微小

Based on ACIS, the WEDM oriented software for the design and mesh simulation of micro gears is developed, by which the modeling, mesh simulation and interference check can be implemented.

基于ACIS几何平台，开发了面向线切割的微小齿轮设计和啮合仿真软件，实现了微小齿轮的造型，并可进行啮合过程仿真和干涉检验。

And linking the principle of choosing computer visual inspection system hardware, the paper constructs hardware structure of visual inspection system, and develops measure software of thread geometry parameter and applies all above research results to quantificationally measure geometry parameters such as half angle of thread, coning and pitch of conical joint screw thread of graphite electrode, at last educes influencing expressions of the shelter of lead angle to half angle of thread change, it establishes theory base for error amendment.

论述了微小零件尺寸及形状误差的常用测量方法，提出了将视觉检测技术应用到微小零件尺寸及形状误差的测量中，构建了视觉检测系统的硬件结构，开发了相应的测量软件，应用第三章介绍的各种边缘检测方法对传动用滚子链链板进行了边缘检测对比实验，找出适合的边缘检测方法，并应用上述各章的研究结果对传动用滚子链链板节距以及圆形零件的圆度误差进行了测量。

Minimus density were collected from 1984 to 1993. Grey correlation analysis was carried out to study the relationship of 18 indices with An. minimus density. E variable was developed and its relation to the An. minimus density was analyzed to establish an evaluation model.

对18项指标与微小按蚊密度的关系进行灰色关联度分析，按照灰色阈值筛选评价指标，合成变量E，研究变量E与微小按蚊密度的关系，从而建立微小按蚊密度评价灰色模型。

In D3 gene with 5 site mutations. There exists individual variation in the COII gene, but this variation is less than that among other members of Myzomyia Series such as An. aconitus, An. jeyporiensis, etc. In addition, one haplotype different from both An. minimus A and C was found in these 3 genes studied.②The genetic distance between An. minimus A and An. minimus C is less than that among other members of Myzomyia Series. Molecular trees based on different genes and different methods show that An.

种系发生研究表明，微小按蚊复合体亲缘种A、C间的遗传距离远小于与之近缘的同系其它成员间的距离。2个分子标记3种方法构建的基因树显示，微小按蚊A、C的种系地位非常固定，不同地域来源、不同变异类型的微小按蚊始终在同一分"枝"上，与迈蚊系其它成员相比，呈现其相对稳固的单源性。

The total RNA was abstracted from the larvae of Boophilus microplus, and a 1982bp Bm86 gene was amplified by RT-PCR. The target gene was subcloned into T vector. The sequencing showed that the nucleotide sequence of the cloned Bm86 gene shared 97.4% homology with the data published in and this fragment contained the complete open reading frame of Bm86 gene.

为了克隆微小牛蜱Bm86 基因及构建该基因的表达载体，以微小牛蜱饥饿幼蜱的破解物提取的总RNA为模板，参照已发表的微小牛蜱Bm86基因的核苷酸序列，设计了1对引物，采用RT-PCR技术获得微小牛蜱Bm86基因；将Bm86基因克隆入载体，并进行序列分析，结果证明，克隆的Bm86基因序列与GenBank上登录的Bm86基因序列的同源性达97.4%，而且该序列包含完整的开放阅读框。

In D3 gene with 5 site mutations. There exists individual variation in the COII gene, but this variation is less than that among other members of Myzomyia Series such as An. aconitus, An. jeyporiensis, etc. In addition, one haplotype different from both An. minimus A and C was found in these 3 genes studied.②The genetic distance between An. minimus A and An. minimus C is less than that among other members of Myzomyia Series. Molecular trees based on different genes and different methods show that An.

种系发生研究表明，微小按蚊复合体亲缘种A、C间的遗传距离远小于与之近缘的同系其它成员间的距离。2个分子标记3种方法构建的基因树显示，微小按蚊A、C的种系地位非常固定，不同地域来源、不同变异类型的微小按蚊始终在同一分&枝&上，与迈蚊系其它成员相比，呈现其相对稳固的单源性。

OBJECTIVE ①To compare the sequences of the ribosomal rDNA-ITS2, 28S-D3 and mitochondrial mtDNA-COII in the different origins of Anopheles minimus for detecting the intra-and inter-specific variation of the sibling species of the Minimus Complex;②To reconstruct phylogenetic trees of the Minimus Complex and Myzomyia Series for determining the position of An.

目的 ①比较微小按蚊核糖体rDNA-ITS2、28S-D3基因和线粒体mtDNA-COII基因的序列差异，阐明微小按蚊复合体亲缘种种间和种内变异；②根据基因变异重建微小按蚊复合体及迈蚊系成员的种系发生关系，以确立微小按蚊亲缘种变异的种系地位；③建立微小按蚊复合体亲缘种A和C的分子鉴别方法。

OBJECTIVE ①To compare the sequences of the ribosomal rDNA-ITS2, 28S-D3 and mitochondrial mtDNA-COII in the different origins of Anopheles minimus for detecting the intra-and inter-specific variation of the sibling species of the Minimus Complex;②To reconstruct phylogenetic trees of the Minimus Complex and Myzomyia Series for determining the position of An. minimus A and C;③To establish specific methods for differentiation of two sibling species, An. minimus A and An. minimus C, of the Minimus Complex.

中文题名微小按蚊种群变异与分子鉴别研究副题名外文题名论文作者周水森导师汤林华研究员学科专业分子流行病学研究领域\研究方向学位级别博士学位授予单位中国预防医学科学院学位授予日期2002 论文页码总数98页关键词蚊微小按蚊馆藏号BSLW /2003 /R384 /3 目的①比较微小按蚊核糖体rDNA-ITS2、28S-D3基因和线粒体mtDNA-COII基因的序列差异，阐明微小按蚊复合体亲缘种种间和种内变异；②根据基因变异重建微小按蚊复合体及迈蚊系成员的种系发生关系，以确立微小按蚊亲缘种变异的种系地位；③建立微小按蚊复合体亲缘种A和C的分子鉴别方法。

A new picorna-like virus was isolated from dead Evtropis oblique larvae deceased from NPV infection. Electron microscopic observations of purified virions were non-enveloped isometric particles with diameter of 26nm. The virions contain two capsid proteins: 31.5kDa and 28.8kDa, the amount of later is 2.5 times more than that of the former. Analysis of 3′terminal sequence of EoPV clone identified that it can encode RdRp and has eight conserved motifs. Homology analysis shows that it is closely related to Perina nuda picorna-like virus.

从核型多角体病毒感染致死的茶尺蠖幼虫尸体中分离到一株微小RNA病毒透射电镜观察纯化的病毒粒子为无囊膜、无表面特征、直径约26nm的球状颗粒。16%的SDS-PAGE显示它有两个分子量为31.5 kDa和28.8 kDa的衣壳蛋白，后者的含量是前者的2.5倍。3′端克隆序列分析表明EoPV基因组3′有poly尾，编码RNA聚合酶，含有微小RNA病毒RNA聚合酶的八个保守基序，同源性分析表明它与榕透翅毒蛾微小RNA病毒亲缘关系最近。

Results We found that in all individuals'blood there are an amount of leucocytes parasitized by some moving-ac-tively tiny organisms,which even were found in some of the individuals'blood platelets.After dying we had observed the extracellular fluid of leucocytes and blood platelets.Analogically,in some plant juice and tissue cells we had also found the similar tiny organism,which had an ability to survive under the heat of90℃for30minutes.

结果 观察发现所有人血液中都能找到不同百分比的白细胞内有运动活泼的微小生物寄生，部分人血小板内也有，染色后还能观察到白细胞和血小板的外浆，在部分植物的汁液和细胞中也能看到类似的微小生物，这种微小生物还能耐受90℃高温30min以上。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。