引人的

We choose abortus of three to six months old as research object. In the research, we collected 35 specimen that all came from induction of labor with water bag. We successfully culture OECs from human embryo olfactory bulb by using primary culture.

一、人胚胎嗅神经鞘细胞的体外培养本研究共收集标本35例，均为3—6个月的水囊引产流产儿，采用原一代培养的方法，自人胚胎的嗅球培养出OECS。

We hereby give you notice that pursuant to a deed of charge and assignment dated 28th July 09 entered into by us in favour of Standard Chartered Bank (the "Offshore Security Trustee") we have assigned to the Offshore Security Trustee as trustee for the Secured Parties referred to therein (the "Secured Parties") all of our light, title and interest in and to the Turnkey Agreement made between A Company and B Company (the "Agreement") and all present and future rights and benefits thereof and all moneys and proceeds paid or payable thereunder.

我们以此方式让步你注意那个依照a契约的收费和任务过时9年7月28日输入变为在附近我们进入恩惠的标准发给特许证堆积"向海安全当受托人"我们富人分配向向海安全当受托人某样的人当受托人为使牢固Parties引去参考向在其中"使牢固Parties"所有的我们点燃题目和权益在朝派和向监狱看守协议制做在中间A公司和B公司"协议"和所有的指向和未来rights和benefits其所有的大笔的钱和收益付在其下可以支付金色。

Every year, millions of smokers die because of illness which are caused by smoking tobacco.

每年有数以百万计的人因吸烟而引起病死亡。

In addition to solo soprano and bass, four-part chorus, and a full orchestra with paired woodwinds and four horns, Beethoven calls for piccolo and percussion -- instruments associated by the Viennese with Turkish Janissary bands. This instrumentation would reappear in Beethoven's Ninth Symphony, in the Turkish March variation of the finale.

在两千年后当她被合唱团唤醒回到雅典城后，该城却不再是她记忆中的辉煌样貌，而是一片废墟，而希腊人早已沦为奴隶，被异国的土耳其人和回教徒所统治，希腊人引为自豪的艺术和科学，早已荡然无存，但匈牙利却保存了下来。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

The feminism is not to represent the rights struggle and the sexual confrontation, or to obliterate the sexual difference indiscriminatingly; but to understand human beings more comprehensively through concrete and sexual individuals.

女性主义主要不是表现权利的斗争和两性性别对抗路线，它也不是以笼统的人去抹杀性别差异，而是通过具体的、有性别的人对人类进行更全面的理解和把握。黄引的摄影作品《性。

This time, we co-organize with PolyU Katso and the gathering was held in N113 room of PolyU from 7:30 to 9:15. We had around 23 people coming.

然后上主天主用那由人取来的肋骨，形成了一个女人，引她到人前，遂说：「这才真是我的亲骨肉，她应称为「女人，」因为是由男人取出的。

For the gate is narrow and the road is hard that leads to life , and there are few wh o find it .

路是大的，进去的人也多；引到永生，那门是窄的，路是小的，找着的人也少。《新约·马太福音》第7章

What irony if China, which takes pride in its own recent manned space flights, were to find its ambitions to put a man on the moon and eventually to build a space station set back someday by the still ricocheting rubble from its own irresponsible action.

中国虽然对前不久的载人飞行器引以为傲，一旦发现要把人送上月球并最终建立空间站的雄心壮志却因自己不负责任的举动造成的飞沙走石而告吹的话，那可是够讽刺的。

Such expectations rose earlier this month, when the kingdom's most senior cleric, the Grand Mufti, told Mr Ghamdi to keep his opinions to himself, saying that anyone who questioned the need for communal prayers was leading Muslims to hell.

本月初，王国的最高牧师大穆夫提告诉Ghamdi先生，不要宣扬他的观点，任何质疑公共祷告必要性的人都在将穆斯林人引向地狱。这无疑增加了Ghamdi先生被解雇的预期。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。