待用

Methods The skeletal muscle stem cells were enzymatically isolated with collagen and dispase, and then purified by different adhesion time from 6 female SD rats. The purified cells were transinfected with adenovirus carrying lac-Z gene, and injected into the bladder neck of 6 autologous and 4 allogeneic rats. Tissues of blader neck and ureter were obtained in sacrified rats after 5 and 15 days, histological examination and X-gal staining were performed.

采用混合酶消化及反复差速贴壁法分离、纯化培养SD大鼠骨骼肌干细胞，然后用携带lac-Z基因的腺病毒载体转染肌干细胞，待转染成功后将携带lac-Z基因的骨骼肌干细胞注射于自体和同种异体的膀胱颈及后尿道周围，分别于注射后5、15d，处死大鼠取出膀胱颈及后尿道，行组织学检查及X-gal染色。

The diameter is 15.5 centimetres, 8.5 centimetre high, is it make various kinds of flowers line kind to carve gold and silver thin slice , paste in painting childing smooth of polishing with paint, after being dry, overall Xiu several of celluloid paint is it show the honeysuckle line to grind and then, make pattern and paint bottom reach same Pingdu , push away light become a exquisite one flat to take off the lacquer ware in addition.

直径15.5厘米，高8.5厘米，将金银薄片刻制成各种花卉纹样，用漆粘贴在打磨光滑的漆胎上，待干燥后，全面髹透明漆数层再经研磨显出金银花纹，使花纹与漆底达到同样平度，再加推光则成为精美的平脱漆器。

After cooling, the excess hydride was destroyed by the dropwise addition of IPA.

待冷却后，用外加物异丙醇滴定多余的氢化物。

Add system usually pumped into a sealed container on high vacuum, and then gastightness leak detection, leak detection to be passed, with the addition pump container Wang added, add it to reach the requirements.

加注系统通常先对密封容器抽成高真空，然后进行气密性检漏，待检漏通过后，用加注泵王容器中加注，使其迅速达到加注的要求。

Software:LightKeeper backlight is used to control the mobile phone software You can control the screen backlight pleases, and the backlight to avoid over-stimulation of the eyes, and can save electricity to allow a longer waiting time.

软件：LightKeeper背光是用来控制手机软件，你可以控制屏幕背光为所欲为，背光，以避免过度刺激眼睛，并能有效节约电量，让待机时间更长。

When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added , kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2－4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L－1 after their number counted.

选取2～5代的小鼠胚胎成纤维细胞，待其至对数生长期倒掉或吸掉培养基，加入含10 mg/L丝裂霉素C的细胞全培养液5 mL，置37 ℃，体积分数0.05的CO2饱和湿度温箱中培养二三小时，在6孔板中加入0.1%明胶包被，放置30 min以上，倒掉或吸掉含丝裂霉素C的培养基，磷酸盐缓冲液清洗5遍，尽量除去丝裂霉素C，加入2 mL 0.05%的胰蛋白酶消化细胞，镜下观察，当细胞间出现裂隙，细胞变圆时（约2～4 min），立即加入等量的全培养液终止消化，并用吸管反复吹打，使之成为单细胞悬液，在细胞计数板中央放置专用的盖玻片，用玻璃虹吸管吸取细胞，让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液，至盖玻片下被液体充满为止。

The underlying diseases included chronic glomerulonephritis(in 71 patients),nephrosclerosis(in 30),diabetic nephropathy(in 5),polycystic kidney disease(in 3),and miscellaneous or unknown disorders(in 9).The severity of renal insufficiency was classified under two groups according to the base-line creatinine clearance:32 patients had mild insufficiency (Ccr 46～60 ml/min),and 86 had moderate insufficiency (Ccr 30～45 ml/min).

先依次选用倍他乐克、双氢克尿噻、心痛定控制病人血压，待血压及肾功能稳定后，根据Ccr将病人分为轻度CRF组(Ccr 46～60ml/min)和中度CRF组(Ccr 30～45ml/min)，每组病人再随机分为治疗组（抑平舒2.5～5.0 mg/qd n=80）和对照组（n=38），所有高血压病人继用上述降压药维持正常血压，随访期6个月。

According to the number of unknown variables in ideal element fitting, variable substitution is adopted to convert the overdetermined nonlinear equations into overdetermined linear equations. Cumulative sum operator is used to solve the overdetermined linear equations.

根据理想要素拟合过程中待求未知量的个数，运用变量代换，将非线性超定方程组转化为线性超定方程组；对变换后的超定线性方程组施加累积算子，进行未知数求解；最后用仿真实例进行了分析，并与CIRP方法进行比较。

Chapter 4 'A Summary of Zhuang Zi's Poetic Thought', based on a'close reading'of thirty-three sections of Zhang Zi and the three parts'primordial thought'T's discourse'and'poetic living', systematically combs and interprets the basic framework of Zhuang Zi's poetic thought.lt starts from a'primordial thought' about a 'carefree life' that overcomes 'grief and 'heavy burdens'and obtains Toe's wisdom', and experiences a judgment of various discourses of man, a listening to the discourse of heaven and earth and a preception of 'Tao's discourse'. With this as a basis, a realm of 'poetic living' is attained: such a realm is rooted in a confirmation of 'useless use', a method of 'inaction',and an ideal state of 'floating by means of Tao-Te'.

第四章&庄子诗学思想述略&，主要通过对现存三十三篇的《庄子》的&文本细读&，围绕&本然之思&、&大道之言&、&诗化人生&三大板块，系统清理和阐释庄子诗学思想的基本框架：它从对于充满太多&悲恸&、&无奈&、&生存重负&的&荒诞人生&和与&体道智慧&相联系的可能的&自在人生&的&本然之思&出发，经由对各种&人言&的分辨、对&天地之言&的聆听、对&大道之言&的感领，最后通达逍遥无待的&诗化人生&之境——种植根于&无用之用&的确认，借助于&无为而观&的手段，通过&乘道德而浮游&的体验而通达的理想人生境界。

The c oaventioaal method was improved by using agarose as supporting medium instead pured agar and adopting pre-electrophoresis in order to make it more sensitive.

笔者在常规方法的基础上，做了些改进：用琼脂糖代替纯化琼脂；并先将诊断血清加在阳极端孔内，预电泳15—20分钟，然后将待检标本加入阴极端孔内继续电泳50分钟。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新