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A cDNA segment was isolated from M. meretrix larvae. The results of caspase in situ detection in larval developmental stages revealed that there were activated caspase from L1 to L3, indicating caspase is involved in the whole developmental process. The main apoptosis area was found in velum at L3 before metamorphosis, indicating the degradation of velum might be the process of apoptosis. The larvae before metamorphosis were treated by caspase specific inhibitor to analyze the role of caspase in this process. The results indicated that caspase have function metamorphosis.

根据caspase在不同物种中的保守区域设计简并引物,在文蛤幼虫中扩增到cDNA序列片段;检测有活性的caspase在文蛤幼虫各发育时期的分布部位,发现其在L1至L3期幼虫中都有分布,说明整个幼虫形态变化过程中都有caspase的参与;细胞凋亡检测结果显示,幼虫主要发生细胞凋亡的部位在L3期幼虫的面盘,即变态过程中要退化的器官,说明细胞凋亡可能是文蛤幼虫变态过程中面盘退化的主要机制;用caspase特异性抑制剂处理变态前幼虫,发现幼虫变态率下降,初步验证了caspase在文蛤幼虫变态过程中的作用。

Results Micrographic observation revealed that the Jiean-peptide made the hyphal expanded and globular.

结果 显微形态观察表明,经捷安肽素处理后,供试真菌呈现球形膨胀,而且随着捷安肽素作用强度增强,球形膨胀的程度和发生频率也增加,提示捷安肽素的作用位点在真菌细胞壁。

What we can conclude from the observation and seed-checking on morphic fectures of the treating group and comparison groups current generation and third generation is:apparent changes incurred to the leaf shape and the thickness of muskmelon when being treated by certain level of energy and dosage which can be inherited.

从各处理组与对照组当代和第三代苗期形态特征观察、考种中发现:在一定能量和剂量组合下,处理组香瓜叶型和果肉厚度发生了明显的变化,这种形态学变化是可遗传的。

Moreover, the actual allele frequency of most varieties deviates far from Hardy-Weinberg equilibrium. All PPB、na 、I、h、Gi and Fst have proved to be the references to elucidate that ISSR is a most powerful tool to analyze genetic diversity, compared with the RAPD marker and the allozyme marker is less strong ordinally. We could divided the 70 samples into A, B, C, D and E five groups using three methods according to genetic distance clustering. There is a bit displacement for few varieties in different clustering maps, but the most are similar to morphological analysis despite that there is still a great difference among cultivars in the same one group. The above results imply that the three methods have the different sensitivity and resolution in genetic distance analysis of close varieties. The Mantel test indicates that the results from the three kinds of markers have the significant correlation, which demonstrates that the number of the used three kinds of markers is enough to exactly detect the diversity of all 70 samples to ideal extent. And these methods can be used to evaluate the diversity of the whole group using the miscellaneous samples instead of the individual sample, of the Gerbera jamesonii are mainly from tissue culture plants. In conclusion, the above study results provide a reference for the application of three kinds of molecular markers to molecular marker-assisted breeding of flower. 2. The genetic diversity among the eight introduced cut-flower varieties of Ranunculus asiatica was analyzed by the ISSR markers. Based on the genetic clustering tree, all the colorful flower varieties are clustered into one group, and the white flower varieties into another group. Moreover, among the former group the yellow flower varieties are clustered into one sub-group, and the reddish flower varieties, such as rose color, pink, nacarat, are clusetered into another sub-group.

由三种分子标记的分析结果可以看出,等位基因平均值、观察杂合度、Fis值、Fit值皆较高,表明非洲菊等位基因较丰富,杂合基因偏多,且绝大部份品种的实际等位基因频率在品种内偏离了Hardy-Weinberg equilibrium;PP8、na、Ⅰ、h、Gi及Fst皆表明,ISSR检测遗传多样性的能力最强,其次是RAPD,等位酶最低;根据遗传距离进行聚类,三种方法都把70个品种分成A、B、C、D、E五个大组,每一组中除少数品种发生位移外,大部份品种分类结果相似,且与形态分析结果有相似性,但在每一组中,品种间的聚类差别较大,表明这三种方法在近距离品种间检测遗传变异时灵敏度及分辨力不同;Mantel检测表明,三种标记的分析结果有显著相关性,表明所用的三种分子方法的标记数量已经可以相对无偏地检测到70个品种间遗传变异;非洲菊为组培苗,三种标记的检测结果皆表明,混合样品可以作为个体样品的代表,对整个居群的遗传多样性进行评价;这些研究结果可为三种分子标记方法在花卉分子辅助育种中的进一步应用提供借鉴。

The comprehensive effect of partial acidlity of working medium, flowing status of fluid, nocuous material in atmosphere, together with the overlong usage, largely increased the corrosion rate and caused the leakage.

结果表明,泄漏的主要原因是焊接缺陷导致焊缝区发生缝隙腐蚀,而工作介质的局部酸性、流体的流动形态、大气中有害物质、温差应力等综合作用,加上使用期过长等加剧了腐蚀,导致该塔泄漏。

A development-course observation of the morphological changes in the nucellar cells undergoing PCD to form pollen chamber was performed.

在珠心细胞发生PCD的过程中,其形态变化显著,有序地降解细胞组分,其间液泡起着关键作用。

Results showed sTGC-CM, which is similar with the effect of hTGC-CM, was able to induce human DPSCs to make odontoblastic changes as indicated by remarkable morphological changes, increased proliferation ability, higher multipotential and colony forming capability, and expression of some odontogenic markers in gene and protein levels,such as DSP, DSPP, DMP1, BSP, OCN, OPN.

结果表明:人牙髓干细胞经过一定时间的猪牙胚条件培养基诱导后,能在细胞形态上和细胞周期上发生改变,能具有更高的克隆形成能力和多向分化能力,能维持更好的干细胞特性,具有更强的增殖能力,表达更高水平的碱性磷酸酶,免疫组化和RT-PCR提示诱导后的牙髓干细胞能在蛋白水平和基因水平表达出一些牙向分化的标志,如DSP、DSPP、DMP1、BSP、OCN、OPN等。

As a sexual reproductive organ, ovules have received much attention by botanists. Since the nineteenth century, the morphology, structure, ontogenesis, origin and molecular biology of angiosperm ovules have been studied in detail.

胚珠作为被子植物的有性生殖器官一直为植物学家所关注。19世纪以来,人们从形态结构、发生发育、起源及分子生物学等方面对胚珠进行了大量的研究。

Confocal laser scanning microscopy used to localize the hsp70 protein in oocytes and embryos revealed that the distribution of hsp70 in the ooplasm of immature and mature oocytes is unaffected by exposure to decreased or elevated temperatures and that this protein was closely associated with the meiotic spindle indicating its possible role in stabilizing this structure, hi eight-cell embryos derived under control conditions, hsp70 was evenly distributed in the cytoplasm but appeared as aggregates in some embryos exposed to elevated temperature.

此蛋白均匀地分布在两种细胞的胞浆中。此形态特点与减数分裂期纺缍体有关,由此表明hsp70在中期Ⅱ期,具有稳定这种结构的作用。在对照组8细胞胚中,hsp70均匀地分布在胞浆中,而在处理组一些胚胎内,hsp70则发生凝集。

Zn and Mn existed mainly as the oxidable form, and could be the free form under a high reduction condition, which had the potential harm for human and crop.

以这一形态存在的重金属,特别对环境变化很敏感,也就是说当土壤的pH值和氧化还原条件发生改变时,也可以被释放出来,具有一定的生态风险。

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Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

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