形态形成的

GFP gene eukaryotic express plasmids were transfected into ES cells. The cells were observed under the inverted fluorescent microscope. The clones which expressed the powerful green fluorescence were chosen to be named ES-GFP cells and cultured continuously. The examination of ES cell totipotent including Alkine phosphatase staining, embryonic body formation in vitro and teratomas formation in nude mice was implemented.

1。采用脂质体方法将GFP质粒转染ES-D3细胞株，经筛选后倒置荧光显微镜观察细胞形态及荧光状态，挑取荧光最强的克隆，命名为ES-GFP细胞，进一步扩增培养，观察细胞生长及荧光表达情况，行细胞分化全能性鉴定包括碱性磷酸酶（alkine Phosphtase，AKP）染色、体外胚胎体（embryonic body，EB）形成实验及裸鼠体内成瘤实验。2。

This paper mainly studies on people Jui-Tu neglected to look further into these questions.:one, through the works of Zhang Rui Figure comprehensive carding, so can their have a comprehensive understanding; 2, the morphological changes from the works start, to explore its origins calligraphy; 3, through their works shall know Handscroll analysis to help determine without the banners section in the approximate age of the work; 4 of their book to learn the concept of analysis, in order to more clearly understand the formation process of calligraphy styles. 5. Allograph home and at the same time learn the concept of the book, we can know that the formation of personality style and its concept of an independent study of the book is closely related to, but also deepen their understanding of the law of Zhang Rui.'s calligraphy.

本文主要就人们研究张瑞图时所忽略的一些问题做进一步的探讨：1、通过对张瑞图作品的全面梳理，以求能对其有一个全面的认识；2、从其作品的形态变化入手，探寻其书法的渊源，并尝试对其书法上一个很长的空白期进行探索；3、通过对其手卷作品进行款识分析，以帮助对无年款的条幅作品进行断代；4、对其书法观念进行论述，以求更清晰地理解其书法风格的形成；5、与同时代书家书法观念的比较，可以知道个性风格的形成与其独立的书学观念密切相关，更能加深对张瑞图书法的理解。

New bone formation was observed to be increasing significamly in both DCN bone cements and DC bone cements with the passage of time. By the end of 24 weeks, new bone had bridged the gap between the proximal and distal fragments in DCN bone cements and DC bone cements. By histomorphological detection in DCN bone cements, Abundant of chondral and new bone islands were seen at the site of defects 4 weeks after the operation and a number of new bones formed and enlarged by both enchondral ossification and appositional formation by the 8th weeks.

研究发现，DCN骨水泥植入4周大量间充质细胞分化，在材料与骨端之间出现一层软骨细胞，软骨细胞继续增生并向编织骨分化；8周材料降解的同时，小梁骨出现；12周新骨长入材料并已经基本替代完成，形成初步骨性连接；24周骨缺损完成骨性连接，新骨进行结构改建，材料内部形态结构明显变化。

Through the knowledge of the urban societies, this case study puts the New Caoya Urban Squatter Settlements back in the Story of the urbanization and the housing policy, discuss its historic and social formation, breaks the public opinion's prejudice, puts in light the ill-assorted sharing of the country resources and the unequal development of the cities.At the same time, this research introduces the formation and the structure of the New Caoya Urban Squatter Settlements - it comes at first from the urban development bringing a need of workers, the population migrates massively from the countryside to the city, the study shows more clearly the bad regulation of the housing policy. In the time, the authorities, to relieve the tension of the consumption, let the Squatter Settlements form. With the evolution of the urbanization, it became a choice of extra housing. Today the New Caoya Squatter Settlements play still an important role among the low incomes families.

本研究透过都市社会学认识论的仲介，将新草衙违建聚落放回到都市发展与住宅政策的历史脉络中，探讨新草衙违建聚落的形成历史及其社会过程，破除社会大众对新草衙违建聚落边缘性的偏见，还原新草衙违建聚落背后所隐含的国家资源分配不均及城乡发展不均衡等意涵，同时透过新草衙违建聚落的研究，指出新草衙都市违建聚落的形构，首先由都市产业发展带动劳工需求，乡村大量人口涌进都市，进而为都市土地住宅政策调节失当所深化，而当时国家或都市当局为缓和都市集体消费问题的压力，默许放纵此违建聚落的形成，而随著都市发展的演变，作为非正式部门住宅服务的特殊形态，新草衙违建聚落至今仍担任著提供中低收入住宅的重要功能。

During the four years study on the project, we develop a dynamical forecast model that describing the subtropical ridge surface. A new theory on the role of the land-sea thermal difference on the formation of the Subtropical High is advanced. It is concluded that in the summer subtropics over each continent and its adjacent ocean LO, SE, CO, and a double-dominant heating from west to east compose a LOSECOD heating quadruplet. The influence of the Eurasia continent on SH varies according to the seasonal variation. From the ideal numerical simulation it is shown the important role of the Eaiuan continent and the huge topography on the mechanism of formation and mantanence of the SH during the summer. It is verified by the numerical simulation that the extension of the subtropical continent into the tropics greatly enhances the " East Asian monsoon". Through the Rossby energy dissipation theory it is verified that the deep latent convective heating induces the onset of the South China Sea monsoon over the Bay of Bengal. The relationship and mechanism between the precipitaiton over the East Asia and the Subtropicla high is illustrated by the data. It is also found the close phase lock between the east-west shift of the SH and the disturbence of the east wind belt over the tropical troposphere. The interaction between the structure and activity feature of the SH and the Meiyu over the Yangtze-Huaihe river valley is revealed from the data. The numerical simulation verified the impact of the different SH pattern on the path of the typhoon.

本项目的研究发展了描述副热带高压脊面变化的动力模型，提出了夏季副热带地区四叶型非绝热加热分布型及相应环流拼图的创新理论；明确了大陆尺度的海陆热力差异在副热带高压形成中的作用；指出欧亚地形对副热带高压和气候的影响随季节变化而变化，通过数值试验证明春季青藏高原的感热加热对亚洲夏季风的爆发地点起了"锚定"的作用，进一步证实了副热带高压断裂和亚洲夏季风的最早爆发地点发生在孟加拉湾东岸；利用Rossby波能量频散理论证明了孟加拉湾深对流潜热释放诱导南海季风爆发的机制；揭示了东亚季风降水的季节内变化与西太副高的关系及机理；揭示了西太副高与东西风带中扰动之间的相互作用过程，指出西太副高的东西活动与热带对流层上空东风带扰动和中纬度西风带扰动的移动和锁相密切相关；研究了夏季淮河流域梅雨期间副高结构和活动特征及其与江淮暴雨的相互作用；验证了不同副热带高压形态对台风路径的不同影响；通过数值试验证明了欧亚大陆和青藏高原等大地形对副热带高压形成和维持的重要作用。

Result 1 Magnetic nanoparticles, magnetic nanoparticles modified with antisense oligodeoxynucleotide of human telomerase reverse transcriptase induced HL-60 tumor cells to apoptosis, we could see typical morphologic change of apoptosis cells: karyopyknosis, chromation"s condensing and aggregation in nuclear, forming crescent-shaped or annulus structures to lean on edge of cell nucleus"s membrane and posing apoptosis body by Atomic Force Microscope, Fluorescence microscope, transmission electron Microscope 2 There was a significant difference compared with control group(p.01), inhibition ratio had significant positive correlation with medication dosage and time ;during 0.8-8μM dosage amplitude, inhibition ratio accrescenced by dosages increasing. However, the inhibition ratio would decrease when dosage over 8-80μM.

结果 1 磁性纳米粒子、修饰有端粒酶反义寡核苷酸的磁性纳米粒子诱导HL-60细胞发生凋亡，原子力显微镜、光学显微镜、荧光显微镜和透射电镜下均观察到HL-60细胞呈现典型的凋亡细胞的形态变化：细胞核固缩，核内染色质浓缩、凝聚、形成新月形或环状结构紧靠在细胞核膜边缘，并形成凋亡小体。2 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子对HL-60肿瘤细胞的生长和增殖有明显的抑制作用，与对照组相比有显著性差异(p＜0.01)，在剂量为0.8-8μmol／L范围内，抑制率随剂量的增加而增加，当剂量超过8μmol／L时，抑制率反而下降；3 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子可增强p53基因的表达活性，引起DNA降解损伤，反向调节细胞周期活动，促使细胞从G0期进入G1期，抑制肿瘤细胞的生长。4 修饰有端粒酶反义寡聚脱氧核苷酸的量子点能通过内吞作用进入HL-60肿瘤细胞的细胞核，可以在细胞内进行定位和促进HL-60肿瘤细胞的凋亡。

The microphotography of the film was studied by scanning electron microscopy,which show that the film consists of different size of spherical particles,the bigger particles are dotted with smaller ones.The formation of the film consists of two steps : the formation and growth of the particles.

利用扫描电镜观察了膜的微观形态，膜是由大小不同的球状颗粒组成，大的颗粒之上夹杂或附着小的颗粒，膜的形成包含一个晶粒的形成和长大的过程。

In order to maximize change in the past vertical building structures, and overthrow the original shape influenced by it, these houses are anticlockly turned by 15 degree, supported and connected with 8 inclined steel columns. A core shaped-hollow construction is built to stand in the middle of this building to accomplish the rotating construction as the final formation. The outer curtain wall is hyperboloid metal net, which helps to form an overall rotation effects and well-protected the privacy of the residents.

为了最大限度的改变以往的垂直建筑结构，推翻在其影响下的建筑形态并满足面积为12*12的要求，我把每层的楼板按逆时针旋转15°并用8根钢柱连接，辅助斜向钢柱加以支撑，配合核心筒最终形成旋转的建筑结构，外表皮为双曲面金属网，能够有形成整体的旋转效果并很好的保护室内居民的隐私。

Smilax, there are the phylogenetic relationships and distributive pattern to be found based on the combined ITS and maiK. tree: 1 a clade with the African species (S. kraussiana) and Australian species (S. australis), being a sister group; 2 a clade with the S. zeylanica of sect. Macranthae and S. bona-nox of sect. China, the former from Southeast Asia and latter native to US, showing they are a sister species which is disjunctive between Asia-North America, similar to the species pair of S. hispida and S. sieboldii; 3 S. perfoliata from Indonesia being sister to S. ocreata of China and S. megacarpa of Indonesia, which are very similar to each other in morphology; 4 the three species from China (S. lanceifolia, S. perfoliata and S. chapaensis) and S.ovalifolia from Indonesia showing a 87-92% bts monophyletic clade; 5 S.bracteata from China and two species of Indonesia (S. leucophylla and S. sp.) forming a clade in the ITS tree; 6 S.

通过比较研究圆锥花序组和穗菝葜组的地理分布，基于ITS及matK联合数据构建的系统树发现下列明显的具系统发育关系的类群及地理分布模式：1来自非洲的S.kraussiana和来自澳大利亚的S.australis是一对姐妹群，具有共同的祖先；2圆锥花序组的S.zeylanica和菝葜组的S.bona-nox形成一个紧密的分支，前者分布于东亚而后者只局限于美国，显示了一种类似于S.sieboldii和S.hispida那样的东亚—北美间断分布模式；3印度尼西亚的S.perfoliata是分布于中国的S.ocreata和来自印尼S.megacarpa的姐妹群，这三者在形态上也非常接近，表明具有共同祖先；4来自中国的三个种(S.lanceifolia，S.perfoliata and S.chapanensis)和来自印尼的S.ovalifolia形成了一个高支持率的单系分支(87-92％bts)；5基于ITS的系统树，来自中国的S.bracteata和来自印度尼西亚的两个种S.leucophylla and S.sp。

During the four years study on the project, we develop a dynamical forecast model that describing the subtropical ridge surface. A new theory on the role of the land-sea thermal difference on the formation of the Subtropical High is advanced. It is concluded that in the summer subtropics over each continent and its adjacent ocean LO, SE, CO, and a double-dominant heating from west to east compose a LOSECOD heating quadruplet. The influence of the Eurasia continent on SH varies according to the seasonal variation. From the ideal numerical simulation it is shown the important role of the Eaiuan continent and the huge topography on the mechanism of formation and mantanence of the SH during the summer. It is verified by the numerical simulation that the extension of the subtropical continent into the tropics greatly enhances the " East Asian monsoon". Through the Rossby energy dissipation theory it is verified that the deep latent convective heating induces the onset of the South China Sea monsoon over the Bay of Bengal. The relationship and mechanism between the precipitaiton over the East Asia and the Subtropicla high is illustrated by the data. It is also found the close phase lock between the east-west shift of the SH and the disturbence of the east wind belt over the tropical troposphere. The interaction between the structure and activity feature of the SH and the Meiyu over the Yangtze-Huaihe river valley is revealed from the data. The numerical simulation verified the impact of the different SH pattern on the path of the typhoon.

本项目的研究发展了描述副热带高压脊面变化的动力模型，提出了夏季副热带地区四叶型非绝热加热分布型及相应环流拼图的创新理论；明确了大陆尺度的海陆热力差异在副热带高压形成中的作用；指出欧亚地形对副热带高压和气候的影响随季节变化而变化，通过数值试验证明春季青藏高原的感热加热对亚洲夏季风的爆发地点起了&锚定&的作用，进一步证实了副热带高压断裂和亚洲夏季风的最早爆发地点发生在孟加拉湾东岸；利用Rossby波能量频散理论证明了孟加拉湾深对流潜热释放诱导南海季风爆发的机制；揭示了东亚季风降水的季节内变化与西太副高的关系及机理；揭示了西太副高与东西风带中扰动之间的相互作用过程，指出西太副高的东西活动与热带对流层上空东风带扰动和中纬度西风带扰动的移动和锁相密切相关；研究了夏季淮河流域梅雨期间副高结构和活动特征及其与江淮暴雨的相互作用；验证了不同副热带高压形态对台风路径的不同影响；通过数值试验证明了欧亚大陆和青藏高原等大地形对副热带高压形成和维持的重要作用。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。