强地

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

Six strains of Phytophthora parasitica var. nicotianae were isolated from disease tobaccos in Yongzhou and Ningxiang from heavy disease plots;. Pathogenicity was tested to six strains, the results shows the strain of phy3 have strong pathogenicity. We also studied the effect of several solutions for producing zoosporangium in Artificial culture condition, the result shows the hyphea culture with 0.1% KNO3 produced the most quantity of zoosporangiums.

2001～2003年，从湖南永州和宁乡等地发病重的田块采集病株共分离到烟草黑胫病菌(Phytophthora parasitica var.nicotianae)菌株6株，经离体叶片致病力筛选，烟草黑胫病菌株phy3为强致病力菌株，病情指数达到89％；并在实验室条件下，研究了几种溶液诱导孢子囊的产生效果，结果证明经0.1％KNO，处理产生孢子囊最多。

Daughter of God and Man, accomplisht Eve, [ 660 ] Those have thir course to finish, round the Earth, By morrow Eevning, and from Land to Land In order, though to Nations yet unborn, Ministring light prepar'd, they set and rise; Least total darkness should by Night regaine [ 665 ] Her old possession, and extinguish life In Nature and all things, which these soft fires Not only enlighten, but with kindly heate Of various influence foment and warme, Temper or nourish, or in part shed down [ 670 ] Thir stellar vertue on all kinds that grow On Earth, made hereby apter to receive Perfection from the Suns more potent Ray.

这些星、月有它们固定的轨道，到明天的黄昏，绕地循环一周，依次巡行各地，所照耀的虽然还是未生出的民族，却预先为他们预备着光明，沉而复升，免得夜国占领全部黑暗，恢复她的旧地盘，使自然和万物的生命完全消亡；不但用这些柔和的火光照耀，而且用各种天然的暖气熏蒸、养育，或者在地上一切生物上面，洒落部分星星的效能，使它们更容易完全接受太阳更强的光线。

Identification and forecasting for igneous rock is one hard problem of exploration activity, because it has extreme aeolotropism. By firstly synthetically utilizing seismic analysis, well logging restricted inversion, core well core and electric log analysis method and so on, the igneous rock reservoir of Gaoqing oilfield is effectively identified and divided into one to four four phases according to igneous rock erupting phase.

由于火成岩具有极强的非均质性，对其识别与预测是油气勘探的难题之一，首次综合使用地震分析、测井约束反演、取心井岩心与电测曲线分析等技术和方法，有效地识别了高青油田火成岩储集体，并根据火成岩喷发期次，将高青油田孔店组火成岩储集体分为1、2、3、4期。

Alleged flexible language: It is to show with the target the market passes specific concept to appeal to to direct beg, the means of basis of fact of be particular about, engineer personnel by major or the literal advertisement that the article table of ad firm will come to organize responsible compose to have agitative to be not rigid medium, make the netizen walks into the magnetic field of my set not self-consciously, give its impact of powerful specific aim psychology, realize IP effect mode quickly.

所谓软文：是指以目标市场为导向通过特定的概念诉求、讲求事实依据的方式，由专业策划人员或者是广告公司的文案来组织负责撰写具有煽动性的非硬性介质的文字广告，使网友不自觉地走进我设定的磁场，给予其强而有力的针对性心理冲击，迅速实现IP效应模式。

This paper, aiming at the weak points of the low early strength and the slow setting and hardening of portland fly-ash cement , according to the forming mechanism of cement clinker minerals, cement clinker minerals are choosen, fly ash is used as a substitute of clay as raw materials, early and high strength type cement clinker has heen developed, the amount of alit in the clinker is very high.

本文针对粉煤灰水泥早强低，凝结硬化慢等缺点，根据水泥熟料矿物的形成机理，用粉煤灰代替粘土作原料，对熟料矿物进行优化组合，成功地研制出了一种高硅酸三钙的早期强度高的水泥熟料，这种熟料可掺入30％一40％粉煤灰，生产出425R型粉煤灰水泥。

Wild Alstonia scholaris grows in groups but comparatively loose, which can lead to the lack of wild Alstonia scholaris resources.

灯台树主要分布在乔木层（盖度≤54%）和灌木层（盖度≤37%）较为松散的森林和轮歇地、农用耕地等地段，具萌生力较强的特点。

DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

Suitable for planting grass in Qinghai are: Rhodiola rosea, Arenaria, Androsace, Leontopodium grass, grass seed, such as Sophie, the grass is to adapt to the harsh natural environment of the Qinghai-Tibet Plateau, they have strong vitality.

适合青海种植的草有：红景天、雪灵芝、点地梅、火绒草、柔籽草等，这些草都是为了适应青藏高原的严酷的自然环境，生命力很强。

Air and sea presence in Oceania would be a compromise approach between resisting a Greater China at all cost and assenting to a future in which the Chinese Navy policed the first island chain.

相比于不惜代价地阻止中国变强，或者同意中国海军将来可以在第一岛链巡逻这，加强美国在大洋洲的空军和海军势力是一个折衷的方式。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！