弱基

The effects of excess air coefficient and cylinder pressure on these spectra are also studied. The experimental resultS show that the three different combustion phases in a gasoline engine have different combustion characteristic spectra: a large number of atoms, molecules, ions and radicals which are in their excited states are existing during the ignition process, with the development of the combustion, the spectra intensities of CH, C2 radicals increase rapidly.

实验结果表明，汽油机三个不同的燃烧阶段具有不同的燃烧光谱特征：着火过程中，存在着大量的处于激发态的分子、原子、离子、自由基等活化中心的束缚态光谱，随着燃烧发展，CH、C2自由基的光谱强度明显加强；当减小过量空气系数时，光谱强度变弱并且着火延迟期增长；自由基特征光谱的光强变化曲线可以反映它们在燃烧过程中的浓度变化。

P28 紫色 Formation of a peptide bond 肽键形成–4侧链具有的反应–形成二硫键-其它反应 Tyr,His,Arg Review Isoelectric Point Titration滴定 of an amino acid 滴定 Gly幻灯片 18 His Lys Glu Reaction of amino acids Section 4 Protein isolation and purification 蛋白质分离及提纯 Working With Proteins Experimental techniques for protein analysis and characterization Purification steps A cell contains many types of proteins In the lab we want to isolate a single protein for experiments Purification steps We first grow cells or isolate tissues that contain the protein of interest We break open the cells to produce a crude extract Use centrifugation离心 to separate soluble from insoluble material We fractionate 分离 the protein mixture based on properties of such as size, charge affinity or solubility.

丹磺酰氯与氨基酸反应生成荧光性质强和稳定的磺胺衍生物，用于多肽链NH 用于多肽链 3末端氨基酸的标记烃基化反应(1) 2，4-二硝基氟苯2，4-dinitrofluorobenzene，二硝基氟苯(二硝基氟苯 DNFB也叫做试剂。DNFB在弱碱性溶液也叫做Sanger试剂试剂中与氨基酸发生取代反应，生成黄色化合物二硝基二硝基苯基氨基酸(dinitro phenyl amino acid， DNP氨基酸氨基酸)苯基氨基酸氨基酸(2)苯异硫氰酸酯(phenylisothiocyanate， PITC)在弱碱性条件下，与氨基酸反应在弱碱性条件下，生成苯乙内酰硫脲 PTH衍生物，(phenylthiohydantoin， PTH)衍生物，即PTH-氨基酸，此反应又称之Edman反应，该反应是蛋白质或多肽氨基酸序列测定常用的反应。

So it is assumed that the E-box might be associated with the enhancement of transcription activity.Here, the LTRs of WH17 ,DLA-25,DLA-118 and LTR with single mutation in the E-box motif were separately cloned into pCAT-Basic vector resulting in a series of recombinant plasmids containing CAT reporter under the control of different versions of LTRs.

通过对EIAV分离株WH17的LTR与L株、DA和DLA株的LTR序列进行比较，发现其在在U3-R结合处多一个E-box基序，推测该基序的变化可能会起到促进转录作用，为此将EIAV强毒株(DLA-25)、驴白细胞弱毒株(DLA-118)、EIAV分离株WH17以及U3-R结合处的E-box基序点突变的LTR分别克隆到pCAT3-basic质粒中的报告基因氯霉素乙酰转移酶的上游，获得一系列受不同LTR控制的CAT表达质粒。

We also obtain a new judgement theorem for hereditarily metaLindelof space and paracompact space. In addition, we give a depiction for g-metrizable space from a new direction with σ—ωHCP closed weak base.

f空间和仿紧空间的判定方法，并给出了g-可度量空间关于σ-弱遗传闭包保持闭弱基的一个刻画，进一步丰富和发展了广义度量空间的基本理论。

In this paper the thymus DNA of calf and the pBs plasmid are the main objects of our studies in ultraweak luminescence. By means of PIAS(Photon-counting image acquisition), ultraweak luminescence detector and Lambda Bio 40 of UV/VIS spectrophotometer, we studied the UL of DNA induced by the three oxygen free radicals: singlet oxygen Oa, hydroxy radical and superoxide anion(O2 ). We found that the OH can greatly increase the UL intensity of DNA.

本论文以小牛胸腺DNA和质粒pBs为实验材料，利用光子图像探测系统(Photon-counting image acquistion，PIAS)、微弱发光测量仪和UV／VIS分光光度计和生物统计学的成组比较法原理对单线态氧(~1O_2)、羟自由基、超氧阴离子(O_2~-)三种氧自由基诱导DNA超弱发光的变化过程进行了研究，结果发现这三种氧自由基中·OH能诱导DNA的超弱发光发生显著的增强变化，而其他两种氧自由基对DNA的超弱发光并无显著诱导作用。

In the three systems, system realizes the crosslinking of PVC bases on the peroxide crosslinking mechanism, the tensile strength of crosslinked PVC has been improved somewhat, the IR spectrum of gel demonstrates that there are characteristic absorption peaks of n-alcohol that belongs to ethanol amine and benzenoid group. From the analysis of gel structure , deduces that the mechanism of crosslinking reaction: under the initiations of peroxide and high temperature, PVC yields macromolecules free radicals, at the same time , styrene"s polymerization and grafting onto PVC takes place, at last the system forms crosslinking structure through coupling between radicals; confirms that triethanol amine can absorb HC1 produced by degradation of PVC, so improves the crosslinking efficiency of peroxide, decreases the HCl"s promotion for PVC"s degradation; deduces that triethanol amine can activate the PVC macromolecules and prolong the free radicals" life-time of PVC macromolecules; the effect to the PVC macromolecule makes styrenes graft reaction easy, and thus improves the crosslinking efficiency.

三种体系中三乙醇胺/过氧化二异丙苯/苯乙烯体系基于过氧化物交联机理实现了聚氯乙烯的交联，聚氯乙烯交联后拉伸强度有一定提高，凝胶红外光谱分析证实了凝胶结构中含有乙醇胺的伯醇基团特征吸收和苯环的特征吸收，由凝胶结构分析，推断出交联反应的机理：聚氯乙烯在过氧化物和热作用下产生大分子自由基，苯乙烯在过氧化物和大分子自由基的作用下发生聚合和接枝反应，最终通过自由基之间的偶合产生交联；证实三乙醇胺可以吸收聚氯乙烯分解产生的HCl，提高了过氧化物的交联效率，消弱了HCl对聚氯乙烯降解的促进作用；推断三乙醇胺可以活化聚氯乙烯大分子并有可能使聚氯乙烯大分子自由基存在的时间得以延长，对聚氯乙烯大分子的作用使苯乙烯的接枝反应易于发生，从而提高了交联效率。

It indicate that 200μmol/m2s and 80μmol/m2s low light cause reactive oxygen species quick producing and SOD and CAT acticity enhance, but the latter's increasing cann't offset reactive oxygen species'quick producing, which lead to membrance peroxidize injury on peach tree. 30μmol/m2s low light's injury on peach tree mainly because reactive oxygen species'accumulation.

表明200μmol/m2s和80μmol/m2s的弱光下桃树抗氧化酶活性的诱导提高不足以抵消弱光下自由基的快速生成，从而导致对桃树细胞膜脂的伤害；30μmol/m2s的弱光下桃树活性氧的快速生成加剧膜脂过氧化程度，是造成弱光对桃树伤害的重要原因之一。

It can be proposed that when the substituent is an electron donor, the twisted configuration between pyrazoline ring and the moiety attached on N1 is the fluorescence-emitted configuration of 2-pyrazoline, and the configuration where two moieties are placed in a co-plane is the best non-radiative decay configuration of these compounds.

这表明，对于C3位取代基为推电子基或弱的拉电子基团的化合物，N1所联取代基与吡唑啉环相扭曲的构象是化合物的发光构象，两者处于同一平面的构象是化合物的最佳非辐射衰变构象，与C3所联取代基的旋转扭曲无关。

The results of calculation will be fundmentally consistent with the experimental values.In addition, we calculated dibenzene chromium and its derivatives, ferroceneand its derivatives. The former had higher symmetry. We studied the substituenteffects by calculating their NMR spectra. The results show that: when we add asubstitute group on one ring of dibenzene chromium, the substitute group will affecttwo benzene rings. The effect is similar to the effect of branched alkanes, namely:the effect on neighbouring groups has transmissibility. Furthermore, the nearer theneighbouring group is, the stronger the effect is. This effect is observed not only onthe substituted ring, but also on the unsubstituted ring.

此外，我们重点计算了二苯铬及其衍生物和二茂铁及其衍生物的核磁共振谱，其中二苯铬及其衍生物选择的是对称性较好的体系，通过计算其核磁共振四川师范大学硕士学位论文谱，探讨其取代基效应，结果表明：在二苯铬的一个环上引入取代基后，取代基对两个苯环的影响，类似于支链院烃引入取代基后的影响：即对邻近基团的影响有传递性，并且对离得近的作用强，对离得远的作用弱，这种作用不仅体现在连有取代基的环上，也体现在没有带取代基的环上。

First, the significant of co-factor InsP6 and center water in the mechanism of auxins are explored by docking; second, the experiments that compared with the docking rusults of two situations involving fully rigid and selective flexible of active residue of the receptor TIR1 illuminate that selective flexibility docking by AutoDock4 reports more rational results, so that, AutoDock4 dockings are implemented with TIR1-Auxins; third, AutoDock4 docking by TIR1-Auxins-Aux/IAA illuminates that auxin as a molecular glue enhances the interaction between TIR1 receptor and Aux/IAA substrate by the weak interactions, such as hydrogen bond and hydrophobic interaction, furthermore, the weak interactions between receptor protein and ligands greatly influence on auxin activity of auxin ligands.

首先， 通过分子对接计算研究辅酶InsP6以及中心水分子在生长素反应中的重要作用；其次，比较受体大分子完全刚性以及活性残基部分柔性的两种情况下的分子对接结果，说明AutoDock4实现了受体分子活性残基的部分柔性而使对接结果更加合理，进而使用AutoDock4方法对TIR1-Auxins体系进行对接计算；最后，对TIR1-Auxins-Aux/IAA体系进行分子对接计算，结果表明，生长素配体分子作为&分子胶水&直接与受体大分子TIR1以及底物多肽Aux/IAA形成强的弱相互作用，如氢键作用、疏水相互作用，促进了受体TIR1与Aux/IAA底物之间的结合，进而说明氢键作用和疏水相互作用等弱相互作用对于生长素分子的活性具有很大的影响。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失