微核

Cerebral hemorrhage rat models were established via injection of autologous arterial blood in caudate nucleus. Two days after modeling, 5 μL BrdU-labeled human embryonic NSCs suspension was transplanted at four points surrounding hematoma cavity in the rats. After 1 and 2 weeks, rats were sacrificed. Adjacent sections were doubly stained by BrdU/microtubule-associated protein 2 (MAP-2) and BrdU/glial fibrillary acidic protein.

通过注射自体动脉血到尾状核制作大鼠脑出血模型，出血后2 d将标有5'-溴脱氧尿嘧啶的人胚神经干细胞悬液移植到血肿腔周围的4点，1，2周后处死大鼠，相邻脑组织切片行5'-溴脱氧尿嘧啶/微管相关蛋白2和5'-溴脱氧尿嘧啶/胶质纤维酸性蛋白免疫组织化学双染。

METHODS: Cerebral cortex cells of 8-week aborted human fetus were harvested and cultured in vitro to obtain human embryonic NSCs. Cerebral hemorrhage rat models were established via injection of autologous arterial blood in caudate nucleus. Two days after modeling, 5 μL BrdU-labeled human embryonic NSCs suspension was transplanted at four points surrounding hematoma cavity in the rats.

通过注射自体动脉血到尾状核制作大鼠脑出血模型，出血后2 d将标有5'-溴脱氧尿嘧啶的人胚神经干细胞悬液移植到血肿腔周围的4 点，1，2周后处死大鼠，相邻脑组织切片行5'-溴脱氧尿嘧啶／微管相关蛋白2和5'-溴脱氧尿嘧啶／胶质纤维酸性蛋白免疫组织化学双染。

Blue electronic ink microcapsules containing phthalocyanine blue BGS particles homodispersed in tetrachloroethylene were prepared by in situ polymerization using Urea-formal-dehyde as wall material.

利用脲甲醛树脂为壁材制备了以酞菁蓝 BGS颗粒分散在四氯乙烯中的悬浮液为核材料的蓝色电子墨水微胶囊。

Methods Orientated by CT,108 patients underwent pallidotomy and thalamotomy for Parkinson′s disease by means of stereotaxy microelectrode recording as intraoperation target localization.

采用 CT定位，立体定向微电极细胞电生理记录技术进行术中靶点监测，对 10 8例帕金森病人的苍白球和丘脑腹外侧核进行射频毁损术治疗。

Methods:One hundred patients underwent pallidotomy and thalamotomy for Parkinson's disease by means of microelectrode recording used as intraoperative target localization between Oct. 1997 and Apr. 1998. The patients were assessed by means of UPDRS.

采用微电极细胞电生理记录技术进行术中靶点监测，对100例帕金森病患者行苍白球腹后部(96例)和丘脑腹外侧核(4例)毁损术，患者术前及术后评估采用UPDRS积分，术中靶点更换率为87%。

It is found that the fracture of TWIP steel is typical ductile fracture pattern and its deformation and fracture mechanisms are the formation, growth and coalescence of micro-voids.

结果发现，TWIP钢具有典型的延性断裂断口特征，其变形和断裂过程为微孔洞的形核、长大和聚合。

It was found that the fracture of TWIP steel was typical ductile fracture pattern and its deformation and fracture processes were the formation, growth and coalescence of microvoids.

结果发现，TWIP钢具有典型的延性断裂断口特征，其变形和断裂过程为微孔洞的形核、长大和聚合。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

The transformation conditions and natures of acicular ferrite in the weld metal of microalloy steel s were analyzed systematically.

系统地分析了微合金钢焊缝金属中针状铁素体组织的形成条件及特点，对夹杂物粒径、数量进行了统计分析，并阐述了针状铁素体的形核位置。

The effects of the size,distribution and chemical composition of inclusions on nucleation of acicular ferrite in deposited metal s of micro-alloyed steel were studied.

研究了微合金钢熔敷金属中夹杂物大小、尺寸分布和化学成分对针状铁素体形核的影响。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。