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形成原纤维

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To explore the potential mechanism of cigarette smoke extract -induced cell injury, primary cultured skin keratinocytes and fibroblasts from rat were selected as target cells in Part Ⅰ study compared with the effect of UVA.

本研究第一部分以原代培养的大鼠皮肤角质形成细胞和成纤维细胞为对象,采用UVA损伤作用为参照,探索香烟雾提取物(Cigarette SmokeExtract,CSE)对皮肤细胞的损伤作用,重点观察CSE所致的皮肤细胞毒作用、功能损伤及可能作用机制。

The histological findings of the primary tumor and metastatic lymph nodes demonstrated massive cancer cell degeneration such as pycnosis and vacuolation, xanthogranulomatous inflammation and dense fibrosis. The effect of NAC was judged to be grade 2 histologically.

组织学检查发现肿瘤原发灶和转移的淋巴结癌细胞大量退化如核固缩和空泡形成,黄肉芽肿炎以及密集的纤维化,经组织学判定NAC的疗效为2级。

Another six specimens of each group was obtained for biomechanical test at 8 weeks. RESULTS: There were no significant differences between control group and fibrin group. In bFGF group compared with control and fibrin groups, appearance of the angiogenesis, fibroblast proliferation and collagen production in the sheath, epitenon and parenchyma at repair site occurred earlier and were more in quantity, the gliding excursion of the tendon was shorter, and the work of flexion and the ultimate tensile strength of the tendon were greater.

结果:对照组与纤维蛋白封闭剂组间各项观测指标比较均无显著性差别,与对照组和纤维蛋白封闭剂组相比,bFGF组修复部位腱鞘、腱外膜及腱实质的新生血管形成、成纤维细胞增殖较好,胶原的分泌出现早,数量也较多;肌腱滑动距离较短,屈曲功和肌腱最大抗拉力较大。

Many factors may be involve in the course. To investigate the regulation activity of mesenchymal cells to differentiation of epithelial cells from hair follicle and to study its differentiation property, mesenchymal cells gel was made by nubby dermal papilla cells, free dermal papilla cells, skin fibroblasts. Skin keratinocytes and epithelial cells from hair follicle were inoculated on the gel surface and cultured in air-liquid interface. Three-dimensional model of DPC using to induce epithelial cells differentiation is built in vitro.

为了进一步研究毛囊细胞间的相互作用,探讨毛囊间质细胞对毛囊上皮细胞分化的调节作用,研究毛囊上皮细胞的分化特性,我们利用团块状的毛乳头细胞,游离分散的毛乳头细胞或皮肤成纤维细胞制成间质细胞胶原凝胶,表面接种皮肤角质形成细胞或毛囊上皮细胞,进行气-液界面培养,在体外建立了毛乳头细胞诱导毛囊上皮细胞分化的立体模型。

With the increase of cell density, soma was slenderer and slenderer and like fibroblast.② Growth curves of PDMCs: Growth latency ranged from 2 to 8 days after cell inoculation. During this period, adherence was observed gradually but not obviously amplified. Eight days later, cells entered log growth phase.

结果:①多分化潜能细胞的原代培养情况:胎盘组织消化后获得细胞中仅有少量的贴壁细胞,经两周后逐渐形成扁平单层细胞,呈漩涡状生长或成簇生长,随着细胞密度的增加,胞体变得细长,形态类似成纤维细胞。

Formalin-fixed and paraffin-embed-ed samples from 40 infant hemangiomas of glandulae parotid,30hemangioma cuits and 5 normal glandulae parotid tissues werestudied in immunohistochemical SP method.

Ⅳ型胶原、层粘连蛋白和纤维连接蛋白在腮腺血管瘤中均呈高表达;bFGF与ECM存在着一定的正相关关系,表明它们在腮腺血管瘤的形成过程中起着不可忽视的作用。

These results suggest that curcumine can prevent hepatic fibrosis via inhibiting proliferation and collagen I production of HSCs, which may be partly related to its regulative effect on the expression of NHE mRNA gene.

姜黄素可能通过调控肝星状细胞NHE-1 mRNA的表达来抑制HSC的增殖及Ⅰ型胶原的合成等机制,从而抑制肝纤维化的形成。

In the exudates from wounds, IL-1 was detected. IL-1 is capable of promoting proliferation of fibroblasts and excretion of collagenase, which induces inflammation and fever and promote wound healing. Inflammation is a defense reaction of the body.

同时,在局部创伤渗出液中测出含有IL-1,而IL-1可促进纤维细胞增殖及促进分泌胶原酶,引起炎症和发热,促进创面愈合,而炎症反应被认为是机体的防御反应,因此MEBO具有抗感染、止痛、促进创面愈合和减轻瘢痕形成等作用。

Marston, MD, from the University of North Carolina School of Medicine in Chapel Hill, and colleagues."Dermagraft is a bioengineered dermal substitute that laboratory data suggest has two principal modes of action. It provides living, human dermal fibroblasts that deposit matrix proteins and facilitate angiogenesis. It also provides a preformed collagen matrix, receptors, and bound growth factors that facilitate the migration of the patients' epithelial cells that close the wound."

Marston医师及同事表示,针对治疗慢性溃疡的生理失能的患者,还需要更多有效的疗法,而人工皮肤就是一种生物工程皮肤代用品,这一项实验室数据显示两种最重要的功能模式,它提供活体人类皮肤纤维组织母细胞,储存母体蛋白质并促进血管新生,另外它也提供预先形成的胶原母体、接受器及束缚成长因子,帮助病患的上皮细胞移动,使伤口愈合。

RG has an inhibitory effect on the formation of liver fibrosis and cirrhosis. Its mechanism may be related to the improvement of hepatic function, decrease of serum TGF β1, collagen Ⅰ and Ⅲ and Hyp levels.

结论]软肝颗粒能改善肝组织病理变化,恢复肝功能,降低血清TGFβ1和Ⅰ、Ⅲ型胶原水平及Hyp含量,抑制肝纤维化、肝硬化的形成。

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