency，MN‰）和精子畸变率，以及斑马鱼外周血红细胞微核率。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。