引

Another pair of primers including the start codern and the terminer coden were designed to amplify the PAL gene. Two restriction enzyme XbaI and XmaI were added to the two primers respectively, which enable to insert this fragment to the prokaryotic expression vetor pCAMBIA2301-WCS.

在PAL 基因的编码框以启始密码子ATG 和终止密码子TAG 为两端设计一对特异引物，分别在引物5' 端引人XbaI 和XmaI 酶切位点，用来扩增PAL 基因并与pCAMBIA2301-WCS 构建表达载体pCAMBIA2301-PAL。

To observe and analyze the time of chest tube insertion,the intensity of resistance to pulling and drainage effect between the thoracentesis technique of invented minute invasion closed-chest drainage and traditional closed-chest drainage.

利用家犬自体股动脉穿刺采血注入一侧胸腔、同时注入等量盐水及空气的方法建立家犬血气胸模型，并将其随机分成实验组（n=12）和对照组（n=12），观察分析自制微创胸腔闭式引流装置与传统胸腔闭式引流装置的置管时间、抗拉强度、引流效果等。

A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果：1、用PCR方法检测A组链球菌：以A组链球菌致热性外毒素基因speB为靶序列，设计的扩增引物对全部对照菌株的扩增结果为阴性，而全部A组链球菌参考株均能扩增出特异的345bp片段，其中包括三株猩红热链球菌，检测敏感性为6.5pg／μl DNA.2、用PCR方法检测白喉杆菌：以白喉外毒素基因toxB为靶序列，设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段，而全部对照株的扩增结果为阴性，检测敏感性为850fg／μl DNA.3、用PCR方法检测嗜肺军团菌：以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因，设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段，而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

In this thesis, we mainly expatiate on the design of the wefting mechanism and the shuttle box of the heavy wide loom, the prototype is JURGENS loom and we subdivide the wefting mechanism and the shuttle box in the modular way. There are shuttle box, weft carrier, wefting mechanism, braking mechanism, abb joining mechanism, unreel and tension control mechanism and so on.

因此，本文论述的重点便放在重型宽幅织机的引纬机构与梭箱部分，设计中以JURGENS织机为原型，采用模块化的方式细分引纬机构与梭箱，其中包括梭箱、引纬器、投梭机构、制梭机构、纬纱交接机构以及纬纱的退绕与张力控制机构等部分。

A model ejector with variable geometrical throat was designed, then, the processes of start and unstart were investigated, and a criterion was proposed for the condition that second throat is too small to start.

设计了几何结构可调的模型引射器，研究了引射器的启动和不启动过程，提出了判断第二喉道面积过小导致引射器无法启动的判据。

Air-entraining agents of different composition were used in ordinary concrete to determine their effects on air content and bleeding capacity of concrete, therefore suitability of the new air-entraining agent to concrete could be proved, and then strength and frost resistance of optimum air entraining agent were tested.

通过将不同组成的引气剂应用于普通混凝土中，测定其对混凝土含气量和泌水率的影响，从而证明本研究所研制的新型引气剂在混凝土中的适用性，再针对最佳引气剂进行强度和抗冻性的测试。

In this dissertation, the design principle of the approach of typical fuse-warhead matching was given. According to the principle, this dissertation constructs four kinds of matching ways of fuse with warhead on the basis of obtaining necessary intercrossing information.

文中还给出了典型引战配合逼近的设计准则，并在获取必需的交会信息的基础上，依据典型引战配合逼近的设计准则，构造了四种不同的引战配合方式。

Methods To divide randomly 150 women of 14～18 week pregnancy who volunteer to cease conception into three different roups:A,B and C,namely group by rivanol,group by mifepristone combining with misoprostol,and group by mifepristone plus rivanol,and observe the induction effect,time of commencement of uterine contraction,time from uterine contraction to fetal discharge,and quantity of bleeding on induced labor,etc.

目的 探讨对14～18周妊娠几种不同引产方法的效果。方法将150例妊娠14～18周自愿要求终止妊娠者随机分成A、B、C3组，即利凡诺组、米非司酮联合米索前列醇组、米非司酮加用利凡诺组，观察各组的引产效果、宫缩发动时间、宫缩到胎儿排出时间、引产出血量等情况。

Cerebral thalamus and basal nuclei hemorrhage patients with haematoma breaking into lateral cerebral ventricle and the third and fourth ventricle were treared by being put tube into two cornu frontale ventriculi lateralis and haematoma cavityt.

人流没有138例中血肿经过充分引流，奎根试验，夹管试验等措施，如闭管后病人病情加重，可暂时开放引流管并再次注入尿激酶后引流，确认可以拔管后有2例再次置外引流管血玉咒，1例因不能拔管行v-p术。

In the barley, the oats, the triticale and three awn grass, the success ratio of these primers respectively was 73.2%, 82.9%, 87.8% and 85.4%, and some primers even still appeared specificity strip bands in particular plant, These results indicate that the SSR marker coming from the wheat gene set is highly transferable among species in different genus of Pooideae.

所选取的41对引物在大麦、燕麦、小黑麦和三芒草4种供试植物中的扩增成功率分别为73.2%，82.9%，87.8%和85.4%，有些引物在特定植物中还会出现特异性条带，以上结果表明，从小麦基因组上开发的SSR引物，在早熟禾亚科不同种属植物间有较高的通用性。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。