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In 66 anaesthetized rats, the effects of local injection of agmatine on femoral, renal, and mesenteric vascular beds were investigated respectively by constant flow perfusion method.

在66只麻醉大鼠,分别采用后肢、肾脏和肠系膜动脉在体恒流灌注法,观察了向灌流环路中直接注射胍丁胺(agmatine, AGM)的血管效应,以所引起的灌流压增减反映血管的收缩和舒张。

Methods The constriction induced by norepinephine (NE, 10-8-10-4 mol/ L) and diastole induced by acetylcholine (Ach, 10-8 -10-4 mol/ L) in thoracic aortic ring with angiosteosis induced by vitamin D3 plus nicotine in rats were recorded by BL-Newcentrary multichannel physiologic recorder.

维生素D3(VitD3)和尼古丁诱导大鼠血管钙化模型,通过BL-Newcentrary型多导生理记录仪,观察钙化大鼠离体胸主动脉环对去甲肾上腺素(norepinephine,NE)(10-8~10-4mol/L)和乙酰胆碱(acetylcholine,ACh)(10-8~10-4mol/L)引起的收缩和舒张反应。

objective to observe the protective effect of qiliqiangxin capsule in rabbit heart ventricle reconstitution of chronic heart failure.methods using the method of giving rabbit abdomen aorta half ligations to build the chronic heart failure model to observe qiliqiangxin capsule the effect to blood flow mechanics index,room wall thickness,heart exponent and heart ventricle reconstitution biochemistry index.results qiliqiangxin capsule could reduce effectively bp,lvsp,lvedp,t wave,improve room wall thickness and heart exponent,reduce angii and alt,all the paramenters ahd notable difference,composed to those of model group (p<0.05,p<0.01).conclusion qiliqiangxin capsule can improve the blood flow mechanics change of chronic heart failure rabbit,which has notable inhibitory action on heart ventricle reconstitution which originate from chronic heart failure.inhibition of ang ii,alt of immune nerve endocrine system may be a mechanism of qiliqiangxin capsule.

观察血流动力学、标志心室重构的室壁厚度及心脏指数及肾素―血管紧张素―醛固酮系统相关生化指标的变化。结果芪苈强心胶囊可有效降低血压、左室压、左室末期舒张压、t波,改善室壁厚度和心脏指数,降低血清血管紧张素ii及醛固酮水平,与模型组比较均有显著性差异(p<0.05,p<0.01)。结论芪苈强心胶囊可改善慢性心力衰竭兔血流动力学的变化,对慢性心力衰竭引起的心室重构具有显著抑制作用,其机制可能与该药发挥抑制神经内分泌免疫系统rass中angii、ald的水平有关。

Methods The changes of contents of AGEs in plasma and the abdominal aorta and changes of VSMC of the abdominal aorta were investigated with methods of fluorospectrophotometry, immunohistochemistry, computer image analysis and transmission electron microscopy on streptozotocin-induced diabetic rats. Results From the fourth week, in diabetic rats, the contents of AGEs in plasma and the abdominal aorta increased, the relative area of α-SM-actin in the abdominal aorta decreased, the density of nucleus of VSMC increased, and mitochondria and rough endoplasmic reticules in VSMC increased.

后者在早期主要是血管平滑肌细胞(vascular smooth muscle cell, VSMC)和胶原纤维增多,弹性纤维减少,动脉增厚等,引起动脉的顺应性降低,舒张功能减弱[1~3];晚期主要表现为动脉粥样硬化和动脉硬化,其中以VSMC的增生和迁移较为突出,其表型也由收缩型转化为合成型[4,5]。

Then the responses of artery to phenylephrine acetylcholine, sodium nitroprusside and norepinephrine were studied.

结果:转基因血管内皮细胞的增殖较对照组活跃,动脉的内皮依赖性舒张反应较强,10 -6mol/L 的乙酰胆碱引起的舒张为33-08%±10-01%,对照组为- 17-57%±5-59%,P< 0-01 。

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