强取

GFP gene eukaryotic express plasmids were transfected into ES cells. The cells were observed under the inverted fluorescent microscope. The clones which expressed the powerful green fluorescence were chosen to be named ES-GFP cells and cultured continuously. The examination of ES cell totipotent including Alkine phosphatase staining, embryonic body formation in vitro and teratomas formation in nude mice was implemented.

1。采用脂质体方法将GFP质粒转染ES-D3细胞株，经筛选后倒置荧光显微镜观察细胞形态及荧光状态，挑取荧光最强的克隆，命名为ES-GFP细胞，进一步扩增培养，观察细胞生长及荧光表达情况，行细胞分化全能性鉴定包括碱性磷酸酶（alkine Phosphtase，AKP）染色、体外胚胎体（embryonic body，EB）形成实验及裸鼠体内成瘤实验。2。

Considering the hydrophobicity, a series of extraction equilibrium experiments for aniline and chloroanilines were carried out.

对疏水性较强的苯胺和氯代苯胺进行了物理萃取的研究。

The paper discusses the process of his architectural approaches harmonized between rural and urban enviroment, the strong character of anthropomorphism and metaphor in fis programs, the design sources from various aspects, as well as his significant contribution to the Organic Architecture Movement in Hungary.

本文介绍了他探求乡村与城市相协调的建筑创作历程，阐述了其建筑作品中的拟人化、隐喻性较强的个性特征和取自多方面的创作源泉以及他对匈牙利有机建筑运动的重要贡献。封面的毛科镇小剧院为I。马科韦茨1988年的作品

And close behind Johnson is Kurt Busch. He won the Sprint Cup in two thousand four.

科特 -布什在吉强森之后，他是 2004 年取得了 SPRINT 杯的冠军。

Recently, the test and estimation of segregation in asphalt pavements mainly rely on traditional methods as visual identification, sand patch testing, ground-penetrating radar and coring. Due to some disadvantages of these test methods metioned, such as slow test speed, subjectivity, deviation and some destructivity.

目前，对沥青路面离析的判定和检测方法主要依赖于观察法、铺沙法、路面雷达法和取芯法，这些方法存在主观性强、测速慢和误差大，且具有一定的破坏性。

In the particular ease where a and γ are equal to 1, the developable CE-Bézier surface is a developable Bézier surface. Finally, some applications in developable surfaces design are discussed, The modeling examples illustrate that the developable CE-Bézier surfaces provide two valuable ways for the design of developable surfaces.

由新方法生成的可展曲面不仅具有灵活的局部形状可调性和更强的描述能力，而且保留了CE-Bézier曲面的特性，特别是当α、γ都取值为1时，所生成的可展曲面即为CE-Bézier可展曲面。2种可展曲面设计方法的应用实例表明，该设计方法不仅简单、有效，而且易于控制曲面形状，从而为可展曲面的设计提供了一种新途径。

The eolian sand using in the experiment is taken from Xinjiang、Neimeng and Shanxi province.

试验所用材料全部取自新疆、内蒙、陕西三地沙漠中具有代表性的风积砂，具有较强的代表性。

Extracted from tea saponin, menthol and peppermint daidzin, the wild camellia essence made by brand new craft can deep cleanse scalp, improve head horny layer, inhibit epiphyte, remove heat, relieve itch, and remove scurf. Rich plant nutrition has good repairing function for dry, sallow and forktail hair caused by insufficient nutrition and perm. Its natural anti-oxidation ability can lastingly strengthen hair.

全新工艺萃取的茶皂苷、茶皂素、薄荷醇、薄荷异黄酮苷能深层清洁头皮、改善头部角质层、抑制真菌，可祛风散热、止痒、去头屑，其丰富的氨基酸及营养成分对因缺乏营养及烫染受损的干燥、枯黄、开叉发质有良好的修复作用，其天然抗氧化能力可持续强韧。

Take gravitational acceleration to be G, try the size that begs strong E of field of strong electric field of this divide evenly.

取重力加速度为 g，试求此匀强电场场强 E 的大小。

This experiment adopted organic solvent extraction step by step,following deproteinization,dialyzation,alcohol-precipitation and layer-separation of dextran,to reveal the unknown effective ingredients in pumpkin,which have influence to blood sugar in normal mice or model mice of diabetes mellitus.

为探寻南瓜中对正常及糖尿病模型小鼠血糖有影响的未知有效成分，本实验依次采用有机溶剂分步萃取、除蛋白、透析、乙醇沉淀及葡聚糖柱层析等分离手段，以小白鼠血糖值作为筛选活性成分的指标，在分离的每一阶段对分离所得各个组分进行活性定量评估，并追踪活性最强的部分，最后分离纯化出降血糖活性最强的组分G———南瓜多糖。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。