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Methods 24 SD rats were randomly divided into normal group,model group,aminophylline treatment group(35 mg/kg), 8 rats in each group. The bronchial asthma model in rats was built by egg protein sensibilization and longterm inhalation provocation. The rats in treatment group were lavaged each day from the first time of provocation.After 4week treatment, the rats were killed to obtain lung tissue for staining with HE.

24只SD大鼠随机分为正常组、模型组、治疗组,每组8只,除正常组外以卵蛋白致敏并吸入激发法制备大鼠哮喘模型,治疗组、模型组从第1次哮喘激发开始(造模第3周)分别给予氨茶碱、0.9%氯化钠溶液灌胃给药,1次/d,用药4周处死大鼠,取肺组织HE染色,用彩色图像分析仪测量支气管壁面积、支气管平滑肌面积,用ELISA双抗体夹心法测定肺组织MMP9和TIMP1含量。

The bronchial asthmatic model was established by egg protein sensibilization and longterm inhalation provocation.The rats of each treatment groups were lavaged each day from the first time of provocation to execution.After 4 weeks of treatment,the rats were killed to obtain lung tissue for dyeing of HE.A computer assisted image analysis system was used to determine the thickness change of bronchus wall and smooth muscle.The contents of MMP9 and TIMP1 in lung tissue were determined by ELISA double antibody sandwich method.

除正常组外以卵蛋白致敏并吸入激发法制备大鼠支气管哮喘模型,各治疗组均从第1次哮喘激发开始(造模第3周)至处死前每天灌胃给药,激发并给药4 w后处死大鼠,取肺组织HE染色,彩色图像分析仪测量支气管管壁厚度、平滑肌厚度,采用ELISA双抗体夹心法测定肺组织MMP9、TIMP1含量。

Methods: Eighteen SD rats were randomly divided into A,B,C groups. A,B groups were induced to RE by operation, C group had false abdominal operation. All rats were killed 2-3 months later and the NO,VIP neurons were investigated qualitatively and quantitatively by immunohistochemical method and color image analyzer.

18只SD大鼠随机分为A、B、C3组,A、B两组手术造成RE,C组采用伪开腹术。2~3个月时用免疫组化法及彩色图像分析仪对3组大鼠食管组织中的NO及VIP含量进行定性、定量测量。

Hospital Management Computer networking implementation Informationization and have Siemens 1.5t large superconducting magnetic resonance imaging systems, Philippe's spiral ct, gastrointestinal shimadzu 800mA remote machine, large-scale digital subtraction imaging system, Hitachi large-scale chemical and biological detectors companies, blood gas analyzer, hd13000 color Doppler ultrasound diagnostic apparatus, Varian linear accelerator and the United States conformal radiotherapy planning system, etc. A large number of modern-art medical equipment.

医院管理实现微机联网信息化,并拥有西门子公司的1.5t大型超导磁共振成像系统,菲利蒲公司的螺旋ct、岛津800ma遥控胃肠机,大型数字减影成像系统、日立公司大型生化检测仪、血气分析仪、hd13000彩色多普勒超声诊断仪、美国瓦利安直线加速器和适形放疗计划系统等一大批现代化的先进医疗设备。

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