微染

Objective To investigate the effect of gene transfection of FKBP12.6 on cardiac function and pathological changes of canine heart failure induced by rapid ventricular pacing.

目的探讨白蛋白微泡介导的pcDNA3.1-FKBP12.6质粒转染对心衰犬心功能及心肌病理变化的影响。

MATERIAL AND METHODS: The micronucleus test of mouse bone marrow cell and the sperm abnormality test in rats were used in this study.

材料与方法：采用骨髓嗜多染红细胞微核测试法和精子畸形法评价HY拮抗环磷酰胺所致的遗传物质的损伤作用。

Cerebral hemorrhage rat models were established via injection of autologous arterial blood in caudate nucleus. Two days after modeling, 5 μL BrdU-labeled human embryonic NSCs suspension was transplanted at four points surrounding hematoma cavity in the rats. After 1 and 2 weeks, rats were sacrificed. Adjacent sections were doubly stained by BrdU/microtubule-associated protein 2 (MAP-2) and BrdU/glial fibrillary acidic protein.

通过注射自体动脉血到尾状核制作大鼠脑出血模型，出血后2 d将标有5'-溴脱氧尿嘧啶的人胚神经干细胞悬液移植到血肿腔周围的4点，1，2周后处死大鼠，相邻脑组织切片行5'-溴脱氧尿嘧啶/微管相关蛋白2和5'-溴脱氧尿嘧啶/胶质纤维酸性蛋白免疫组织化学双染。

METHODS: Cerebral cortex cells of 8-week aborted human fetus were harvested and cultured in vitro to obtain human embryonic NSCs. Cerebral hemorrhage rat models were established via injection of autologous arterial blood in caudate nucleus. Two days after modeling, 5 μL BrdU-labeled human embryonic NSCs suspension was transplanted at four points surrounding hematoma cavity in the rats.

通过注射自体动脉血到尾状核制作大鼠脑出血模型，出血后2 d将标有5'-溴脱氧尿嘧啶的人胚神经干细胞悬液移植到血肿腔周围的4 点，1，2周后处死大鼠，相邻脑组织切片行5'-溴脱氧尿嘧啶／微管相关蛋白2和5'-溴脱氧尿嘧啶／胶质纤维酸性蛋白免疫组织化学双染。

In infected HF cells,microfilaments were ruptured,arranged turbulently,as well as cells merged and fluorescence density of microfilament obviously reduced.

受染细胞内微丝排列紊乱，极性消失；细胞融合，荧光强度明显减弱。

Furthermore, we encapsulated CEA transgeneic cells with micorencapsule that is made from sodium alginic acid and chitin.

进一步用以海藻酸钠和壳聚糖为成囊材料的微胶囊包裹转染CEA基因的细胞。

Labelling microcircumstance, the size of vessel opening diameter an d counterstain may also affect the result.

标记微环境，血管开口大小和复染可能影响标记结果。

Results Lactacystin dealed with C6 glioma cells in 24 h, kytoplasm was concentrated and anachomasised, the chromatin of nucleus was concentrated pyknoticly to see under photo-microscope. The microvilli on surface dispeared、nucleus crenated、chromatin aggregated pyknoticly under transmission electron microscope respectively.

结果 Lactacystin处理体外C6胶质瘤细胞24 h后，光镜下见胞浆浓缩深染，胞核染色质致密浓缩，透射电镜下见细胞表面微绒毛消失，核皱缩，染色质致密浓缩、边集。

BACKGROUND: Studies have demonstrated that ultrasound-mediated microbubble destruction significantly improves transfection efficiency of enhanced green fluorescent protein in in vitro cultured retinal ganglial cells.

背景：已有报道，超声微泡能明显提高体外培养的增强型绿色荧光蛋白基因转染视网膜神经节细胞的效率。

Heterodon specimens was successfully extracted by the steps of removing the formaldehyde through improved gradual dehydration first and extracting the genomic DNA by the traditional phenol-chloroform method then. After primers designed, the PCR amplification was carried out, and a 205 bp of the control region of mtDNA from all of the ten specimens was successfully amplified.

本实验通过改进的酒精梯度浸泡法去除标本中的甲醛，然后用酚-氯仿抽提法成功地提取到了铜鱼标本的基因组DNA；设计引物后进行了线粒体和微卫星的PCR扩增，扩增产物经银染检测。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。