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A kind of virus particles were observed in cytoplasm of sampled ill sea cucumber tissues which were sampled from sick sea cucumbers with ulcerous skin and swollen mouth from their farming ponds at the beginning of 2005. The particles were covered with a bilayer envelops, shaped in globular with its size 100-200 nm in diameter. The same virus was isolated from cultured sea cucumbers with disease from different time and sites, and then infected the healthy sea cucumber. sea cucumber appeared the phenomenon such as ulcerous skin and swollen mouth and the same virus was isolated from infected sea cucumbers.

本章从患病刺参中分离出了2株对刺参具有感染能力的细菌，经鉴定1株为弧菌科弧菌属Vibrio sp和1株为弧菌科施万氏菌属Shewanella sp；在患病刺参的触手、肠和呼吸树等组织的细胞质中，检出了球形、具双层囊膜、直径大约为100-200nm的病毒粒子利用分离的病毒进行人工感染实验，刺参可以产生与自然发病刺参相同的症状，显示了分离的病毒对刺参具有一定的感染性。

As a result of using 4 species pathogen probes,the positive rate of Vibrio alginolyticus and V. vulnificus is 3.4%.

在常见的4种致病性弧菌的检测结果上，创伤弧菌和溶藻弧菌阳性率均为3.4%。

Through the comparative analysis that: the arc-tooth flow of hydraulic performance is better than the arc, Adopting optimize the design parameters of the structure, reducing the flow rate of 0 regions, and strengthen vortices regions, improved irrigation arc tooth for hydraulic performance and anticlogging ability, irrigation design recommended by arc-tooth flow structure.

通过对比分析认为：弧齿形流道水力性能优于弧形流道，可以通过结构参数优化设计，减小0流速区域，强化旋涡区，提高弧齿形灌水器的水力性能与扰堵塞能力，灌水器设计中推荐采用弧齿形流道结构形式。

Vibrio s are defined as Gram-negative, asporogenous rods that are straight or have a single, rigid curve.

弧菌是革兰氏阴性、不产孢子的棒状或弧状的细菌，可以运动，许多弧菌在液体培养基条件下生长时具有一个极性鞭毛。

It was demonstrated that BDF-H16 was an gram-negative bacterium and had a bacilliform or arc bacilliform shape with a flagellum at one end.

结果表明：蛭弧菌BDF-H16为革兰氏阴性菌，杆形或弧杆形，端生一根鞭毛，菌体大小多为0.2μm～0.5μm×0.8μm～1.2μm；蛭弧菌BDF-H16对实验所选用的革兰氏阴性菌和部分革兰氏阳性菌均有裂解作用；以大肠杆菌为宿主菌，其最佳生长条件为宿主菌浓度6.75×10~9cfu/mL、pH7.0～7.5、温度28℃；在NaCl含量0.85%～5.00%的培养基中能够生长；恩诺沙星、诺氟沙星对其有抑制作用。

It was demonstrated that BDF-H16 was an gram-negative bacterium and had a bacilliform or arc bacilliform shape with a flagellum at one end.Its size was mostly 0.2μm～0.5μm×0.8μm～1.2μm.It had a wide prey area and could lyse all tested gram-negative bacteria and some gram-positive bacteria. The best lysis conditions to Escherichia coli were 6.75×109cfu/mL of prey bacteria concentration、pH7.0~7.5、28℃. It could grow in the solid culture added 0.85%~5.00% NaCl and was inhibited by enrofloxacin and norfloxacin.

结果表明：蛭弧菌BDF-H16为革兰氏阴性菌，杆形或弧杆形，端生一根鞭毛，菌体大小多为0.2μm～0.5μm×0.8μm～1.2μm；蛭弧菌BDF-H16对实验所选用的革兰氏阴性菌和部分革兰氏阳性菌均有裂解作用；以大肠杆菌为宿主菌，其最佳生长条件为宿主菌浓度6.75×109cfu/mL、pH7.0～7.5、温度28℃；在NaCl含量0.85%～5.00%的培养基中能够生长；恩诺沙星、诺氟沙星对其有抑制作用。

The maximum perturbation accelerations and their percentage contribution in the sum of all accelerations due to the lunisolar perturbations,Earth tides,ocean tides,atmospheric tides,pole tides,relativity effects,atmospheric drag and solar radiation pressure are computed on the basis of simulated orbit.

结果表明：①在1.0ⅹ10-9m/s2的非保守力测量精度内，除大气潮可忽略外，其余摄动必须考虑；②考虑GOCE预期定轨精度(3 cm)，在弧长小于2.0 h的短弧定轨中，固体极潮和大气潮的影响可忽略，在长弧定轨中，忽略任何一种保守力的轨道预报时间均不足0.5 d；③各种潮汐对重力梯度观测值的影响量级均小于GOCE预期的测量精度(3 mE)。

Vibrio cholerae adherence to and colonization in the small intestine are essential step in the pathogenesis of cholera. At least one type of pili, known as TcpA, is required for the colonization process. This paper reports the electron microscopic evidence showing that V. cholerae 01 and 0139 serogroups can express at least 4 types of pili when incubated at 28℃ on AKI agar or CFA (pH6.8). Among the four types, three can be expressed by classical and El Tor strains of Vibrio cholerae 01: TcpA pili are peritrichous,being 7-8nm in diameter, 5-10μm in length, and form bundles of parallel undulating filaments of 0.5-0.8 μm wide and 10-15μm long; Type B are 3-4nm in diameter, up to 2μm long, and of wavy morphology; Type C are 7-8nm wide and 0.5-0.8μm long.

本文通过电子显微镜对01群霍乱弧菌古典生物型和El Tor生物型以及0139群霍乱弧菌研究发现，在培养基AKI和CFA(pH6.8)28℃培养条件下，01群EVC和CVC均可产生TcpA，B型和C型菌毛；0139群霍乱弧菌可产生TcpA和B型菌毛，与01群一致。

At last, arc reignition characteristic is defined for multiple-narrow slot chamber, and the mechanism of arc reignition process is analyzed. A mathematical model which forecasts the arc reignition process is improved for the first time by combining the striking theory of cathode layer with"black box"model for forecasting arc thermal reignition.

最后，通过试验确定了多纵缝陶土灭弧室中电弧重燃特性，对其机理进行了研究，并首次将阴极层击穿理论和预测电弧热重燃的黑盒模型相结合，建立了模拟陶土灭弧室中弧隙由电击穿到热重燃过程的电弧数学模型。

To obtain the tervalent fusion toxin gene, three toxin gene fragments from three species of Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio mimicus were connected with the flexible linker using overlap extension PCR. The three toxin gene fragments respectively encode the mature proteins of the thermostable direct hemolysin of V. parahaemolyticus, the cytotoxin of V. vulnificus and the heat-labile hemolysin of V.

采用柔性Linker和重叠延伸PCR方法将三个毒素基因，即副溶血性弧菌的耐热型直接溶血素基因tdh、创伤弧菌的溶细胞毒素基因vvhA和拟态弧菌的热不稳定型溶血素基因vmhA的成熟蛋白基因片段进行串联，得到三联融合毒素基因tdh-vvhA-vmhA。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。