引物原位标记

Objective: To explore the reliability of the method PRINS for detection of spermic aneuploidy .

目的：探索应用引物原位标记技术分析精子染色体非整倍性的可靠性。

Objective To use a rapid method by PRINS for detection of spermic aneuploidy.

目的利用引物原位标记技术快速检精子染色体非整倍性。

It even can be seen that the disomy rates of both two donors were higher than the diploidy rates, which may imply that more disomy occurred than disomes in meiosis process.

结论引物原位标记技术看起来是检测人类外周血淋巴细胞中期染色体和精子染色体数目异常的一项经济简便和快速可靠的高通量检测方法。

Objective: To explore and evaluate a multicolor PRINS protocol for chromosome detection in human sperm .

目的：探索和评估应用于精子染色体数目异常检测的多色引物原位标记技术。

We have developed a technique of random-primed tritium labeled DNA extension on the pachytene bivalents of rice-field eels.

采用随机引物，在黄鳝二价体上建立了氚标记引物原位DNA合成技术，并详细探索了该技术的实验条件。

The dark bands alternating with light bands,relatively stable and R-band-like structure was showed on the pachytene bivalents of Rics-field eelby using the random-primed in situ digoxigenin-labelled DNA synthesis technique,and the results were analysed and discussed in detail.

在黄鳝二价染色体上，运用地高辛配基标记的随机引物原位DNA合成技术诱导出了明暗相间、基本稳定且类似于R带的带状结构。本文对该结果进行了较详细的分析和讨论。

We successfully identified JSRV mRNA expression and proviruses DNA in sheep lung tissues infected with JSRV and control group has no postive signals,Validating the use of exogenous virus-specific DNA probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences.

用地高辛随机引物法标记exJSRV特异的env片段，制备探针，原位杂交检测SPA肺组织中的RNA及前病毒DNA，结果表明SPA患羊肺组织内有JSRVenv基因mRNA的表达，同时也检测到了前病毒DNA，而相应的阴性对照却无阳性信号，证实外源性病毒特异的DNA探针在致瘤性前病毒的整合位点和整合的外源性前病毒的检测中具有可信度。

In this study, multiple primer polymerase chain reaction , consensus primer PCR and in situ hybridisation with biotin-labelled DNA probe (using domestically-produced agents) were applied and the sensitivity, specificity as well as practical value of each method were compared.

本实验采用了国内外领先的多重聚合酶链反应（polymerase chain reaction，PCR）和共用引物PCR法及国产生物素标记探针原位核酸杂交法，比较和估价了三种方法的灵敏度，特异性和在实际工作中的应用价值。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。