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The effects of intracerebralventricular injection of nitric oxide donor and nitric oxide synthase inhibitor on the spontaneous discharge of magnocellular neurons of paraventricular nucleus of hypothalamus were investigated in urethane anaesthetized adult SD rats by using microelectrode extracellularly recording method.

在乌拉坦麻醉的成年SD大鼠上,用玻璃微电极细胞外记录的方法,观察了脑室内注射一氧化氮供体及一氧化氮合酶抑制剂对室旁核大细胞自发电活动的作用。

Because microsatellite DNA accords with Mendelian inheritance and can reflect population relationship more reliability. mtDNA is extranuclear inheritance and belongs to maternal inheritance and is not superiority in population relationship analysis.

由于微卫星DNA符合孟德尔遗传规律,能够反映群体间的亲缘关系,构建的系统发育树结构可靠。mtDNA是核外遗传物质,具有母系遗传的特点,在反映群体间的亲缘关系上不具优势。

Methods:Arterial blood pressure,heart rate and the unit dischargings of habenular neurons in rat were recorded simultaneously.

记录大鼠的股动脉血压和心率的同时,用玻璃微电极记录缰核神经元自发单位放电。

Microtubules and their textural composite hydrate system, nuclear envelope inside the cell, the places of plasma membrane infolding and cell junction and cell adhesion outside the cell possess lots of nano-scale (100-103 nm) narrow spaces.

在细胞内部的微管及其织构复合水化体系、核膜以及在细胞外部的质膜内褶和细胞连接粘连之处都可以发现许多纳米尺度(100~103 nm)缝隙空间。

It was found that, driven by the hydrogen bonding interactions, PMAA produced in-situ and HEC self assembled into PMAA/HEC nanoparticles with the insoluble interpolymer complex of PMAA and HEC as the core, and the soluble HEC as the shell.

结果表明:在大分子间氢键作用的驱动下,原位生成的PMAA和HEC自组装形成了以不溶性的PMAA/HEC大分子复合物为核、以可溶性HEC为壳的PMAA/HEC聚合物纳米微球。

Results Under TEM, the surface and nuclear membrane split, a great number of vacuoles and high electron density particles appeared in the cytoplasm in Pc trophozoites, vacuoles also did in Pc cysts, intracystic bodies became denaturation and necrosis; under SEM, filopodia and microvilli became sparse,short and fell off, a breach appeared in the surface membrane in Pc trophozoites.

结果 透射电镜发现Pc滋养体表膜和核膜破裂,胞质中出现大量空泡和高电子密度颗粒,Pc包囊中也出现空泡,囊内小体变性坏死;扫描电镜发现Pc滋养体丝状伪足和微绒毛稀疏、变短甚至脱落,表膜出现缺损。

Result Under electron microscope, the manipulus short microvilli on the SHCs' surface and high karyoplasmic ratio were observed as the presentation of dedifferentiation.

结果 电镜下观察可见SHCs表面少量短而小的微绒毛突起,核浆比例高,呈现未分化细胞的形态。

Ultrastructural observation found more karyopyknosis, abundant cellular organells and increased microvillus within cultured cells.

同时,IP6作用后细胞结构发生改变,出现核固缩、细胞器丰富、微绒毛增多等分化趋势。

Nineteen spots were changed significantly after FA treatment.Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示,甲醛刺激后19个蛋白斑点发生变化,肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点,已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ,磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依靠磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6,这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示,甲醛刺激后19个蛋白斑点发生变化,肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点,已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ,磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依赖磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6,这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

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