形成原纤维

aim to study the efficaciousness of wuling cap-sules for hepatic fibrosis in rats.methods rat models with hepatic fibrosis were induced by ccl4 compound factors and then treated with wuling capsules.pathology of liver sections,hyaluronic acid and procollagencontents in rat liver and serum were examined respectively.results pathological examinations showed that the liver cells of mod-el rats were injured seriously.collagen fibers proliferated and dissected hepatic lobules in model rats.wuling capsules could protect liver cells and reduce degrees of hepatic fibro-sis.the best effects were achieved at3.0g?

目的 研究五灵胶囊对大鼠肝纤维化的作用。方法采用以四氯化碳为主的复合因素致大鼠肝纤维化，观察五灵胶囊对肝组织病理学、肝组织和血清中羟脯氨酸、ⅰ，ⅲ型前胶原含量的影响。结果病理学检查显示，模型组大鼠肝细胞损伤严重，胶原纤维大量增生，形成假小叶。五灵胶囊组大鼠肝细胞结构破坏不明显，肝纤维化程度轻，在3.0g？kg-1 时治疗效果最好。

Antibodies against col Ⅲ reacted strongly in the entire pterygial stroma. Conclusions Components of ECM are changed in the pterygium: the sub-epithelial connective tissues and vessels are more prominent in the pterygia than in normal conjunctiva tissues, and abnormal fibers are visible only in the deeper subepithelial connective tissues. This study documents the overexpression of LN and col Ⅲ. They may play roles in cellular adherency, migration and angiogenesis. Also this study documents the overexpression of MMP3 which may degrade ECM in the pathogenesis of the pterygium.

结论翼状胬肉组织中ECM的成分较正常球结膜发生改变，有大量的弹性纤维变性、胶原纤维增生及新生血管形成；col Ⅲ在翼状胬肉组织中表达增加，LN及胶原纤维可能对翼状胬肉中血管形成、细胞粘附和迁移及ECM重建起作用；MMP-3在翼状胬肉组织中表达增加，其对ECM成分的降解作用可能有助于翼状胬肉的形成和向角膜中央侵袭。

Electron microscopic morphometry of heart samples revealed typical diabetic alterations consisting that myofibril arranged disordly, mitochondril swelling, mitochondrial crista and breaking and solving to form the lysis of cistae; some glycogen and lipid droplet depositing in myofibril.

模型组心肌细胞肌原纤维排列紊乱、断裂、溶解，线粒体肿胀变性，线粒体嵴断裂、溶解，形成空白区，肌细胞中可见糖原脂滴沉着；与模型组比较，TXS组心肌细胞肌原纤维排列整齐，线粒体形态尚可，偶有线粒体外膜缺损。

Results :①The MVs were formed by osteoblast of the secret type、dentinoblast and process;② There was a mature process of the MVs in the extracellular matrix.The appearance of the hydroxyapatite was a major mature feature;③ HAP formed initially in the MVs entered into collage matrix and eventually mineralized;④ The shifting sign of HAP from MVs to the surface of collage fibril was observed.

结果：①MVs是由分泌型成骨细胞、成牙本质细胞及其突起芽生进入胶原基质；②MVs在细胞外胶原基质中有一个逐渐成熟的过程，并以羟基磷灰石（hydroxyapatite，HAP）晶体的出现为其主要特征；③HAP在MVs内形成、增多，最终进入胶原基质并进一步沉积、钙化；④部分胶原原纤维表面沉积的HAP 晶体与MVs分泌的晶体有明显的移形迹象。

Special staining methods, such as Masson and the Van Gieson staining were used to study the distribution of collogen fibers and elastic fibers. ResultsBy HE staining, the subepithelial connective tissues and vessels in the pterygium were more prominent than normal conjunctival tissues. An amorphous subepithelial superficial hyalinized zone and coarse eosinophilic granular materials were observed in the pterygia, but they were not found in normal conjunctival specimens. Coarse fibers were visible only in the deeper subepithelial connective tissues of pterygial samples. With Masson′s staining, the dense staining of collagen fibers was also more prominent in the pterygium than in the subepithelial connective tissues of normal conjunctiva. Abnormal collagen fibers were visible in the deeper sub-epithelial connective tissues of pterygial samples. With Van Gieson staining, abnormal collagen fibers were visible in the deeper subepithelial connective tissues. Dark coarse elastic fibers were found in the abnormal fibers only in the subepithelial deep connective tissues of pinguecula in the pterygia but not in the conjunctiva. With immunohistochemistry staining, MMP-3 was strong in the pterygial epithelium, moderate in fibroblast and absent from pterygial vascular walls. LN was strongly expressed in the blood vessel wall, moderately in the epithelial basement membrane and absent from the entire stroma.

结果HE染色：翼状胬肉组织上皮下基质中存在结缔组织的增生和血管形成；基质浅层存在一无定形物质透明区及粗糙的颗粒样嗜酸性物质，在翼状胬肉体部深层基质中存在粗糙的纤维组织；正常球结膜组织细胞排列整齐；基质为疏松结缔组织，胶原纤维平行排列，其间可见成纤维细胞，散在少量中性粒细胞、毛细血管；Masson染色：翼状胬肉浅层基质中存在致密的胶原纤维染色，深层基质中的胶原纤维存在变性样改变；VG染色：翼状胬肉组织深层基质中存在大量变性的胶原纤维，其间夹杂黑色的弹性纤维；免疫组化染色法：MMP-3在翼状胬肉上皮细胞中呈强表达，成纤维细胞中呈中等强度表达，血管内皮细胞中未见表达；LN在血管壁中呈强表达，在上皮细胞基底膜中呈中等强度表达，在整个基质中未见明显表达；col Ⅲ在整个翼状胬肉基质中呈强表达。

The psammoma body mineralization in meningioma is a common type of mineralizationThe analysis of the mineral composition may provide some support information in finding the reason of happening and developing of the diseaseThis paper focuses on the concentric layered structure mineralization in meningiomas, using mineralogical methods, such as HRSEM, ESEM, EDAX, EPMA, HRTEM, XRD and FTIR to systematically investigate the mineral composition, structure and shape of the minerals in psammoma bodies in meningiomasWe have devised a method for preparing the silicon wafer sheet which was used for the ESEM insitu observations and analysisIn this study, we first got the ESEM and HRTEM images of the initial mineralization phase of meningiomasThese images showed that in the early stage of psammoma body mineralization in meningiomas, many mineralized balls composed of octocaphosphate were precipitated on the collagen fibersThese balls continued to grow and aggregate, and were gradually hydrolyzed to become the dahlliteThe continued development of mineralization resulted in the mineralized collagen fibersThe study revealed that the concentric layered structure of the psammoma bodies in meningiomas is formed by the spiral arrangement of the mineralized collagen fibers on which the mineralized grains precipitated.

砂粒体矿化是脑膜瘤中常见的矿化类型，对其形成机理和矿物成分的分析可能会对肿瘤发生、发展的研究提供辅助信息。该研究选取人脑膜瘤中的砂粒体矿化作为研究对象，采用偏光显微镜、环境扫描电镜及能谱、X射线衍射仪、高分辨透射电镜和电子探针对样品的形貌、结构和成分进行测试分析，并以此为依据探讨脑膜瘤中砂粒体的形成机理。研究结果表明矿化的初期为沉淀在胶原纤维上的矿化小球，成分为磷酸八钙；矿化小球不断生长聚集，并逐步水解为碳羟磷灰石晶体，矿化的不断发展致使胶原纤维也发生矿化。砂粒体的同心层状构造是由螺旋状排列的矿化胶原纤维及沉淀在其上的矿化颗粒组成的集合体，而不是多数研究中所述：砂粒体是以坏死细胞残骸为中心由内至外的同心层沉淀。

Methods Constructed the model of fetal scarless wound healing and investigated the expression of collagen in fetal and adult rat wounds by immunohistochemistry and in situ hybridization.

染色，Ⅰ、Ⅲ型胶原免疫组化，Ⅰ、Ⅲ型胶原原位杂交。结果胎鼠伤口愈合快，组织结构再生完全，没有瘢痕形成，胶原呈网状正常排列，Ⅰ、Ⅲ型胶原蛋白及表达均增多，同时伴细胞数增多，Ⅲ型胶原首先形成网状排列；成年伤口胶原纤维沉积慢，平行排列的胶原逐渐呈束状填塞伤口以致瘢痕形成，伤口内Ⅰ、Ⅲ型前胶原mRNA表达均增多，细胞数目增多不明显。

On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明，所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达；在缺乏AMBN情况下，与对照组相比，实验组牙胚在体外可以继续生长发育至钟状晚期，出现成釉细胞和成牙本质细胞的分化，成釉细胞可以分化成为分泌期型成釉细胞，胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体，缺乏溶酶体，表明对蛋白合成和脚的能力降低；实验组牙胚有牙尖形成和基质分泌，但牙尖形态异常，基质形成减少，牙尖周围基质最厚处为O.6卜m，明显薄于对照组的5.spin，基质中胶原纤维粗细不等，排列稀疏， 3 第四军医大学硕士学位论文未见钙化现象，充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程，影响胶原纤维和牙本质基质的合成，促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

Act or process of forming fibrils .

形成原纤维的动作或过程。

This study was conducted to examiune the fibrotic effect of Ni-Ti and 317L al loys in esophagus.The extract fluid from Ni-Ti,317L alloys was made according t o the ASTM standards of U.S.A. The Fb of esophageal scar was cultured primarily ,then incubated with alloy abstract fluid. The proliferating activity of Fb was measured by MTT at 4, 24, 48, 72 hours in the course of culturing. The esophagu s embedding test of Ni-Ti,317L alloys was made according to ASTM standards of U .S.A.The tissue around the alloys was taken at weeks 2 and 12,and the pathologi c changes were analysed.

为探讨新型支架材料Ni-Ti、317L合金在食管局部的致纤维化作用，按美国ASTM标准制备NiTi、317L合金的金属浸提液；&组织块培养法&原代培养食管壁疤痕的成纤维细胞，传代后以金属浸提液进行培养，分组后分别培养4、24、48、72 h，MTT法检测不同培养时间后Fb增殖功能的变化；按美国ASTM标准进行NiTi、317L合金试件的食管壁内包埋实验，即将金属试件经表面处理后直接置入食管壁粘膜层与肌层之间，术后2、12周取出包埋组织，分析试件周围组织的病理变化，并进行胶原纤维染色，观察纤维形成状况。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。